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The map label for this gene is gatA

Identifier: 15921546

GI number: 15921546

Start: 1278307

End: 1279737

Strand: Reverse

Name: gatA

Synonym: ST1283

Alternate gene names: 15921546

Gene position: 1279737-1278307 (Counterclockwise)

Preceding gene: 15921548

Following gene: 15921545

Centisome position: 47.49

GC content: 34.52

Gene sequence:

>1431_bases
ATGGGGAGTAATATGATTATGAAACTGGTAGAAGACTTACTAAATAAGAACTTAGATATTGATGAATATGTTGAAAGAAC
ATATGAAAGAATCGAGAAAATTGAGAAAAAAATCAAAGCGTTTATAACAATAAGGGATAAAGAAGAAGTTAAGAGAGAAG
TAAGGGAAAAGCTTAAGAATCCTAAAGGGAAATTATCTGGAATTCTAATAGCTATTAAGGATAACATCTCCACAAAAGGA
ATTAGAACCACTTGTGCTTCTAGAATGTTAGAGGATTATGTACCGCCATATGATGCCACTGTAATAGAAAAATTAAAGCA
AGAAGGAGCAGTTATTCTAGGCAAAACAAATATGGATGAATTTGCAATGGGATCGACAACGGAGACAAGTTATTTTGGCC
CTACAAGAAATCCTTGGGACCTAGAGAGAACACCTGGGGGAAGCTCTGGGGGAAGCGGAGCCGCATTAGCTGCAGGAATA
GTAGATATAGCTTTAGGTAGTGATACTGGAGGTTCAATAAGAGCGCCAGCTGCGTATAATGCCGTATTTGGCTTAAAGCC
TTCTTATGGGACAGTTAGTAGATTTGGGTTAGTAGCTTATGCAAATAGTTTAGAACAAATAGGACCGATGGCAAAAAATG
CTGAAGATCTGGCTTTACTCTATTCAATAATCTCTGGTGATGACCCTAAAGACGCTACTACAATTCATTTTGAACCCCAA
GTACCGGAAAAAGTAGAACTGAAAGATGTTAAAATTGCTGTTTTGAAGGATATAGTAGAGGCATCAGATAAACCAGTTGT
TTCAATATTTAACTCAACTCTAGATAAATTAAGTTCTGAGGGGGCAATAATTAAAGAAGTGAATTTAGGATATGCTGAAT
ATGCTTTACCGGCTTATTATATTATTGCTATGTCAGAGGCTAGCTCTAATTTAGCTAGATTTGATGGTGTAAGATACGGA
TATAGTAAATACTCTGAAGGTAATTGGAGAGAGACTTTTGCTAAAAATAGAGGAGAAGGATTTGGAATAGAAGTTAAAAG
AAGAATATTATTAGGAAGCTTTATCCTAAGTGCAGGATATTATGAAGAGTTCTATATTAAAGCACTTAAAATTAGGAGAT
TAATAAAAGATAATGTTGATAATATACTTAAAGAATTCGATCTAATAGCATCACCAACTATGCCTATATTGCCTCCAAAA
ATAGGTGAGGTAGTTGAGGATCCTATTAAAATGTATGCTATGGATCTAAACACTGTAATTGCAAATTTAGCTGGAGTACC
AGCTTTATCTCAGCCAGCAGGATTCTATAATAATTTACCCATAGGATTACAACTTATGGGAAGATATTTGTCGGACTATT
ACATTATGGCTATTTCAGCAAAAATAGAAAAAGTACTTAACCTTTATGATTTAACAGCACCTATAAGTTAA

Upstream 100 bases:

>100_bases
AAGCTTAGAAAAGATGAAATTATAAAACCATTGAGTAGAGATGAAGCATTAAGTAATGTGAAAAGAAAGGAGGATGGCTT
TATAGTAGGACCTGCTACCT

Downstream 100 bases:

>100_bases
AGTTTTTAATCTTACATTCAGTAGTTTAATAGATGGTAAGTAATCTTAGGATAGATACTTTAAGCTTGATAATGAAAGAA
CTAGAAAAAGAAAAATATCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGSNMIMKLVEDLLNKNLDIDEYVERTYERIEKIEKKIKAFITIRDKEEVKREVREKLKNPKGKLSGILIAIKDNISTKG
IRTTCASRMLEDYVPPYDATVIEKLKQEGAVILGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGI
VDIALGSDTGGSIRAPAAYNAVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQ
VPEKVELKDVKIAVLKDIVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLGYAEYALPAYYIIAMSEASSNLARFDGVRYG
YSKYSEGNWRETFAKNRGEGFGIEVKRRILLGSFILSAGYYEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPK
IGEVVEDPIKMYAMDLNTVIANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIMAISAKIEKVLNLYDLTAPIS

Sequences:

>Translated_476_residues
MGSNMIMKLVEDLLNKNLDIDEYVERTYERIEKIEKKIKAFITIRDKEEVKREVREKLKNPKGKLSGILIAIKDNISTKG
IRTTCASRMLEDYVPPYDATVIEKLKQEGAVILGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGI
VDIALGSDTGGSIRAPAAYNAVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQ
VPEKVELKDVKIAVLKDIVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLGYAEYALPAYYIIAMSEASSNLARFDGVRYG
YSKYSEGNWRETFAKNRGEGFGIEVKRRILLGSFILSAGYYEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPK
IGEVVEDPIKMYAMDLNTVIANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIMAISAKIEKVLNLYDLTAPIS
>Mature_475_residues
GSNMIMKLVEDLLNKNLDIDEYVERTYERIEKIEKKIKAFITIRDKEEVKREVREKLKNPKGKLSGILIAIKDNISTKGI
RTTCASRMLEDYVPPYDATVIEKLKQEGAVILGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGIV
DIALGSDTGGSIRAPAAYNAVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQV
PEKVELKDVKIAVLKDIVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLGYAEYALPAYYIIAMSEASSNLARFDGVRYGY
SKYSEGNWRETFAKNRGEGFGIEVKRRILLGSFILSAGYYEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPKI
GEVVEDPIKMYAMDLNTVIANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIMAISAKIEKVLNLYDLTAPIS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=477, Percent_Identity=37.1069182389937, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=25.3086419753086, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI166795287, Length=185, Percent_Identity=33.5135135135135, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=460, Percent_Identity=37.6086956521739, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=25.4582484725051, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=206, Percent_Identity=31.5533980582524, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=237, Percent_Identity=29.535864978903, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=213, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=251, Percent_Identity=28.2868525896414, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=251, Percent_Identity=28.2868525896414, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=201, Percent_Identity=30.3482587064677, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=37.6623376623377, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=31.0112359550562, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=30, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=39.4273127753304, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24652985, Length=417, Percent_Identity=26.1390887290168, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922090, Length=417, Percent_Identity=26.1390887290168, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24652981, Length=417, Percent_Identity=26.1390887290168, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24652983, Length=417, Percent_Identity=26.1390887290168, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24644968, Length=422, Percent_Identity=25.8293838862559, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648435, Length=485, Percent_Identity=24.7422680412371, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648437, Length=485, Percent_Identity=24.7422680412371, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550774, Length=485, Percent_Identity=24.5360824742268, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.4196642685851, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.4196642685851, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=25.9765625, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=226, Percent_Identity=28.7610619469027, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SULTO (Q971U6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377215.1
- ProteinModelPortal:   Q971U6
- SMR:   Q971U6
- GeneID:   1459282
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1283
- NMPDR:   fig|273063.1.peg.1383
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   STOK273063:ST1283-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52452; Mature: 52321

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 73-73 ACT_SITE 148-148 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSNMIMKLVEDLLNKNLDIDEYVERTYERIEKIEKKIKAFITIRDKEEVKREVREKLKN
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCC
PKGKLSGILIAIKDNISTKGIRTTCASRMLEDYVPPYDATVIEKLKQEGAVILGKTNMDE
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCH
FAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGIVDIALGSDTGGSIRAPAAYN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCEECCHHHH
AVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQ
HEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
VPEKVELKDVKIAVLKDIVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLGYAEYALPAYY
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEEE
IIAMSEASSNLARFDGVRYGYSKYSEGNWRETFAKNRGEGFGIEVKRRILLGSFILSAGY
EEEECCCCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
YEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPKIGEVVEDPIKMYAMDLNTVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIMAISAKIEKVLNLYDLTAPIS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GSNMIMKLVEDLLNKNLDIDEYVERTYERIEKIEKKIKAFITIRDKEEVKREVREKLKN
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCC
PKGKLSGILIAIKDNISTKGIRTTCASRMLEDYVPPYDATVIEKLKQEGAVILGKTNMDE
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCH
FAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGIVDIALGSDTGGSIRAPAAYN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCEECCHHHH
AVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDATTIHFEPQ
HEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
VPEKVELKDVKIAVLKDIVEASDKPVVSIFNSTLDKLSSEGAIIKEVNLGYAEYALPAYY
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEEE
IIAMSEASSNLARFDGVRYGYSKYSEGNWRETFAKNRGEGFGIEVKRRILLGSFILSAGY
EEEECCCCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
YEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPILPPKIGEVVEDPIKMYAMDLNTVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIMAISAKIEKVLNLYDLTAPIS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479