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The map label for this gene is yoaE [H]

Identifier: 15920859

GI number: 15920859

Start: 623442

End: 625193

Strand: Direct

Name: yoaE [H]

Synonym: ST0639

Alternate gene names: 15920859

Gene position: 623442-625193 (Clockwise)

Preceding gene: 15920858

Following gene: 15920860

Centisome position: 23.14

GC content: 33.73

Gene sequence:

>1752_bases
ATGATTGCATGTACAAGGGACTGTTATGATACTTGTATATTTGATTCTAATTATAAACCTTTGAACATTTTCCCTATAAA
CGGATTTACTTGTTCTAGAGGAATTGCAGATTTAAAAAGAAATGAGATTAACAGAGTGGACTCCGCTTACATAGAGGGTA
AGCCTGTTTCTATAGATGAAGCCGTAAAGTATATAGTAAAAAAGATAAAAGAAACTAAAAGAGAAGAAATTCTTCATATT
GACTATGACGGCAATCAAGGACTATTAACATGGTACTATCCTGCAAGATTTTGGAATGCAATTGGTTCATCATCTACTGA
CTATTCAATTTGCTCTGCTGAAGGGCACGAAGCTATTAAGATTCACTACGGTACTTCTTTTGGTGCGTTACCAGAAGATT
TTGTTAGATATAACGCTGTAGTCTTTTGGGGATCAGAAGCCGTTTTCTCTTTTATTCATGGATGGAGAATTCTATCTAAT
AAATACAAGATAACAATTGATGTAAGAATGAGTGAAACCGCAAAAAGAAGTGAAAAAGCCTATATCATAAACCCAGCTTC
AGATGCGTTTTTAGCAATTGGTGTTATTAAAACGTTATTTTATGAAGGTCTTTATGATCCTTCAATGATTGATAACGTCG
AAATTCTAAAGAATTATGTAAATTCTTATGATTTCTCGTTGATAGAAGAGACTACTGGTTTATCTCAGGAGGAAATTGAA
GAGTTAGCTGAACTTTATTACTATTATAAACCCTTAACAATAATTGGTTTCGCTTTAGGAAGAACATATAATGGCGGTGA
TGCTATCTCATTAATTTCTTTGATTCCCGCTTTATTGGGCATGAAAAGAGGATTCTTTTACTCAAACAGTCAAGGATGGG
GAATAGACTTCTCCTATCTTCGCGGGTTGCATATAGCAAAACCAAGCAGAATTATAGGTATGGCTGAAGTAGGGAAAGAA
GTTGAAAAAGGAAATGTAAAGTTTCTATTCACTTGGAACTCTAATCCTATTCATTCTTTGCCAGGAAGTGATAAAATAAA
AGAAGCTGTCGAAGAAGGAAAGTTGTTTTTAGTTTCTCATGATCCCTTTTGGAATGAGACCACAAAAATAGCAAATGTTG
TTATTCCAGCACCAACTTTTTTAGAAAAGTATGATGTAGTATATAGTTATTGGCACAGCTATCTAGTCTATAACGAACCT
ATTAGACCACAAAGAGGGATTACTGAAGTTGAATTGATGAAAAAGCTTGCAAAAGAATATGGGATTATTTCTCATCCTCT
CATTGAGGAAAACGAATGGATTGCTGTAGATAACGCAATAAGAGGTACTGGAGTTTCTTTACAAGAATTAAAAGACAAGA
AGATAGTTAAAATGAATAAGACTATTGAAGTTAACAAAGTTAAAGTTGAGCCTCTACCCAAACTAACTCCTCCTCCTAAG
GGAGTTTACTTGGTCTTCTCTTCACATCCTAACCATACAAATTCTCAATTTAAGGAAATATACTCTCAGATGCCTATAGC
ATATAATAATCAACTTGATGGTATAGGTTATCTAGAAAATTCCAATGGGAAAGTTAAAGTACTTCTGAAAAAGGATGAGA
GCGTACCCAACAATGTAGTTTTCATGTTTAAGAGTTCCTTAATTGGATTAGATGACAAGCCAATCAACTCGCTTATTGGT
AGTGAAAAAGGAAAATATGGAGGAACTCCATTACTTAACGGGCATACTGTTAATATTAGATTAATAAAATAA

Upstream 100 bases:

>100_bases
AATGGATTTTAGAGCATTCAGCTTAAAGGAATTTCTTCACCATTCAGTTACGGTCGCACACATCACTAAGTTTTAATATA
TACTAGACTATAAAAGAATA

Downstream 100 bases:

>100_bases
ATGTTATACTATTGAATAACTATTATATTGTAATACCACAAATAAAAGCATAATCTTAATTAATTACTGTAGTCAGACAT
AATCTTGACCAGAAATGGTA

Product: hypothetical protein

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MIACTRDCYDTCIFDSNYKPLNIFPINGFTCSRGIADLKRNEINRVDSAYIEGKPVSIDEAVKYIVKKIKETKREEILHI
DYDGNQGLLTWYYPARFWNAIGSSSTDYSICSAEGHEAIKIHYGTSFGALPEDFVRYNAVVFWGSEAVFSFIHGWRILSN
KYKITIDVRMSETAKRSEKAYIINPASDAFLAIGVIKTLFYEGLYDPSMIDNVEILKNYVNSYDFSLIEETTGLSQEEIE
ELAELYYYYKPLTIIGFALGRTYNGGDAISLISLIPALLGMKRGFFYSNSQGWGIDFSYLRGLHIAKPSRIIGMAEVGKE
VEKGNVKFLFTWNSNPIHSLPGSDKIKEAVEEGKLFLVSHDPFWNETTKIANVVIPAPTFLEKYDVVYSYWHSYLVYNEP
IRPQRGITEVELMKKLAKEYGIISHPLIEENEWIAVDNAIRGTGVSLQELKDKKIVKMNKTIEVNKVKVEPLPKLTPPPK
GVYLVFSSHPNHTNSQFKEIYSQMPIAYNNQLDGIGYLENSNGKVKVLLKKDESVPNNVVFMFKSSLIGLDDKPINSLIG
SEKGKYGGTPLLNGHTVNIRLIK

Sequences:

>Translated_583_residues
MIACTRDCYDTCIFDSNYKPLNIFPINGFTCSRGIADLKRNEINRVDSAYIEGKPVSIDEAVKYIVKKIKETKREEILHI
DYDGNQGLLTWYYPARFWNAIGSSSTDYSICSAEGHEAIKIHYGTSFGALPEDFVRYNAVVFWGSEAVFSFIHGWRILSN
KYKITIDVRMSETAKRSEKAYIINPASDAFLAIGVIKTLFYEGLYDPSMIDNVEILKNYVNSYDFSLIEETTGLSQEEIE
ELAELYYYYKPLTIIGFALGRTYNGGDAISLISLIPALLGMKRGFFYSNSQGWGIDFSYLRGLHIAKPSRIIGMAEVGKE
VEKGNVKFLFTWNSNPIHSLPGSDKIKEAVEEGKLFLVSHDPFWNETTKIANVVIPAPTFLEKYDVVYSYWHSYLVYNEP
IRPQRGITEVELMKKLAKEYGIISHPLIEENEWIAVDNAIRGTGVSLQELKDKKIVKMNKTIEVNKVKVEPLPKLTPPPK
GVYLVFSSHPNHTNSQFKEIYSQMPIAYNNQLDGIGYLENSNGKVKVLLKKDESVPNNVVFMFKSSLIGLDDKPINSLIG
SEKGKYGGTPLLNGHTVNIRLIK
>Mature_583_residues
MIACTRDCYDTCIFDSNYKPLNIFPINGFTCSRGIADLKRNEINRVDSAYIEGKPVSIDEAVKYIVKKIKETKREEILHI
DYDGNQGLLTWYYPARFWNAIGSSSTDYSICSAEGHEAIKIHYGTSFGALPEDFVRYNAVVFWGSEAVFSFIHGWRILSN
KYKITIDVRMSETAKRSEKAYIINPASDAFLAIGVIKTLFYEGLYDPSMIDNVEILKNYVNSYDFSLIEETTGLSQEEIE
ELAELYYYYKPLTIIGFALGRTYNGGDAISLISLIPALLGMKRGFFYSNSQGWGIDFSYLRGLHIAKPSRIIGMAEVGKE
VEKGNVKFLFTWNSNPIHSLPGSDKIKEAVEEGKLFLVSHDPFWNETTKIANVVIPAPTFLEKYDVVYSYWHSYLVYNEP
IRPQRGITEVELMKKLAKEYGIISHPLIEENEWIAVDNAIRGTGVSLQELKDKKIVKMNKTIEVNKVKVEPLPKLTPPPK
GVYLVFSSHPNHTNSQFKEIYSQMPIAYNNQLDGIGYLENSNGKVKVLLKKDESVPNNVVFMFKSSLIGLDDKPINSLIG
SEKGKYGGTPLLNGHTVNIRLIK

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 65990; Mature: 65990

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIACTRDCYDTCIFDSNYKPLNIFPINGFTCSRGIADLKRNEINRVDSAYIEGKPVSIDE
CCCCCCCHHHHHEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHH
AVKYIVKKIKETKREEILHIDYDGNQGLLTWYYPARFWNAIGSSSTDYSICSAEGHEAIK
HHHHHHHHHHHHHHHCEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCEEEECCCCCEEE
IHYGTSFGALPEDFVRYNAVVFWGSEAVFSFIHGWRILSNKYKITIDVRMSETAKRSEKA
EEECCCCCCCCHHHHHEEEEEEECCHHHHHHHHHHHEECCCEEEEEEEEECCHHHCCCCE
YIINPASDAFLAIGVIKTLFYEGLYDPSMIDNVEILKNYVNSYDFSLIEETTGLSQEEIE
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCEEHHHHCCCCHHHHH
ELAELYYYYKPLTIIGFALGRTYNGGDAISLISLIPALLGMKRGFFYSNSQGWGIDFSYL
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHH
RGLHIAKPSRIIGMAEVGKEVEKGNVKFLFTWNSNPIHSLPGSDKIKEAVEEGKLFLVSH
CCEEECCCCCEEEHHHHCCHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEC
DPFWNETTKIANVVIPAPTFLEKYDVVYSYWHSYLVYNEPIRPQRGITEVELMKKLAKEY
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHC
GIISHPLIEENEWIAVDNAIRGTGVSLQELKDKKIVKMNKTIEVNKVKVEPLPKLTPPPK
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCEEEECCEEEEEEEEECCCCCCCCCCC
GVYLVFSSHPNHTNSQFKEIYSQMPIAYNNQLDGIGYLENSNGKVKVLLKKDESVPNNVV
CEEEEEECCCCCCHHHHHHHHHHCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEE
FMFKSSLIGLDDKPINSLIGSEKGKYGGTPLLNGHTVNIRLIK
EEEECCCCCCCCCCHHHHHCCCCCCCCCCCEECCEEEEEEEEC
>Mature Secondary Structure
MIACTRDCYDTCIFDSNYKPLNIFPINGFTCSRGIADLKRNEINRVDSAYIEGKPVSIDE
CCCCCCCHHHHHEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHH
AVKYIVKKIKETKREEILHIDYDGNQGLLTWYYPARFWNAIGSSSTDYSICSAEGHEAIK
HHHHHHHHHHHHHHHCEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCEEEECCCCCEEE
IHYGTSFGALPEDFVRYNAVVFWGSEAVFSFIHGWRILSNKYKITIDVRMSETAKRSEKA
EEECCCCCCCCHHHHHEEEEEEECCHHHHHHHHHHHEECCCEEEEEEEEECCHHHCCCCE
YIINPASDAFLAIGVIKTLFYEGLYDPSMIDNVEILKNYVNSYDFSLIEETTGLSQEEIE
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCEEHHHHCCCCHHHHH
ELAELYYYYKPLTIIGFALGRTYNGGDAISLISLIPALLGMKRGFFYSNSQGWGIDFSYL
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHH
RGLHIAKPSRIIGMAEVGKEVEKGNVKFLFTWNSNPIHSLPGSDKIKEAVEEGKLFLVSH
CCEEECCCCCEEEHHHHCCHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEC
DPFWNETTKIANVVIPAPTFLEKYDVVYSYWHSYLVYNEPIRPQRGITEVELMKKLAKEY
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHC
GIISHPLIEENEWIAVDNAIRGTGVSLQELKDKKIVKMNKTIEVNKVKVEPLPKLTPPPK
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCEEEECCEEEEEEEEECCCCCCCCCCC
GVYLVFSSHPNHTNSQFKEIYSQMPIAYNNQLDGIGYLENSNGKVKVLLKKDESVPNNVV
CEEEEEECCCCCCHHHHHHHHHHCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEE
FMFKSSLIGLDDKPINSLIGSEKGKYGGTPLLNGHTVNIRLIK
EEEECCCCCCCCCCHHHHHCCCCCCCCCCCEECCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]