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The map label for this gene is hpaIM [H]

Identifier: 159896851

GI number: 159896851

Start: 379482

End: 380330

Strand: Direct

Name: hpaIM [H]

Synonym: Haur_0318

Alternate gene names: 159896851

Gene position: 379482-380330 (Clockwise)

Preceding gene: 159896850

Following gene: 159896855

Centisome position: 5.98

GC content: 53.0

Gene sequence:

>849_bases
GTGAATTCGCAGCAATCAAATCAGCTTATCCAAGGCGAAATGCTCGATGTTATCGGCTCAATCGCGCCTCAATCGATCAA
TCTGTTGTATCTTGACCCGCCATTTGCTGCTGGTCGGGTGTTTGCCGATAGCGCTGGAGCTTTTGATGATCGTTGGCAAG
GTGGCTTAACTGAATATTTGGCTTGGCTTGAGCAACGTTTGGTGGCTGCTCGCCGAATTGTGGCTGAGCATGGCTCGTTA
TTTTTGCACCTTGATCGGCGGGCGGTGCATTATGCCAAAGTGCTGCTTGACCAAACGTGGGGTTTCGAGTGCTTTCGCAA
CGAAATTATTTGGCATTACACAGGCGGCGGGCGAGCACGACGCAGTTTTTCGCATAAACACGATACAATTTTGTGGTATA
GCAAACATCCGCAACACTGGACATTTAATCTCCAAGCAATGCGTCAACCGTATAAAGCCAGCAGTGGTTTTGCCAAGGCC
GGCATTCGTTCGGCGGCTGGCAAGCGTTATTTGCCGCATCCCGATGGCACGCCCGTCGATGATGTTTGGGATATTCCGAT
GCTCAACCCCATGGCTGCTGAACGTTTGGGCTACCCCACCCAAAAGCCCGAACGCTTGCTGGAGCGAATTATTCTCGCAG
CAAGCAATTCAGGCGATCTGGTAGCTGATCTCTGTTGTGGCTCAGGCACGACGGCGGCAGTGGCTCAGCGCCTAGGGCGA
TGCTGGATTGCAGCAGATCAATCGGCTGATGCCTTGGCTTTGGTGCGTGGTCGCTTGCGCCAACAAGCTGCCGAATGGCA
AGAGACGCTTATCCAACCTCAGCCAGCTTCGTTTGCTGCCAAGCGCTAA

Upstream 100 bases:

>100_bases
ACTAACATTGAGCAAGATCTCTATCAACTTGAAACGACCAGTCGCGAACGCTTGGCCAATTTGTTGATTCAATCGTTAGC
ATAGTTTAGAAGGGCTTGGC

Downstream 100 bases:

>100_bases
GCGCCGTCGCAGCCACTGCTGAATCCCAATGGTGATCAATCGCTTGATCGATTAAGCCTTGCAGATTGGGGCTAAGGCTG
CTTGGTTGTGCTCGCAAATG

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNSQQSNQLIQGEMLDVIGSIAPQSINLLYLDPPFAAGRVFADSAGAFDDRWQGGLTEYLAWLEQRLVAARRIVAEHGSL
FLHLDRRAVHYAKVLLDQTWGFECFRNEIIWHYTGGGRARRSFSHKHDTILWYSKHPQHWTFNLQAMRQPYKASSGFAKA
GIRSAAGKRYLPHPDGTPVDDVWDIPMLNPMAAERLGYPTQKPERLLERIILAASNSGDLVADLCCGSGTTAAVAQRLGR
CWIAADQSADALALVRGRLRQQAAEWQETLIQPQPASFAAKR

Sequences:

>Translated_282_residues
MNSQQSNQLIQGEMLDVIGSIAPQSINLLYLDPPFAAGRVFADSAGAFDDRWQGGLTEYLAWLEQRLVAARRIVAEHGSL
FLHLDRRAVHYAKVLLDQTWGFECFRNEIIWHYTGGGRARRSFSHKHDTILWYSKHPQHWTFNLQAMRQPYKASSGFAKA
GIRSAAGKRYLPHPDGTPVDDVWDIPMLNPMAAERLGYPTQKPERLLERIILAASNSGDLVADLCCGSGTTAAVAQRLGR
CWIAADQSADALALVRGRLRQQAAEWQETLIQPQPASFAAKR
>Mature_282_residues
MNSQQSNQLIQGEMLDVIGSIAPQSINLLYLDPPFAAGRVFADSAGAFDDRWQGGLTEYLAWLEQRLVAARRIVAEHGSL
FLHLDRRAVHYAKVLLDQTWGFECFRNEIIWHYTGGGRARRSFSHKHDTILWYSKHPQHWTFNLQAMRQPYKASSGFAKA
GIRSAAGKRYLPHPDGTPVDDVWDIPMLNPMAAERLGYPTQKPERLLERIILAASNSGDLVADLCCGSGTTAAVAQRLGR
CWIAADQSADALALVRGRLRQQAAEWQETLIQPQPASFAAKR

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=257, Percent_Identity=28.0155642023346, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31522; Mature: 31522

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQQSNQLIQGEMLDVIGSIAPQSINLLYLDPPFAAGRVFADSAGAFDDRWQGGLTEYL
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHCCCHHHHH
AWLEQRLVAARRIVAEHGSLFLHLDRRAVHYAKVLLDQTWGFECFRNEIIWHYTGGGRAR
HHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHH
RSFSHKHDTILWYSKHPQHWTFNLQAMRQPYKASSGFAKAGIRSAAGKRYLPHPDGTPVD
HHCCCCCCEEEEEECCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
DVWDIPMLNPMAAERLGYPTQKPERLLERIILAASNSGDLVADLCCGSGTTAAVAQRLGR
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCC
CWIAADQSADALALVRGRLRQQAAEWQETLIQPQPASFAAKR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNSQQSNQLIQGEMLDVIGSIAPQSINLLYLDPPFAAGRVFADSAGAFDDRWQGGLTEYL
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHCCCHHHHH
AWLEQRLVAARRIVAEHGSLFLHLDRRAVHYAKVLLDQTWGFECFRNEIIWHYTGGGRAR
HHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHH
RSFSHKHDTILWYSKHPQHWTFNLQAMRQPYKASSGFAKAGIRSAAGKRYLPHPDGTPVD
HHCCCCCCEEEEEECCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
DVWDIPMLNPMAAERLGYPTQKPERLLERIILAASNSGDLVADLCCGSGTTAAVAQRLGR
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCC
CWIAADQSADALALVRGRLRQQAAEWQETLIQPQPASFAAKR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]