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The map label for this gene is tenA [H]

Identifier: 15921253

GI number: 15921253

Start: 988305

End: 988955

Strand: Direct

Name: tenA [H]

Synonym: ST1007

Alternate gene names: 15921253

Gene position: 988305-988955 (Clockwise)

Preceding gene: 15921252

Following gene: 15921254

Centisome position: 36.68

GC content: 34.72

Gene sequence:

>651_bases
ATGCTATCAGACGAACTTTGGGGCTCGATTTCAGATATTTATTCTTCGATACTATCACATCCATTCATTAAGGGGTTAAC
TGATGGGACATTAGAGGAAGAGAAATTTAAATATTATATCCTGCAAGACTTTTTGTATTTAAGAGAGTTTAGTAGGGCAT
TAGCTATACTATCTGCTAAGGCAGAAAAGCAAGATGATTCAGTCCTTTTCGCTACTCATGTTAGTGATGTATTAAGAGTA
GAAAGGAGCCTTCATGAGTATTTTATCTCTTTATGGAATGTTGATGTTAGTAGGGTTAAGCCATCTCCCACGAATCTTCT
TTATACATCATACTTACTTTCAGTAGTTTATTCGAGACCATTTTATGAAGGTGTTAGTGCTGTTCTTCCATGTTATTGGA
TATATATGGAAGTTGGGAAGGAATTATTAAAGAAGGGTTCTCCAAATCCTTTGTATAAACGTTGGATAGAGACTTACGGC
GGTGAGGAATATGAGAAGGGAGTAAGAGCTGTAATAAATCTAGTTAATTCGTTTAATTTAACTAAGGAAGAAAAAGAGGC
TGTTAAGAGACATTTTAGGATTACGTCAATGTTCGAGTATATGTTCTGGGATATGGCTTATAGGTTAGAAAAGTTCCCAT
TTGATTTTTAA

Upstream 100 bases:

>100_bases
TCTAATAGTTGCCTTATGGACTAAGAATTATAAGTTTAGGACTGCTGCTGTAAGAATAGCAGAATGATTTATTTTTTATC
TCCTTCTACTATTTTATTTT

Downstream 100 bases:

>100_bases
CTTTCTTTTCACAAGTTAGTTTATGAGAACTCTAAACGAACTCGAAGCTAGGCTAACGCTTATTTACGGTAATTCTTTCG
TAAATGTGAAAAGACTAAAC

Product: transcriptional activator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLSDELWGSISDIYSSILSHPFIKGLTDGTLEEEKFKYYILQDFLYLREFSRALAILSAKAEKQDDSVLFATHVSDVLRV
ERSLHEYFISLWNVDVSRVKPSPTNLLYTSYLLSVVYSRPFYEGVSAVLPCYWIYMEVGKELLKKGSPNPLYKRWIETYG
GEEYEKGVRAVINLVNSFNLTKEEKEAVKRHFRITSMFEYMFWDMAYRLEKFPFDF

Sequences:

>Translated_216_residues
MLSDELWGSISDIYSSILSHPFIKGLTDGTLEEEKFKYYILQDFLYLREFSRALAILSAKAEKQDDSVLFATHVSDVLRV
ERSLHEYFISLWNVDVSRVKPSPTNLLYTSYLLSVVYSRPFYEGVSAVLPCYWIYMEVGKELLKKGSPNPLYKRWIETYG
GEEYEKGVRAVINLVNSFNLTKEEKEAVKRHFRITSMFEYMFWDMAYRLEKFPFDF
>Mature_216_residues
MLSDELWGSISDIYSSILSHPFIKGLTDGTLEEEKFKYYILQDFLYLREFSRALAILSAKAEKQDDSVLFATHVSDVLRV
ERSLHEYFISLWNVDVSRVKPSPTNLLYTSYLLSVVYSRPFYEGVSAVLPCYWIYMEVGKELLKKGSPNPLYKRWIETYG
GEEYEKGVRAVINLVNSFNLTKEEKEAVKRHFRITSMFEYMFWDMAYRLEKFPFDF

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=209, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25470; Mature: 25470

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDELWGSISDIYSSILSHPFIKGLTDGTLEEEKFKYYILQDFLYLREFSRALAILSAK
CCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKQDDSVLFATHVSDVLRVERSLHEYFISLWNVDVSRVKPSPTNLLYTSYLLSVVYSRP
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCC
FYEGVSAVLPCYWIYMEVGKELLKKGSPNPLYKRWIETYGGEEYEKGVRAVINLVNSFNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TKEEKEAVKRHFRITSMFEYMFWDMAYRLEKFPFDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSDELWGSISDIYSSILSHPFIKGLTDGTLEEEKFKYYILQDFLYLREFSRALAILSAK
CCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKQDDSVLFATHVSDVLRVERSLHEYFISLWNVDVSRVKPSPTNLLYTSYLLSVVYSRP
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCC
FYEGVSAVLPCYWIYMEVGKELLKKGSPNPLYKRWIETYGGEEYEKGVRAVINLVNSFNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TKEEKEAVKRHFRITSMFEYMFWDMAYRLEKFPFDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]