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The map label for this gene is ypdA [H]

Identifier: 159896611

GI number: 159896611

Start: 101365

End: 102357

Strand: Reverse

Name: ypdA [H]

Synonym: Haur_0078

Alternate gene names: 159896611

Gene position: 102357-101365 (Counterclockwise)

Preceding gene: 159896612

Following gene: 159896610

Centisome position: 1.61

GC content: 50.96

Gene sequence:

>993_bases
ATGCAATCCTACGATGTGATTATTATTGGGGCTGGGCCAAGTGGCCTGAGCGCGGCGATTGCGGCGCAACAAGCAGGCTT
CAACTATTTGGTGCTTGAGCGTGGCGCAATCACCAACACGATTATTGATTATCCGCTACAAACGGTGTTTTTTAGCACTG
CGCCCAATTTAACGATTGGCAATGTGCCATGGAGCAGTATTCATCCGCATCCAACTCGTACCGAAGCGTTGGCCTACTAT
CGCGGCGTGGTTGAGCATTTTCAATTAAATGTTCAGATGTACGAGCCAGTTCAAGCCTTAGAACGCTTGAGCGATGGCAC
GTTTCAGCTACACACAACCCGCCACGATCAAACCAGTGCTAGGTATGCTGCGCGAGCGGTGATTGTCGCCAGCGGCTCGT
ATGATACGCCCAATCGGTTGGCGATTGCCGGGGCTGAGCTAGGCAAAGTTAAGCATTATTATCGTGAAGGCCACCCCTTT
TATCGCCAAAATGTTGCGGTAATTGGCGGCGGCAATTCAGCTGCCGAAGCTGCGCTCGATTTATATTATCACGGCGCAAA
TGTCACGATCATTCATCAGTTTGCTGGTTTAGATCAACGAATCAAGCCATGGATTGCTGCTGATTTGAAGGCTTGGATCG
AAGCAGGCAAAATTCAGACCTTTTGGGAAGCTAATCTTAGTGCCATTACGCCAACCAGCATCCACATTACCAGCGCCGCT
GGCTCGCAGCACGAACAAGCCAATGATTGGGTTTTTGCCTTGATTGGCTATCGACCAAACACCAGCATGCTCGAAGCAGT
TGGAGTTACGGTTGATCAAGATGCTGTGCCAAGCCATAACTCGACCACCTTTGAAACCAATGTGCCGAATTTATTTATCG
CTGGCGTGTTGGCGGCGGGAGCACACCCGAGCAAAATCTTTATTGAAAATGGCCGTGAGCATGGCCCAGCCATTATCGCC
GAGTTGCGCCAACGTTGGGCAGGGCCGCGCTGA

Upstream 100 bases:

>100_bases
GTTGATGATGTGGGCTTCGATGAGTTAATTGATCCTGTTGGCACCGCAGAGGGCGCTCAATCCTAGCGCTCTCTGCGGTT
TTTTGTTCTGAGGAGCAGTT

Downstream 100 bases:

>100_bases
TGGAGATGGAAGGGTTTCGTTGGTTGTTGAGCGACGCTGGCCAACAACTCTTGGCTGAACTCAGCAATGATCGCGATTTG
AATGAAGCTAACTTTTTGCG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQSYDVIIIGAGPSGLSAAIAAQQAGFNYLVLERGAITNTIIDYPLQTVFFSTAPNLTIGNVPWSSIHPHPTRTEALAYY
RGVVEHFQLNVQMYEPVQALERLSDGTFQLHTTRHDQTSARYAARAVIVASGSYDTPNRLAIAGAELGKVKHYYREGHPF
YRQNVAVIGGGNSAAEAALDLYYHGANVTIIHQFAGLDQRIKPWIAADLKAWIEAGKIQTFWEANLSAITPTSIHITSAA
GSQHEQANDWVFALIGYRPNTSMLEAVGVTVDQDAVPSHNSTTFETNVPNLFIAGVLAAGAHPSKIFIENGREHGPAIIA
ELRQRWAGPR

Sequences:

>Translated_330_residues
MQSYDVIIIGAGPSGLSAAIAAQQAGFNYLVLERGAITNTIIDYPLQTVFFSTAPNLTIGNVPWSSIHPHPTRTEALAYY
RGVVEHFQLNVQMYEPVQALERLSDGTFQLHTTRHDQTSARYAARAVIVASGSYDTPNRLAIAGAELGKVKHYYREGHPF
YRQNVAVIGGGNSAAEAALDLYYHGANVTIIHQFAGLDQRIKPWIAADLKAWIEAGKIQTFWEANLSAITPTSIHITSAA
GSQHEQANDWVFALIGYRPNTSMLEAVGVTVDQDAVPSHNSTTFETNVPNLFIAGVLAAGAHPSKIFIENGREHGPAIIA
ELRQRWAGPR
>Mature_330_residues
MQSYDVIIIGAGPSGLSAAIAAQQAGFNYLVLERGAITNTIIDYPLQTVFFSTAPNLTIGNVPWSSIHPHPTRTEALAYY
RGVVEHFQLNVQMYEPVQALERLSDGTFQLHTTRHDQTSARYAARAVIVASGSYDTPNRLAIAGAELGKVKHYYREGHPF
YRQNVAVIGGGNSAAEAALDLYYHGANVTIIHQFAGLDQRIKPWIAADLKAWIEAGKIQTFWEANLSAITPTSIHITSAA
GSQHEQANDWVFALIGYRPNTSMLEAVGVTVDQDAVPSHNSTTFETNVPNLFIAGVLAAGAHPSKIFIENGREHGPAIIA
ELRQRWAGPR

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 35942; Mature: 35942

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYDVIIIGAGPSGLSAAIAAQQAGFNYLVLERGAITNTIIDYPLQTVFFSTAPNLTIG
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHCCHHEEEEECCCCCEEC
NVPWSSIHPHPTRTEALAYYRGVVEHFQLNVQMYEPVQALERLSDGTFQLHTTRHDQTSA
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECHHHHHHHCCCCCEEEEECCCCCHHH
RYAARAVIVASGSYDTPNRLAIAGAELGKVKHYYREGHPFYRQNVAVIGGGNSAAEAALD
HHEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHCCEEEEECCCHHHHHEEE
LYYHGANVTIIHQFAGLDQRIKPWIAADLKAWIEAGKIQTFWEANLSAITPTSIHITSAA
EEEECCCEEEEEECCCCCHHHCHHHHHHHHHHHHCCCEEEEEECCCCEECCCEEEEEECC
GSQHEQANDWVFALIGYRPNTSMLEAVGVTVDQDAVPSHNSTTFETNVPNLFIAGVLAAG
CCCCCCCCCEEEEEEEECCCCHHHHHHCCEECCCCCCCCCCEEEECCCCCHHHHHHHHCC
AHPSKIFIENGREHGPAIIAELRQRWAGPR
CCCCEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSYDVIIIGAGPSGLSAAIAAQQAGFNYLVLERGAITNTIIDYPLQTVFFSTAPNLTIG
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHCCHHEEEEECCCCCEEC
NVPWSSIHPHPTRTEALAYYRGVVEHFQLNVQMYEPVQALERLSDGTFQLHTTRHDQTSA
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECHHHHHHHCCCCCEEEEECCCCCHHH
RYAARAVIVASGSYDTPNRLAIAGAELGKVKHYYREGHPFYRQNVAVIGGGNSAAEAALD
HHEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHCCEEEEECCCHHHHHEEE
LYYHGANVTIIHQFAGLDQRIKPWIAADLKAWIEAGKIQTFWEANLSAITPTSIHITSAA
EEEECCCEEEEEECCCCCHHHCHHHHHHHHHHHHCCCEEEEEECCCCEECCCEEEEEECC
GSQHEQANDWVFALIGYRPNTSMLEAVGVTVDQDAVPSHNSTTFETNVPNLFIAGVLAAG
CCCCCCCCCEEEEEEEECCCCHHHHHHCCEECCCCCCCCCCEEEECCCCCHHHHHHHHCC
AHPSKIFIENGREHGPAIIAELRQRWAGPR
CCCCEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]