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The map label for this gene is aksA

Identifier: 15920756

GI number: 15920756

Start: 533701

End: 534861

Strand: Reverse

Name: aksA

Synonym: ST0538

Alternate gene names: 15920756

Gene position: 534861-533701 (Counterclockwise)

Preceding gene: 15920757

Following gene: 15920755

Centisome position: 19.85

GC content: 34.97

Gene sequence:

>1161_bases
TTGGGATGTCAATTCTCGGTTAATTCAAAAAAAGTTAGAATATTTGATACAACATTAAGAGATGGCGAGCAAGCTCCGGG
TATAGATTTAACAGTCGAACAAAAAGTTAAGATAGCGAGAAAATTGGCAGAACTAGGAGTTGATGTAATAGAAGCTGGTT
TTCCTGCAGCTTCTGAGGGAGAATTTATAGCCACTAAGAAAATCCTAGAAGAAGTTGGAGATCAGGTTGAGGTTATAGGA
TTATCAAGGGCGAATAAGCAAGATATAGATAAGACCATCGATACTGGCATATCAAGCATCCATGTATTCATTGCTACTTC
TGATATACATCTGAAATATAAACTAAAAATGACAAGGGAACAAGTACTAGATAAAATTTATGAATCAGTTAGATATGCTA
AAGATCATGGCTTAATAGTTGAATATTCCCCAGAAGATGCTACAAGAACTGATAAGGATTTCTTATTAAAAGCTGTAAGT
ACTGCTATTGAGGCTGGGGCAGATAGAATAAATATACCAGATACTGTTGGAGTTATGCATCCCTTCAAGTTTCATGATTT
AATCAAAGATGTTGTTTCTGTAACTAAAGATAAAATCGTAAGTGTACACTGCCATAATGATTTTGGTTTAGCAACAGCTA
ACTCAATTGCTGGAGTAATGGCTGGGGCAAGACAAGTTCATGTAACTGTGAATGGAATTGGAGAGAGAGCTGGAAATGCA
TCATTAGAAGAAGTAGTGATGGCACTGAAAAAACTTTTGGGATATGAGGTTAATATCAAAACATATAAGTTATACGAAAC
AAGCAGACTTGTATCAGAGTTAACTGGTGTTCCTGTTCCCTATTTTAAAGCGATAGTAGGAGAAAATGCATTTGGTCATG
AAGCTGGCATCCATGTTCACGGAGTTATTGAGAACCCATTGACATATGAGCCAATATCTCCAGAGGAGGTAGGTAATTTC
AGAAGATTAGCACTAGGAAAACATAGTGGTATACATGGTTTGAAAAAACTTCTTGAGGAACAAGGAATTTACTTAAATGA
TCAAGAGTTAAGAGAAGTATTAAATGAAATAAAGAAACTTGCTGAAAATGGTGAAAAGGTAAATGTTGATGTTGCTAAAG
AGATAGCTATTAAAGTAAGTAGCAAAAAGATTAAAGTATGA

Upstream 100 bases:

>100_bases
TACCAGCTAACTGGGTTAAAAGAAACAAACTAAAAATAGCTAAACAGATAATCGAACAAGTTTTAAGCAAAGATTTATAA
TTTTTTAAGTAACAGATAGT

Downstream 100 bases:

>100_bases
TAAAGTTTTTCTATCATTCTTCTTTTCTTCTAGATAATAAAATACTTTTAGATCCTCATGATGGAGGAAGTATAGGCTTA
CCCAGACCTGAAACTAAAGC

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MGCQFSVNSKKVRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEGEFIATKKILEEVGDQVEVIG
LSRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTREQVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVS
TAIEAGADRINIPDTVGVMHPFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNA
SLEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVHGVIENPLTYEPISPEEVGNF
RRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKLAENGEKVNVDVAKEIAIKVSSKKIKV

Sequences:

>Translated_386_residues
MGCQFSVNSKKVRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEGEFIATKKILEEVGDQVEVIG
LSRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTREQVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVS
TAIEAGADRINIPDTVGVMHPFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNA
SLEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVHGVIENPLTYEPISPEEVGNF
RRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKLAENGEKVNVDVAKEIAIKVSSKKIKV
>Mature_385_residues
GCQFSVNSKKVRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEGEFIATKKILEEVGDQVEVIGL
SRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTREQVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVST
AIEAGADRINIPDTVGVMHPFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNAS
LEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVHGVIENPLTYEPISPEEVGNFR
RLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKLAENGEKVNVDVAKEIAIKVSSKKIKV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=295, Percent_Identity=24.7457627118644, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI109150427, Length=301, Percent_Identity=22.5913621262458, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI109150422, Length=301, Percent_Identity=22.5913621262458, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786261, Length=365, Percent_Identity=43.013698630137, Blast_Score=268, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6320071, Length=358, Percent_Identity=31.5642458100559, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320019, Length=358, Percent_Identity=31.2849162011173, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324682, Length=347, Percent_Identity=29.6829971181556, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=348, Percent_Identity=28.735632183908, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582381, Length=289, Percent_Identity=25.2595155709343, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_SULTO (Q974X3)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376425.1
- ProteinModelPortal:   Q974X3
- SMR:   Q974X3
- GeneID:   1458484
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0538
- NMPDR:   fig|273063.1.peg.593
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- ProtClustDB:   PRK11858
- BioCyc:   STOK273063:ST0538-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 42433; Mature: 42302

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCQFSVNSKKVRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEG
CCCEECCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCC
EFIATKKILEEVGDQVEVIGLSRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTRE
CCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEEEHHHHH
QVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVSTAIEAGADRINIPDTVGVMH
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNA
CHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCC
SLEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVH
CHHHHHHHHHHHHCCEECEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEE
GVIENPLTYEPISPEEVGNFRRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKL
EEECCCCCCCCCCHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHH
AENGEKVNVDVAKEIAIKVSSKKIKV
HHCCCEEEEEEHEEEEEEECCCEECC
>Mature Secondary Structure 
GCQFSVNSKKVRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEG
CCEECCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCC
EFIATKKILEEVGDQVEVIGLSRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTRE
CCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCEEEEEEEHHHHH
QVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVSTAIEAGADRINIPDTVGVMH
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNA
CHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCC
SLEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVH
CHHHHHHHHHHHHCCEECEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEE
GVIENPLTYEPISPEEVGNFRRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKL
EEECCCCCCCCCCHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHH
AENGEKVNVDVAKEIAIKVSSKKIKV
HHCCCEEEEEEHEEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479