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The map label for this gene is tgtA

Identifier: 15920526

GI number: 15920526

Start: 347189

End: 348664

Strand: Direct

Name: tgtA

Synonym: ST0327

Alternate gene names: 15920526

Gene position: 347189-348664 (Clockwise)

Preceding gene: 15920525

Following gene: 15920530

Centisome position: 12.88

GC content: 29.47

Gene sequence:

>1476_bases
ATGGTAGGAGAATTTGAAATAAAAGATGAAGATCTAGCTGGTAGAATAGGAATCATAGAGACAAAAAGTGGAAAACTAGA
AACACCGGTATTCTTTCCAGTAATTAATCCTTTTAAATCTGAAATCTCGATAAAAGATATAGAAAATTTAGGGTTTAAGA
ATTTAATCACAAATGCATATTTAATAAAAAAGATAACTAATACTAAAATTCAAGATATTCATAGCTTTTTACAATTTAAT
GGTGTAATAATGACTGATTCTGGAGCTTATCAGATACTTCAATACGGAAACATAGAAGTTACAAATAGAGAGATAGTTGA
GTACGAGAGGGATATAAATACAGATATAGCAGTCTATTTAGATCTTCCTACTGGTGATACAAATAGCAGAGATGAAGCTA
TTAACAGTGTAAAAATCACGTTAGAGAGGGCAAAAGAAATAGAGGATATAGTCAAAAATGATTCAGAAAGGATTTGGGTA
CACCCCATTCAAGGTGGAAGATTTCTAGACCTTGTTGAATATTCGGCGATAGAGGCTGATAAAAACGAGGCATTTAAAAT
GCTAGCATTAGGAAGTCCTACAGTAGTAATGGAAAAGTACGACTATTCCTTATTGATCGATATGGTATTTATAGCAAAAA
GTAATGTAAGTAGAGGCAAACCTTTTCATCTTTTTGGTGGCGGTCTTCCTCATATAATGCCTCTTGTTATAGCACTAGGT
GTAGATTCTTTTGATTCTGCATCATATATACTTTATGCTAGGGATAATAGGTACATAACGAGAAGTAGAGTATATAGACT
AGAAGAGTTAGAATATTTTCCATGTTCATGTCCAATCTGCTTAAAATACACACCCAAAGAGCTGCTAGAGTTACCAAAAG
AAGAAAGAACAAAACTTTTGGCTCTTCATAATCTTTATGTAATTAAGGAAGAACTTAATGCTATAAAACAAGCTATTAGA
GAAGGAAGATTATTTGAATATATTCAAGAAAAAGCATACTCTCACCCAGCAGTGTATTCTGCTTTTAAAAAAATATTAAA
ATATAAAGATTACTTAGAAAAATATGATCCAAGAGTAAAAGGAAATATTAGAGGAATTTTCTTGTTCGATCTTAAGTCTT
TAAATAGACCAGAAATTGTTAGGCATTATAATTTCTTAGATAGGATTAATAAGAATAACGATAAAGCTATTATAATTTGT
GAAAAAAGAGAAAATATATTAAAAAAAGTGAAAAATAATGAGAATGTAGACATATATCTTTTTAATCCTTTTTATGGTTT
AATACCGATAAATCTAATAGAAGTCTATCCATACTTTCAAACTGAATATCCAGAGGAAATTGATGAAGATGTTTTACGTT
ATATTGAAAGTAAAATAGTTGAATTTGTAAGATCTAAAGGATATACAAAAATTGATTTCATAGGTTGTGAAAAATATTTA
TCACATATAAACTCTATTGGAGCCTTCTTTAGTTAA

Upstream 100 bases:

>100_bases
AAAGTTTCTGAAAATGGAGTGGAAGGAAGCGGACCCTTGGCACAAAGAATTTACGATTTATATAACGATTATATAAATAA
GAAAAAGAAAGGATCTTAAA

Downstream 100 bases:

>100_bases
TTCTTTTTGAACTAACTCTAATTGCCTTTGCAATTCTTCTTCAATCTTTCTTGCATCTTCGTATAGTTCTGACACATCAA
CCTTTAATCCATATAATTTA

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MVGEFEIKDEDLAGRIGIIETKSGKLETPVFFPVINPFKSEISIKDIENLGFKNLITNAYLIKKITNTKIQDIHSFLQFN
GVIMTDSGAYQILQYGNIEVTNREIVEYERDINTDIAVYLDLPTGDTNSRDEAINSVKITLERAKEIEDIVKNDSERIWV
HPIQGGRFLDLVEYSAIEADKNEAFKMLALGSPTVVMEKYDYSLLIDMVFIAKSNVSRGKPFHLFGGGLPHIMPLVIALG
VDSFDSASYILYARDNRYITRSRVYRLEELEYFPCSCPICLKYTPKELLELPKEERTKLLALHNLYVIKEELNAIKQAIR
EGRLFEYIQEKAYSHPAVYSAFKKILKYKDYLEKYDPRVKGNIRGIFLFDLKSLNRPEIVRHYNFLDRINKNNDKAIIIC
EKRENILKKVKNNENVDIYLFNPFYGLIPINLIEVYPYFQTEYPEEIDEDVLRYIESKIVEFVRSKGYTKIDFIGCEKYL
SHINSIGAFFS

Sequences:

>Translated_491_residues
MVGEFEIKDEDLAGRIGIIETKSGKLETPVFFPVINPFKSEISIKDIENLGFKNLITNAYLIKKITNTKIQDIHSFLQFN
GVIMTDSGAYQILQYGNIEVTNREIVEYERDINTDIAVYLDLPTGDTNSRDEAINSVKITLERAKEIEDIVKNDSERIWV
HPIQGGRFLDLVEYSAIEADKNEAFKMLALGSPTVVMEKYDYSLLIDMVFIAKSNVSRGKPFHLFGGGLPHIMPLVIALG
VDSFDSASYILYARDNRYITRSRVYRLEELEYFPCSCPICLKYTPKELLELPKEERTKLLALHNLYVIKEELNAIKQAIR
EGRLFEYIQEKAYSHPAVYSAFKKILKYKDYLEKYDPRVKGNIRGIFLFDLKSLNRPEIVRHYNFLDRINKNNDKAIIIC
EKRENILKKVKNNENVDIYLFNPFYGLIPINLIEVYPYFQTEYPEEIDEDVLRYIESKIVEFVRSKGYTKIDFIGCEKYL
SHINSIGAFFS
>Mature_491_residues
MVGEFEIKDEDLAGRIGIIETKSGKLETPVFFPVINPFKSEISIKDIENLGFKNLITNAYLIKKITNTKIQDIHSFLQFN
GVIMTDSGAYQILQYGNIEVTNREIVEYERDINTDIAVYLDLPTGDTNSRDEAINSVKITLERAKEIEDIVKNDSERIWV
HPIQGGRFLDLVEYSAIEADKNEAFKMLALGSPTVVMEKYDYSLLIDMVFIAKSNVSRGKPFHLFGGGLPHIMPLVIALG
VDSFDSASYILYARDNRYITRSRVYRLEELEYFPCSCPICLKYTPKELLELPKEERTKLLALHNLYVIKEELNAIKQAIR
EGRLFEYIQEKAYSHPAVYSAFKKILKYKDYLEKYDPRVKGNIRGIFLFDLKSLNRPEIVRHYNFLDRINKNNDKAIIIC
EKRENILKKVKNNENVDIYLFNPFYGLIPINLIEVYPYFQTEYPEEIDEDVLRYIESKIVEFVRSKGYTKIDFIGCEKYL
SHINSIGAFFS

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family

Homologues:

Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=23.5616438356164, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=24.585635359116, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_SULTO (Q975U5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376195.1
- ProteinModelPortal:   Q975U5
- SMR:   Q975U5
- GeneID:   1458241
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0327
- NMPDR:   fig|273063.1.peg.363
- HOGENOM:   HBG512800
- OMA:   ITNSYIT
- ProtClustDB:   PRK13533
- BioCyc:   STOK273063:ST0327-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 56987; Mature: 56987

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50890 PUA; PS00626 RCC1_2

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGEFEIKDEDLAGRIGIIETKSGKLETPVFFPVINPFKSEISIKDIENLGFKNLITNAY
CCCCEECCCCCCCCCEEEEECCCCCCCCCEEEECCCCHHHCCCHHHHHHCCHHHHHHHHH
LIKKITNTKIQDIHSFLQFNGVIMTDSGAYQILQYGNIEVTNREIVEYERDINTDIAVYL
HHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEECCEEECCHHHHHHHHCCCCCEEEEE
DLPTGDTNSRDEAINSVKITLERAKEIEDIVKNDSERIWVHPIQGGRFLDLVEYSAIEAD
ECCCCCCCCHHHHHHHHEEEHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHHCCC
KNEAFKMLALGSPTVVMEKYDYSLLIDMVFIAKSNVSRGKPFHLFGGGLPHIMPLVIALG
CCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHC
VDSFDSASYILYARDNRYITRSRVYRLEELEYFPCSCPICLKYTPKELLELPKEERTKLL
CCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCHHHHHHEE
ALHNLYVIKEELNAIKQAIREGRLFEYIQEKAYSHPAVYSAFKKILKYKDYLEKYDPRVK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GNIRGIFLFDLKSLNRPEIVRHYNFLDRINKNNDKAIIICEKRENILKKVKNNENVDIYL
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEE
FNPFYGLIPINLIEVYPYFQTEYPEEIDEDVLRYIESKIVEFVRSKGYTKIDFIGCEKYL
ECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
SHINSIGAFFS
HHHHHHHHCCC
>Mature Secondary Structure
MVGEFEIKDEDLAGRIGIIETKSGKLETPVFFPVINPFKSEISIKDIENLGFKNLITNAY
CCCCEECCCCCCCCCEEEEECCCCCCCCCEEEECCCCHHHCCCHHHHHHCCHHHHHHHHH
LIKKITNTKIQDIHSFLQFNGVIMTDSGAYQILQYGNIEVTNREIVEYERDINTDIAVYL
HHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEECCEEECCHHHHHHHHCCCCCEEEEE
DLPTGDTNSRDEAINSVKITLERAKEIEDIVKNDSERIWVHPIQGGRFLDLVEYSAIEAD
ECCCCCCCCHHHHHHHHEEEHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHHCCC
KNEAFKMLALGSPTVVMEKYDYSLLIDMVFIAKSNVSRGKPFHLFGGGLPHIMPLVIALG
CCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHC
VDSFDSASYILYARDNRYITRSRVYRLEELEYFPCSCPICLKYTPKELLELPKEERTKLL
CCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCHHHHHHEE
ALHNLYVIKEELNAIKQAIREGRLFEYIQEKAYSHPAVYSAFKKILKYKDYLEKYDPRVK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GNIRGIFLFDLKSLNRPEIVRHYNFLDRINKNNDKAIIICEKRENILKKVKNNENVDIYL
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEE
FNPFYGLIPINLIEVYPYFQTEYPEEIDEDVLRYIESKIVEFVRSKGYTKIDFIGCEKYL
ECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
SHINSIGAFFS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479