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The map label for this gene is ilvI [H]

Identifier: 15922741

GI number: 15922741

Start: 2413273

End: 2414868

Strand: Direct

Name: ilvI [H]

Synonym: ST2410

Alternate gene names: 15922741

Gene position: 2413273-2414868 (Clockwise)

Preceding gene: 15922740

Following gene: 15922750

Centisome position: 89.55

GC content: 36.59

Gene sequence:

>1596_bases
ATGGATAGCGGTGCAAGACTCATTTTAAAAAGTCTACAAGATTTAGGTGTAGATAAAATCTTTATGGTATCTGGAACAGA
TTATGCAGCATTTATTGAAGAAAAAGTAAGGGATCCTTCATTGCCAGATTTCGTAATAGTACCACATGAAATAACAGCTA
CTGCTGCAGCTATAGGCTATTCTCTATCTGGAAAAATAGGGGTTGTTGCAGTTCATACAATTCCGGGTACTACAAATGCT
TTAGGAATGATAATTAATGCTTTCACATCTAGAATACCCCTAATAGTTATTGCTGGCAGAAGTCCTTATACTGAAACTGG
TAGTCCAGCAAGTAGGAATTTGAGAATACATTGGACTCAAGAGGCTAGGGATCAAGGAGAAATTGTTAGGCAATGGGTTA
AATATGATTTTGAAATAAGGAGGACTGAGCAAATACCAGAGGTTATAGCTAGGGCTTATCAGATTGCATTTAGTGAGCCT
AAGGGACCAGTATATATTGTGATTCCTAGAGAGGTGAGTATAGAGAAAAGCGAGTACAGAAAAGTTAGAGTTTCAACTTT
TGAGCCAGGAATGAAAAGAGAAGATCTAGTAAAGGCTAAGAAAATGATCGAGGAGAGTGAAAGACCAATTATAATTACTT
GGAGGGGTGGTAGAAGGAAAGAGTGGTTTGATAGCATAAAAACGTTTGCTGATAAAGTAGGGATACCAGTTTTAAATTAC
ATAGGAGAGGTTGTAAATTATTCTGGTGAAATGGGATTAGATAGGATTGATTTATCAACTGTTGATTTAGCTATTGTAGT
AGAGGCCGAGGTACCTTATATTCCAAAGAAAACTAAATTTGATGGCAAAATTATAAAGGTTGATGTAGATCCATCTTACT
CTTATATCCCATTTTATGGTTTTCCTTGCGACTTGTGTATCCAATCTACCGTTTCAGAATTCTTTGACCAACTAGATGTT
AAGGAGAAAAAGGAGTGGAAGGAAAAAGTAAAAGAGCTGAGGCTTCAACAAGAAAAGAAAAAGGAAGAGGAAATAGAACA
ATTGAGGAAAAGGAGGTCAATTCATCCTAGATATTTAAGTTACGTTATTGGTAAGGTAATTGGGAAAGATGATGTTATAC
TTAATGAGTATCCATTTAATCCTAGATATACAAAGCTTGATTTTGGGCAATACTTCGCTGATCCTTCATTTGGTCATTTA
GGCTGGGCTTTAGGTGCTTCTTTCGGTGTCTCGTTAACAAATAGGAAAGTTATTGCTACAGTTGGTGATGGTGCATTTAT
TTTTGGAGTGCCAGAGGCATTTTATTATGCAATTTCCACATATGGTGGTAATGTCACGGTAATAATTTATGATAATGGTG
GCTGGCTAGCTAGTGCTGAAGCTGTTGAAGAAGTTTTTCCAGAAGGATTGGCAAAGGCAAAGCAGTATTTTCCTGGAGCA
GACTTTAAGAGGTATAATATTGGCGAAACCGCTAAGGTTTTTGGTGGGTATTATAGGTTGGTGGAAGATGTTAATGAAGT
AGAAGAGAGTGTAAGAGAAGCATACAACTTTAGAGGTATTTCTGTACTGCAAGTTATTGTGGATAGAGTAAGATAA

Upstream 100 bases:

>100_bases
TTCTTCCTATTTTCTCCATTATATCTTTGGGCATTAATTTCTATTTTAGGCTTTCTCTCTATTCCATTATATTTATTAAG
TATCAAGAGGATTGAAACAA

Downstream 100 bases:

>100_bases
AACTATTATTTATTTGCCTCTCTAATTTGTTTTAATTCCCTTTCATATGCTTCTACGATAGAATCGTATGTCAGTATCCC
TTTAAATTTTCCTTTTTCTA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MDSGARLILKSLQDLGVDKIFMVSGTDYAAFIEEKVRDPSLPDFVIVPHEITATAAAIGYSLSGKIGVVAVHTIPGTTNA
LGMIINAFTSRIPLIVIAGRSPYTETGSPASRNLRIHWTQEARDQGEIVRQWVKYDFEIRRTEQIPEVIARAYQIAFSEP
KGPVYIVIPREVSIEKSEYRKVRVSTFEPGMKREDLVKAKKMIEESERPIIITWRGGRRKEWFDSIKTFADKVGIPVLNY
IGEVVNYSGEMGLDRIDLSTVDLAIVVEAEVPYIPKKTKFDGKIIKVDVDPSYSYIPFYGFPCDLCIQSTVSEFFDQLDV
KEKKEWKEKVKELRLQQEKKKEEEIEQLRKRRSIHPRYLSYVIGKVIGKDDVILNEYPFNPRYTKLDFGQYFADPSFGHL
GWALGASFGVSLTNRKVIATVGDGAFIFGVPEAFYYAISTYGGNVTVIIYDNGGWLASAEAVEEVFPEGLAKAKQYFPGA
DFKRYNIGETAKVFGGYYRLVEDVNEVEESVREAYNFRGISVLQVIVDRVR

Sequences:

>Translated_531_residues
MDSGARLILKSLQDLGVDKIFMVSGTDYAAFIEEKVRDPSLPDFVIVPHEITATAAAIGYSLSGKIGVVAVHTIPGTTNA
LGMIINAFTSRIPLIVIAGRSPYTETGSPASRNLRIHWTQEARDQGEIVRQWVKYDFEIRRTEQIPEVIARAYQIAFSEP
KGPVYIVIPREVSIEKSEYRKVRVSTFEPGMKREDLVKAKKMIEESERPIIITWRGGRRKEWFDSIKTFADKVGIPVLNY
IGEVVNYSGEMGLDRIDLSTVDLAIVVEAEVPYIPKKTKFDGKIIKVDVDPSYSYIPFYGFPCDLCIQSTVSEFFDQLDV
KEKKEWKEKVKELRLQQEKKKEEEIEQLRKRRSIHPRYLSYVIGKVIGKDDVILNEYPFNPRYTKLDFGQYFADPSFGHL
GWALGASFGVSLTNRKVIATVGDGAFIFGVPEAFYYAISTYGGNVTVIIYDNGGWLASAEAVEEVFPEGLAKAKQYFPGA
DFKRYNIGETAKVFGGYYRLVEDVNEVEESVREAYNFRGISVLQVIVDRVR
>Mature_531_residues
MDSGARLILKSLQDLGVDKIFMVSGTDYAAFIEEKVRDPSLPDFVIVPHEITATAAAIGYSLSGKIGVVAVHTIPGTTNA
LGMIINAFTSRIPLIVIAGRSPYTETGSPASRNLRIHWTQEARDQGEIVRQWVKYDFEIRRTEQIPEVIARAYQIAFSEP
KGPVYIVIPREVSIEKSEYRKVRVSTFEPGMKREDLVKAKKMIEESERPIIITWRGGRRKEWFDSIKTFADKVGIPVLNY
IGEVVNYSGEMGLDRIDLSTVDLAIVVEAEVPYIPKKTKFDGKIIKVDVDPSYSYIPFYGFPCDLCIQSTVSEFFDQLDV
KEKKEWKEKVKELRLQQEKKKEEEIEQLRKRRSIHPRYLSYVIGKVIGKDDVILNEYPFNPRYTKLDFGQYFADPSFGHL
GWALGASFGVSLTNRKVIATVGDGAFIFGVPEAFYYAISTYGGNVTVIIYDNGGWLASAEAVEEVFPEGLAKAKQYFPGA
DFKRYNIGETAKVFGGYYRLVEDVNEVEESVREAYNFRGISVLQVIVDRVR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=22.8621291448517, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081685, Length=484, Percent_Identity=23.3471074380165, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=23.4767025089606, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=23.0496453900709, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788716, Length=498, Percent_Identity=24.0963855421687, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786717, Length=432, Percent_Identity=21.9907407407407, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=242, Percent_Identity=26.8595041322314, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=22.5577264653641, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60089; Mature: 60089

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGARLILKSLQDLGVDKIFMVSGTDYAAFIEEKVRDPSLPDFVIVPHEITATAAAIGY
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCE
SLSGKIGVVAVHTIPGTTNALGMIINAFTSRIPLIVIAGRSPYTETGSPASRNLRIHWTQ
EECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECH
EARDQGEIVRQWVKYDFEIRRTEQIPEVIARAYQIAFSEPKGPVYIVIPREVSIEKSEYR
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHCC
KVRVSTFEPGMKREDLVKAKKMIEESERPIIITWRGGRRKEWFDSIKTFADKVGIPVLNY
EEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHH
IGEVVNYSGEMGLDRIDLSTVDLAIVVEAEVPYIPKKTKFDGKIIKVDVDPSYSYIPFYG
HHHHHCCCCCCCCCEECCCEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEC
FPCDLCIQSTVSEFFDQLDVKEKKEWKEKVKELRLQQEKKKEEEIEQLRKRRSIHPRYLS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YVIGKVIGKDDVILNEYPFNPRYTKLDFGQYFADPSFGHLGWALGASFGVSLTNRKVIAT
HHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCEECCCEEEEE
VGDGAFIFGVPEAFYYAISTYGGNVTVIIYDNGGWLASAEAVEEVFPEGLAKAKQYFPGA
ECCCEEEEECHHHHHEEHEECCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
DFKRYNIGETAKVFGGYYRLVEDVNEVEESVREAYNFRGISVLQVIVDRVR
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDSGARLILKSLQDLGVDKIFMVSGTDYAAFIEEKVRDPSLPDFVIVPHEITATAAAIGY
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCE
SLSGKIGVVAVHTIPGTTNALGMIINAFTSRIPLIVIAGRSPYTETGSPASRNLRIHWTQ
EECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECH
EARDQGEIVRQWVKYDFEIRRTEQIPEVIARAYQIAFSEPKGPVYIVIPREVSIEKSEYR
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHCC
KVRVSTFEPGMKREDLVKAKKMIEESERPIIITWRGGRRKEWFDSIKTFADKVGIPVLNY
EEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHH
IGEVVNYSGEMGLDRIDLSTVDLAIVVEAEVPYIPKKTKFDGKIIKVDVDPSYSYIPFYG
HHHHHCCCCCCCCCEECCCEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEC
FPCDLCIQSTVSEFFDQLDVKEKKEWKEKVKELRLQQEKKKEEEIEQLRKRRSIHPRYLS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YVIGKVIGKDDVILNEYPFNPRYTKLDFGQYFADPSFGHLGWALGASFGVSLTNRKVIAT
HHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCEECCCEEEEE
VGDGAFIFGVPEAFYYAISTYGGNVTVIIYDNGGWLASAEAVEEVFPEGLAKAKQYFPGA
ECCCEEEEECHHHHHEEHEECCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
DFKRYNIGETAKVFGGYYRLVEDVNEVEESVREAYNFRGISVLQVIVDRVR
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]