Click here to switch to the map view.

The map label for this gene is speB [C]

Identifier: 15920554

GI number: 15920554

Start: 373125

End: 374012

Strand: Reverse

Name: speB [C]

Synonym: ST0352

Alternate gene names: 15920554

Gene position: 374012-373125 (Counterclockwise)

Preceding gene: 15920561

Following gene: 15920552

Centisome position: 13.88

GC content: 34.35

Gene sequence:

>888_bases
ATGTCAGATAGCAGGTTACTATACTTAAATGAAAATAGTAGATTATTTGCCGGATTTAATAAACCAACATCTCCATTTGT
TATAATTGGTCTACCATTAGATATAACAAGTAGTTTCAGACCAGGTTCCAGATTTGCACCATCTACCATAAGAGAGTACG
CACAATTTATTGAGTTTTATTCTATAAGAACTGGAATAGATATGGGAGAAGTAGGGTTTAATGATGTAGGAGATGTAGTA
ATGCATCCCTCTGATGTAGAAGAAAATATAAGAAGAATATCAGACGTAACTAGTTATTTTGCCGAAAAAGGAAAAATAAT
AATAGGTATAGGTGGAGAACATTCTGTAACTGTAGGAACTGTAAGAGGAATTAAACCAGACTGTGTACTTAGTATTGACG
CACATCTAGACCTTAGAGATGAATACATGGGTTATAAATACGACCACGCTTGTGTTATGAGACGAATATCAGAACAGGGA
GTTAAAATAATGGAAATAGCTACAAGAGCTGTCTCTAAAGAAGAATTAGATTACGCAAATAAAAACGGAATAGCTTATCT
TACACCACATCAAATAAGATTATTAGGAGTAAGAGAGACTGCAAAGAAAATTGTTAACAATTTCAGAGATTGTGAAAAAA
TCTATGTAACATATGATATGGATGGAATAGATCCAGCATATGCTCCAGGTGTAGCTACTCCAGAGCCTGAAGGATTAGAC
CCAACTACAGTACTAGATATTATATCGCTTATAATAGATAAAAGAGTTGTAGGCTTTGATGTAGTAGAAGTTTCACCACC
TCATGATCCATCTGGAATAACTTCAGTATTAGGAGCCAGAATAATCTTAGAAACATCTGCCCAGATTTATAAGGCTAGAT
CGCTTTAA

Upstream 100 bases:

>100_bases
TCATATGAAGGCCAAAATATTCCTCTTCTTTTCGCCAATTCAATTAACTTCTCACTCATACGAGATAAGCATATAAACAA
GATTAAAAATTTCTACTCTC

Downstream 100 bases:

>100_bases
CAACCTTTACTGTAGGTTTTCTTACCATATAGATTATTTTATAGCCGCAATAAGGACATCTCACACCTGGAAGAGCTCTT
AATTGTTCATCATCAAATTC

Product: agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSDSRLLYLNENSRLFAGFNKPTSPFVIIGLPLDITSSFRPGSRFAPSTIREYAQFIEFYSIRTGIDMGEVGFNDVGDVV
MHPSDVEENIRRISDVTSYFAEKGKIIIGIGGEHSVTVGTVRGIKPDCVLSIDAHLDLRDEYMGYKYDHACVMRRISEQG
VKIMEIATRAVSKEELDYANKNGIAYLTPHQIRLLGVRETAKKIVNNFRDCEKIYVTYDMDGIDPAYAPGVATPEPEGLD
PTTVLDIISLIIDKRVVGFDVVEVSPPHDPSGITSVLGARIILETSAQIYKARSL

Sequences:

>Translated_295_residues
MSDSRLLYLNENSRLFAGFNKPTSPFVIIGLPLDITSSFRPGSRFAPSTIREYAQFIEFYSIRTGIDMGEVGFNDVGDVV
MHPSDVEENIRRISDVTSYFAEKGKIIIGIGGEHSVTVGTVRGIKPDCVLSIDAHLDLRDEYMGYKYDHACVMRRISEQG
VKIMEIATRAVSKEELDYANKNGIAYLTPHQIRLLGVRETAKKIVNNFRDCEKIYVTYDMDGIDPAYAPGVATPEPEGLD
PTTVLDIISLIIDKRVVGFDVVEVSPPHDPSGITSVLGARIILETSAQIYKARSL
>Mature_294_residues
SDSRLLYLNENSRLFAGFNKPTSPFVIIGLPLDITSSFRPGSRFAPSTIREYAQFIEFYSIRTGIDMGEVGFNDVGDVVM
HPSDVEENIRRISDVTSYFAEKGKIIIGIGGEHSVTVGTVRGIKPDCVLSIDAHLDLRDEYMGYKYDHACVMRRISEQGV
KIMEIATRAVSKEELDYANKNGIAYLTPHQIRLLGVRETAKKIVNNFRDCEKIYVTYDMDGIDPAYAPGVATPEPEGLDP
TTVLDIISLIIDKRVVGFDVVEVSPPHDPSGITSVLGARIILETSAQIYKARSL

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=277, Percent_Identity=31.4079422382672, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1789306, Length=258, Percent_Identity=28.6821705426357, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32712; Mature: 32581

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSRLLYLNENSRLFAGFNKPTSPFVIIGLPLDITSSFRPGSRFAPSTIREYAQFIEFY
CCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIRTGIDMGEVGFNDVGDVVMHPSDVEENIRRISDVTSYFAEKGKIIIGIGGEHSVTVGT
HHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEE
VRGIKPDCVLSIDAHLDLRDEYMGYKYDHACVMRRISEQGVKIMEIATRAVSKEELDYAN
ECCCCCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KNGIAYLTPHQIRLLGVRETAKKIVNNFRDCEKIYVTYDMDGIDPAYAPGVATPEPEGLD
CCCEEEECCCEEEEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
PTTVLDIISLIIDKRVVGFDVVEVSPPHDPSGITSVLGARIILETSAQIYKARSL
HHHHHHHHHHHHCCHHCCEEEEEECCCCCCHHHHHHHHHHEEEECCHHHHHHCCC
>Mature Secondary Structure 
SDSRLLYLNENSRLFAGFNKPTSPFVIIGLPLDITSSFRPGSRFAPSTIREYAQFIEFY
CCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIRTGIDMGEVGFNDVGDVVMHPSDVEENIRRISDVTSYFAEKGKIIIGIGGEHSVTVGT
HHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEE
VRGIKPDCVLSIDAHLDLRDEYMGYKYDHACVMRRISEQGVKIMEIATRAVSKEELDYAN
ECCCCCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KNGIAYLTPHQIRLLGVRETAKKIVNNFRDCEKIYVTYDMDGIDPAYAPGVATPEPEGLD
CCCEEEECCCEEEEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
PTTVLDIISLIIDKRVVGFDVVEVSPPHDPSGITSVLGARIILETSAQIYKARSL
HHHHHHHHHHHHCCHHCCEEEEEECCCCCCHHHHHHHHHHEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]