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The map label for this gene is repB [H]

Identifier: 15925734

GI number: 15925734

Start: 37410

End: 38204

Strand: Reverse

Name: repB [H]

Synonym: SA0028

Alternate gene names: 15925734

Gene position: 38204-37410 (Counterclockwise)

Preceding gene: 15925736

Following gene: 15925733

Centisome position: 1.36

GC content: 32.58

Gene sequence:

>795_bases
ATGGGGGTTTCTTTTAATATTATGTGTCCTAATAGTAGCATTTATTCAGATGAAAAATCAAGGGTTTTAGTGGACAAGAC
AAAAAGTGGAAAAGTGAGACCATGGAGAGAAAAGAAAATCGCTAATGTTGATTACTTTGAACTTCTGCATATTCTTGAAT
TTAAAAAGGCTGAAAGAGTAAAAGATTGTGCTGAAATATTAGAGTATAAACAAAATCGTGAAACAGGCGAAAGAAAGTTG
TATCGAGTGTGGTTTTGTAAATCCAGGCTTTGTCCAATGTGCAACTGGAGGAGAGCAATGAAACATGGCATTCAGTCACA
AAAGGTTGTTGCTGAAGTTATTAAACAAAAGCCAACAGTTCGTTGGTTGTTTCTCACATTAACAGTTAAAAATGTTTATG
ATGGCGAAGAATTAAATAAGAGTTTGTCAGATATGGCTCAAGGATTTCGCCGAATGATGCAATATAAAAAAATTAATAAA
AATCTTGTTGGTTTTATGCGTGCAACGGAAGTGACAATAAATAATAAAGATAATTCTTATAATCAGCACATGCATGTATT
GGTATGTGCGGAACCAACTTATTTTAAGAATACAGAAAACTGCGTGAATCAAAAACAATGGATTCAATTTTGGAAAAAGG
CAATGAAATTAGACTATGATCCAAATGTAAAAGTTCAAATGATTCGACCGAAAAATAAATATAAATCGGATATACAATCG
GCAATTGACGAAACTGCAAAATATCCTGTAAAGGATACGGATTTTATGACCGATGATGAAGAAAAGAATTTGTAA

Upstream 100 bases:

>100_bases
GGTATGTATGTGTTTTAAAGTATTGAAAACCCTTAAAATTGGTTGCACAGAAAAACCCCATCTGTTAAAGTTATAAGTGA
CTAAACAAATAACTAAATAG

Downstream 100 bases:

>100_bases
CGTTTGTCTGATTTGGAGGAAGGTTTACACCGTAAAAGGTTAATCTCCTATGGTGGTTTGTTAAAAGAAATACATAAAAA
ATTAAACCTTGATGACACAG

Product: truncated replication protein for pUB110 plasmid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERVKDCAEILEYKQNRETGERKL
YRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
NLVGFMRATEVTINNKDNSYNQHMHVLVCAEPTYFKNTENCVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQS
AIDETAKYPVKDTDFMTDDEEKNL

Sequences:

>Translated_264_residues
MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERVKDCAEILEYKQNRETGERKL
YRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
NLVGFMRATEVTINNKDNSYNQHMHVLVCAEPTYFKNTENCVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQS
AIDETAKYPVKDTDFMTDDEEKNL
>Mature_263_residues
GVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERVKDCAEILEYKQNRETGERKLY
RVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKN
LVGFMRATEVTINNKDNSYNQHMHVLVCAEPTYFKNTENCVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSA
IDETAKYPVKDTDFMTDDEEKNL

Specific function: Produces a single-strand nick in a specific site of the plasmid, and this nick results in single-strand replication by rolling circle mechanism [H]

COG id: COG5655

COG function: function code L; Plasmid rolling circle replication initiator protein and truncated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-positive plasmids replication protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000989 [H]

Pfam domain/function: PF01446 Rep_1 [H]

EC number: NA

Molecular weight: Translated: 31371; Mature: 31240

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERV
CCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
KDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTV
HHHHHHHHHHHCCCHHHHHHHHHEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCE
RWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATEVTINNKDNSY
EEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
NQHMHVLVCAEPTYFKNTENCVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQS
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
AIDETAKYPVKDTDFMTDDEEKNL
HHHHHHCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
GVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERV
CCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
KDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTV
HHHHHHHHHHHCCCHHHHHHHHHEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCE
RWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATEVTINNKDNSY
EEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
NQHMHVLVCAEPTYFKNTENCVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQS
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
AIDETAKYPVKDTDFMTDDEEKNL
HHHHHHCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3007933; 2677995 [H]