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The map label for this gene is hisH

Identifier: 159896688

GI number: 159896688

Start: 186333

End: 186965

Strand: Direct

Name: hisH

Synonym: Haur_0155

Alternate gene names: 159896688

Gene position: 186333-186965 (Clockwise)

Preceding gene: 159896687

Following gene: 159896689

Centisome position: 2.94

GC content: 53.4

Gene sequence:

>633_bases
ATGATTGCAGTGATCAATTATGGAGCTGGCAACTTGCCCAATGCAGTGCGAGCCTTGGAATATGTTCAAGCCCCGATCGA
AGTGGTGACTGATCCAGCGGCAGTACGGGCGGCGCAGGCAGTGGTTTTGCCTGGCGTTGGCGCAACCGCCGATACCATGC
GCTCGTTGCGTGAAATGGGCATGGATTCAGCAATTCGCGAGGTGATTGCTCGCGGAACGCCATTTTTGGGCATTTGTGTG
GGTATGCAAGTGCTGGCCGAGCAAAGCGAAGAATTTGGCTTGCACGAATGCCTTGGCTTAGTGCCAGGCACGATTCGCCG
CTTCGATCAAGGCTTGAAAGTACCACAAATTGGCTGGAACGGCGTGCAGCACGATGGCAGCGCCTTGTGGGATGGGATTC
CCAACGGAGCTGAATTTTATTTTGTGCATTCCTATTATTTAGCAACTGACGATGTAGCTTTGGTCACTGGCCGTACCGAG
TATGGTCTGAATTTTCCGGCAGCGATTGCCCGCGATAATATTACTGCTGTCCAGTTTCACCCCGAAAAAAGCGGCCAATG
GGGGCTAAAATTGCTGGGGAATTGGGTTGAATTGGCACTGAGTCGGGGTTCAGGGGTCGGGGATCGGGCGTAA

Upstream 100 bases:

>100_bases
TGGCTATCGGAACGTTTTGGAATTTCAAACTAGCCCATAGCCCATCCCAGACCGATCCCCAACCCCCGATCCCCGACCCG
CAATACATACAAGGACACTT

Downstream 100 bases:

>100_bases
CATTCAAGCAATCTCAATATTATAGAGCTATAACAAGGCGATGATAGAGCGCAGCAGGATCGTATAAAACTGGGCTGGTT
TGGCAAACATAGCCGATCCC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIAVINYGAGNLPNAVRALEYVQAPIEVVTDPAAVRAAQAVVLPGVGATADTMRSLREMGMDSAIREVIARGTPFLGICV
GMQVLAEQSEEFGLHECLGLVPGTIRRFDQGLKVPQIGWNGVQHDGSALWDGIPNGAEFYFVHSYYLATDDVALVTGRTE
YGLNFPAAIARDNITAVQFHPEKSGQWGLKLLGNWVELALSRGSGVGDRA

Sequences:

>Translated_210_residues
MIAVINYGAGNLPNAVRALEYVQAPIEVVTDPAAVRAAQAVVLPGVGATADTMRSLREMGMDSAIREVIARGTPFLGICV
GMQVLAEQSEEFGLHECLGLVPGTIRRFDQGLKVPQIGWNGVQHDGSALWDGIPNGAEFYFVHSYYLATDDVALVTGRTE
YGLNFPAAIARDNITAVQFHPEKSGQWGLKLLGNWVELALSRGSGVGDRA
>Mature_210_residues
MIAVINYGAGNLPNAVRALEYVQAPIEVVTDPAAVRAAQAVVLPGVGATADTMRSLREMGMDSAIREVIARGTPFLGICV
GMQVLAEQSEEFGLHECLGLVPGTIRRFDQGLKVPQIGWNGVQHDGSALWDGIPNGAEFYFVHSYYLATDDVALVTGRTE
YGLNFPAAIARDNITAVQFHPEKSGQWGLKLLGNWVELALSRGSGVGDRA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=37.6884422110553, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=32.5358851674641, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_HERA2 (A9B5I2)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001542935.1
- ProteinModelPortal:   A9B5I2
- SMR:   A9B5I2
- GeneID:   5732064
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0155
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   HAUR316274:HAUR_0155-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22466; Mature: 22466

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 180-180 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVINYGAGNLPNAVRALEYVQAPIEVVTDPAAVRAAQAVVLPGVGATADTMRSLREMG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCCCHHHHHHHHHCC
MDSAIREVIARGTPFLGICVGMQVLAEQSEEFGLHECLGLVPGTIRRFDQGLKVPQIGWN
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCC
GVQHDGSALWDGIPNGAEFYFVHSYYLATDDVALVTGRTEYGLNFPAAIARDNITAVQFH
CCCCCCCHHHCCCCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCCHHHHCCCEEEEEEE
PEKSGQWGLKLLGNWVELALSRGSGVGDRA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIAVINYGAGNLPNAVRALEYVQAPIEVVTDPAAVRAAQAVVLPGVGATADTMRSLREMG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCCCHHHHHHHHHCC
MDSAIREVIARGTPFLGICVGMQVLAEQSEEFGLHECLGLVPGTIRRFDQGLKVPQIGWN
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCC
GVQHDGSALWDGIPNGAEFYFVHSYYLATDDVALVTGRTEYGLNFPAAIARDNITAVQFH
CCCCCCCHHHCCCCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCCHHHHCCCEEEEEEE
PEKSGQWGLKLLGNWVELALSRGSGVGDRA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA