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The map label for this gene is nuoI [H]

Identifier: 15922548

GI number: 15922548

Start: 2227176

End: 2227685

Strand: Direct

Name: nuoI [H]

Synonym: ST2220

Alternate gene names: 15922548

Gene position: 2227176-2227685 (Clockwise)

Preceding gene: 15922547

Following gene: 15922549

Centisome position: 82.65

GC content: 34.12

Gene sequence:

>510_bases
ATGGTTATGGATAAAGTAAAAGAATATAAAAAAGAAAAAAATCCGTTTAGATTATTTGTAGATCATTTAAATGCAGTAGG
AACTGGAATAAAGTATATGATACAACCTCAAAGAATAACGTTAAAATATCCAGAAGAATCGCTAACATTACCAACTGGTT
ATAGAGGAATGATAAGGTTATACAAAGATATTTGTATAGGGTGTACTTTATGTGCATTAATTTGTCCAGCAGACGCAATG
AAAATGGTAACTGAGGGAGGAAAGAAGTTTCCCTCAATTAACTATGGTAGGTGTGTATTCTGTGGTTTTTGTGTTGATGT
GTGTCCAGTTGACGCCTTAAAAGAAACTAGGGTTCATGATGCTGTATTTACCAATAGGAGAGATTTAGTATTTAGACCAG
ATAGGTTCGATCAAGATTTTGATCAACCGATACCTTCTCAAGGTATAGTGAAAAAAGTTAGAGCTGTTGTTGACGAAAAG
AAGGGGATCAAATATGTCCCTGATGAGTGA

Upstream 100 bases:

>100_bases
GTGTTTATGGCAGATATAGGATTGATCAAGCCTTAAGGGGTTCTTGGAAATATTTACTTCCCGTAGCTTTTGCCTCTTTA
GTTTTAGGTTTGGTGGTGGG

Downstream 100 bases:

>100_bases
TTTTCAAGTTTCATTTTTTGTATTTTTTGGAATAATAAGTATTGCTTCTGCACTGTTTATAACTAGGCAAAAGAACGTAT
TTTATGCAGCGATAGGTCTA

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MVMDKVKEYKKEKNPFRLFVDHLNAVGTGIKYMIQPQRITLKYPEESLTLPTGYRGMIRLYKDICIGCTLCALICPADAM
KMVTEGGKKFPSINYGRCVFCGFCVDVCPVDALKETRVHDAVFTNRRDLVFRPDRFDQDFDQPIPSQGIVKKVRAVVDEK
KGIKYVPDE

Sequences:

>Translated_169_residues
MVMDKVKEYKKEKNPFRLFVDHLNAVGTGIKYMIQPQRITLKYPEESLTLPTGYRGMIRLYKDICIGCTLCALICPADAM
KMVTEGGKKFPSINYGRCVFCGFCVDVCPVDALKETRVHDAVFTNRRDLVFRPDRFDQDFDQPIPSQGIVKKVRAVVDEK
KGIKYVPDE
>Mature_169_residues
MVMDKVKEYKKEKNPFRLFVDHLNAVGTGIKYMIQPQRITLKYPEESLTLPTGYRGMIRLYKDICIGCTLCALICPADAM
KMVTEGGKKFPSINYGRCVFCGFCVDVCPVDALKETRVHDAVFTNRRDLVFRPDRFDQDFDQPIPSQGIVKKVRAVVDEK
KGIKYVPDE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=145, Percent_Identity=28.9655172413793, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788617, Length=106, Percent_Identity=38.6792452830189, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI1789075, Length=111, Percent_Identity=31.5315315315315, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=130, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17864306, Length=130, Percent_Identity=29.2307692307692, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19335; Mature: 19335

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMDKVKEYKKEKNPFRLFVDHLNAVGTGIKYMIQPQRITLKYPEESLTLPTGYRGMIRL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHH
YKDICIGCTLCALICPADAMKMVTEGGKKFPSINYGRCVFCGFCVDVCPVDALKETRVHD
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AVFTNRRDLVFRPDRFDQDFDQPIPSQGIVKKVRAVVDEKKGIKYVPDE
HHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVMDKVKEYKKEKNPFRLFVDHLNAVGTGIKYMIQPQRITLKYPEESLTLPTGYRGMIRL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHH
YKDICIGCTLCALICPADAMKMVTEGGKKFPSINYGRCVFCGFCVDVCPVDALKETRVHD
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AVFTNRRDLVFRPDRFDQDFDQPIPSQGIVKKVRAVVDEKKGIKYVPDE
HHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA