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The map label for this gene is hisF

Identifier: 15921755

GI number: 15921755

Start: 1466272

End: 1467021

Strand: Direct

Name: hisF

Synonym: ST1462

Alternate gene names: 15921755

Gene position: 1466272-1467021 (Clockwise)

Preceding gene: 15921754

Following gene: 15921756

Centisome position: 54.41

GC content: 35.87

Gene sequence:

>750_bases
ATGACAGCTAAAAGAATAATTGCTTGTTTAGATGTAAAAAACGGTAGAGTAGTCAAGGGTGTAAATTTCCTTAACTTAAA
AGACAAGGGAGACCCCGTAGAATTAGCATCTAGATACGAAGAAGAAGGAGCGGACGAAATAGTATTTTTGGATATTACAG
CTACTATAGAAGGAAGAAAAGCATTATTAGAAGTAGTAAAAAATACGGCTAGCGTATTATCTATACCACTGACGGTAGGA
GGTGGAATAAGAACAATAGAAGATGTTTCAAGAATTCTAGGTAATGGCGCTGATAAAGTAAGTATAAATACAGCAGCAGT
TGAAAACAAAAAGATAATAACTGAGGCCTCTGAACAATTTGGTGCTCAAGCAATTGTTGTAGCAATTGATGTGAAAAGGG
TTAATAATTCGTTCATAGTTTTTACTAGATCTGGTACCTATAATACCGGTATAGACGCAATTCAATGGGCAAAAGAAGTA
GAAAAATTGGGTGCTGGGGAAATTTTGCTTACAAGTATTGATAAAGATGGTACAAGAGAAGGATATGATATCGAGCTTAC
AAAAGAGGTAAATAATTCTGTAAATATTCCAGTTATTGCTAGTGGTGGTGCAGGGAAAATGGAACATTTTTATGAGGTTT
TAAAAGTTGCGGATGCCGCGTTGGCTGCAGGTGTTTTTCATGATGGCGTTATTAAGATCCCTGAACTTAAAAGGTTTTTG
TTAGAGAAGGGTATAGAGGTGAGAGTATGA

Upstream 100 bases:

>100_bases
CTCATCATGTTATCGAAGCATCGTTTAAGGGTCTTGGTTTTTCGTTGTATGAAGCTTCCAGAATAGTTTATGAAGAAACT
TACAGTCTAAAGGGATCTTT

Downstream 100 bases:

>100_bases
TATTAAAGGAGTTACCTAAGCAAAGACCTAATGATTTTACCAAAGTACTTCAAGATGTAGAGAAGATTATTAGTTATGTT
AAGCAAAAAGGTGACGAGGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTAKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELASRYEEEGADEIVFLDITATIEGRKALLEVVKNTASVLSIPLTVG
GGIRTIEDVSRILGNGADKVSINTAAVENKKIITEASEQFGAQAIVVAIDVKRVNNSFIVFTRSGTYNTGIDAIQWAKEV
EKLGAGEILLTSIDKDGTREGYDIELTKEVNNSVNIPVIASGGAGKMEHFYEVLKVADAALAAGVFHDGVIKIPELKRFL
LEKGIEVRV

Sequences:

>Translated_249_residues
MTAKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELASRYEEEGADEIVFLDITATIEGRKALLEVVKNTASVLSIPLTVG
GGIRTIEDVSRILGNGADKVSINTAAVENKKIITEASEQFGAQAIVVAIDVKRVNNSFIVFTRSGTYNTGIDAIQWAKEV
EKLGAGEILLTSIDKDGTREGYDIELTKEVNNSVNIPVIASGGAGKMEHFYEVLKVADAALAAGVFHDGVIKIPELKRFL
LEKGIEVRV
>Mature_248_residues
TAKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELASRYEEEGADEIVFLDITATIEGRKALLEVVKNTASVLSIPLTVGG
GIRTIEDVSRILGNGADKVSINTAAVENKKIITEASEQFGAQAIVVAIDVKRVNNSFIVFTRSGTYNTGIDAIQWAKEVE
KLGAGEILLTSIDKDGTREGYDIELTKEVNNSVNIPVIASGGAGKMEHFYEVLKVADAALAAGVFHDGVIKIPELKRFLL
EKGIEVRV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=48.4496124031008, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=30, Blast_Score=88, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=38.0191693290735, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SULTO (Q970Z0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377424.1
- ProteinModelPortal:   Q970Z0
- SMR:   Q970Z0
- GeneID:   1459497
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1462
- NMPDR:   fig|273063.1.peg.1592
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   STOK273063:ST1462-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26810; Mature: 26679

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELASRYEEEGADEIVFLDITATIEGRK
CCHHHEEEEEECCCCEEEECEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECHHH
ALLEVVKNTASVLSIPLTVGGGIRTIEDVSRILGNGADKVSINTAAVENKKIITEASEQF
HHHHHHHCCHHHEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHC
GAQAIVVAIDVKRVNNSFIVFTRSGTYNTGIDAIQWAKEVEKLGAGEILLTSIDKDGTRE
CCEEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIELTKEVNNSVNIPVIASGGAGKMEHFYEVLKVADAALAAGVFHDGVIKIPELKRFL
CCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
LEKGIEVRV
HHCCCEECC
>Mature Secondary Structure 
TAKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELASRYEEEGADEIVFLDITATIEGRK
CHHHEEEEEECCCCEEEECEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECHHH
ALLEVVKNTASVLSIPLTVGGGIRTIEDVSRILGNGADKVSINTAAVENKKIITEASEQF
HHHHHHHCCHHHEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHC
GAQAIVVAIDVKRVNNSFIVFTRSGTYNTGIDAIQWAKEVEKLGAGEILLTSIDKDGTRE
CCEEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIELTKEVNNSVNIPVIASGGAGKMEHFYEVLKVADAALAAGVFHDGVIKIPELKRFL
CCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
LEKGIEVRV
HHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479