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The map label for this gene is repB [H]

Identifier: 15925733

GI number: 15925733

Start: 37200

End: 37406

Strand: Reverse

Name: repB [H]

Synonym: SA0027

Alternate gene names: 15925733

Gene position: 37406-37200 (Counterclockwise)

Preceding gene: 15925734

Following gene: 15925732

Centisome position: 1.33

GC content: 32.85

Gene sequence:

>207_bases
TTGTCTGATTTGGAGGAAGGTTTACACCGTAAAAGGTTAATCTCCTATGGTGGTTTGTTAAAAGAAATACATAAAAAATT
AAACCTTGATGACACAGAAGAAGGCGATTTGATTCATACAGATGATGACGAAAAAGCCGATGAAGATGGATTTTCTATTA
TTGCAATGTGGAATTGGGAACGGAAAAATTATTTTATTAAAGAGTAG

Upstream 100 bases:

>100_bases
ATATAAATCGGATATACAATCGGCAATTGACGAAACTGCAAAATATCCTGTAAAGGATACGGATTTTATGACCGATGATG
AAGAAAAGAATTTGTAACGT

Downstream 100 bases:

>100_bases
TTCAACAAACGGGCCAGTTTGTTGAAGATTAGATGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGG
AGTCTTCTGTATGAACTATT

Product: truncated replication protein for plasmid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE

Sequences:

>Translated_68_residues
MSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
>Mature_67_residues
SDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE

Specific function: Produces a single-strand nick in a specific site of the plasmid, and this nick results in single-strand replication by rolling circle mechanism [H]

COG id: COG5655

COG function: function code L; Plasmid rolling circle replication initiator protein and truncated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-positive plasmids replication protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000989 [H]

Pfam domain/function: PF01446 Rep_1 [H]

EC number: NA

Molecular weight: Translated: 8066; Mature: 7935

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCC
RKNYFIKE
CCCEECCC
>Mature Secondary Structure 
SDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCC
RKNYFIKE
CCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3007933; 2677995 [H]