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The map label for this gene is hemC

Identifier: 15920398

GI number: 15920398

Start: 239013

End: 239897

Strand: Direct

Name: hemC

Synonym: ST0217

Alternate gene names: 15920398

Gene position: 239013-239897 (Clockwise)

Preceding gene: 15920397

Following gene: 15920399

Centisome position: 8.87

GC content: 29.49

Gene sequence:

>885_bases
ATGAGAATAAGAATAGCCGCTAGAGGTAGTAAACTAAGCAGAATTCAGGTAATGATGGTTGAAAACTATTTACATAAATT
AGGTATAGAAACTGAATTTATAGAAATTAAAACTAAAGCTGATTTATTCCAGAACGAACCTTTATCTAAACTAGGTAAAG
GAGTATTCGAAAAAGAAGTAAATCAAGCGGTTTTAGATAATAAGGCTGATGTCGCTGTTCATAGTATGAAGGATATCTTG
ACTGAGATTTCAGAAAACTTGGAAATTTATGCTGTATTACCTAGGGATCCTCCATTTGATATTTTAATCTCAAGAAAAAA
CTTATTTAACTTGGAACCAAATTCAATTATAGGAACTAGTAGTATTAGAAGAAAAAATTTCCTAACTTTTTATAGAAATG
ATCTTAACATTAAGGATTTGAGAGGAAATATAGACACTAGATTAAAGAAGTATTTTGAAGGTCAATATGACGGCATTATA
ATAGCTGAGGCTTCAATATATAGGTTAAAAGAGCAGGTTCAGTATTATAGGTTAGATCCTCATTTATTCACTCCAGAAGC
TAACCAGGGTATAATAGTAGTTATAGGAAGAAAAAAAGATGAAACAATAAAGAAAATATTTAATGAAATAAATGATAAAG
ATACATTAGAAATTGCTATTGCTGAAAGAAAAGCATTAAGTATTGTAGGGGGTGGATGCCATTCACCAATTGGAGTTTAT
TTTGAAAAATATGATAAAGATTTCCATGGAATAATTAGTTCTTCATTTGGTAGGAAAAAGATTACAATAGAAGAGTATTT
TCATAACATGACTCCTTATGAAGCAGGGGAATTATTAGGTAAAAGATTCTTGGAGGAAATAAAAAATGAAGGTATTATTC
CTTAG

Upstream 100 bases:

>100_bases
TTCCTCCTAGTCAATTTGAGACAATATTTACATCTAGTTCACACTCTGATGAGATAGTAAATCTTACTATTGAAGCAATT
CATAAGGTGGTTAGCGAAAT

Downstream 100 bases:

>100_bases
GCCCGAAGGATCTAATATTCCTTATAATAAGAATTTTATTGAAATTAGTATTTTAAAACCTGAATGTATAAATTATACTG
TTAATTTACACGAGATAGAC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRIRIAARGSKLSRIQVMMVENYLHKLGIETEFIEIKTKADLFQNEPLSKLGKGVFEKEVNQAVLDNKADVAVHSMKDIL
TEISENLEIYAVLPRDPPFDILISRKNLFNLEPNSIIGTSSIRRKNFLTFYRNDLNIKDLRGNIDTRLKKYFEGQYDGII
IAEASIYRLKEQVQYYRLDPHLFTPEANQGIIVVIGRKKDETIKKIFNEINDKDTLEIAIAERKALSIVGGGCHSPIGVY
FEKYDKDFHGIISSSFGRKKITIEEYFHNMTPYEAGELLGKRFLEEIKNEGIIP

Sequences:

>Translated_294_residues
MRIRIAARGSKLSRIQVMMVENYLHKLGIETEFIEIKTKADLFQNEPLSKLGKGVFEKEVNQAVLDNKADVAVHSMKDIL
TEISENLEIYAVLPRDPPFDILISRKNLFNLEPNSIIGTSSIRRKNFLTFYRNDLNIKDLRGNIDTRLKKYFEGQYDGII
IAEASIYRLKEQVQYYRLDPHLFTPEANQGIIVVIGRKKDETIKKIFNEINDKDTLEIAIAERKALSIVGGGCHSPIGVY
FEKYDKDFHGIISSSFGRKKITIEEYFHNMTPYEAGELLGKRFLEEIKNEGIIP
>Mature_294_residues
MRIRIAARGSKLSRIQVMMVENYLHKLGIETEFIEIKTKADLFQNEPLSKLGKGVFEKEVNQAVLDNKADVAVHSMKDIL
TEISENLEIYAVLPRDPPFDILISRKNLFNLEPNSIIGTSSIRRKNFLTFYRNDLNIKDLRGNIDTRLKKYFEGQYDGII
IAEASIYRLKEQVQYYRLDPHLFTPEANQGIIVVIGRKKDETIKKIFNEINDKDTLEIAIAERKALSIVGGGCHSPIGVY
FEKYDKDFHGIISSSFGRKKITIEEYFHNMTPYEAGELLGKRFLEEIKNEGIIP

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=288, Percent_Identity=32.6388888888889, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI20149500, Length=288, Percent_Identity=32.6388888888889, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI48994974, Length=297, Percent_Identity=35.3535353535354, Blast_Score=158, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319996, Length=256, Percent_Identity=31.25, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20130425, Length=250, Percent_Identity=32.4, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_SULTO (Q976H1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376067.1
- ProteinModelPortal:   Q976H1
- SMR:   Q976H1
- GeneID:   1458106
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0217
- NMPDR:   fig|273063.1.peg.235
- HOGENOM:   HBG624842
- OMA:   DADILEM
- ProtClustDB:   PRK00072
- BioCyc:   STOK273063:ST0217-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33820; Mature: 33820

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRIAARGSKLSRIQVMMVENYLHKLGIETEFIEIKTKADLFQNEPLSKLGKGVFEKEV
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCHHHHHHHHHHHHH
NQAVLDNKADVAVHSMKDILTEISENLEIYAVLPRDPPFDILISRKNLFNLEPNSIIGTS
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCEECCCCCCEECCC
SIRRKNFLTFYRNDLNIKDLRGNIDTRLKKYFEGQYDGIIIAEASIYRLKEQVQYYRLDP
HHHHHHHHEEEECCCCHHHHCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHEECCC
HLFTPEANQGIIVVIGRKKDETIKKIFNEINDKDTLEIAIAERKALSIVGGGCHSPIGVY
EEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHH
FEKYDKDFHGIISSSFGRKKITIEEYFHNMTPYEAGELLGKRFLEEIKNEGIIP
HHHHCCHHHHHHHCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIRIAARGSKLSRIQVMMVENYLHKLGIETEFIEIKTKADLFQNEPLSKLGKGVFEKEV
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCHHHHHHHHHHHHH
NQAVLDNKADVAVHSMKDILTEISENLEIYAVLPRDPPFDILISRKNLFNLEPNSIIGTS
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCEECCCCCCEECCC
SIRRKNFLTFYRNDLNIKDLRGNIDTRLKKYFEGQYDGIIIAEASIYRLKEQVQYYRLDP
HHHHHHHHEEEECCCCHHHHCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHEECCC
HLFTPEANQGIIVVIGRKKDETIKKIFNEINDKDTLEIAIAERKALSIVGGGCHSPIGVY
EEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHH
FEKYDKDFHGIISSSFGRKKITIEEYFHNMTPYEAGELLGKRFLEEIKNEGIIP
HHHHCCHHHHHHHCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479