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The map label for this gene is nfi [H]

Identifier: 159041809

GI number: 159041809

Start: 1314211

End: 1314897

Strand: Reverse

Name: nfi [H]

Synonym: Cmaq_1244

Alternate gene names: 159041809

Gene position: 1314897-1314211 (Counterclockwise)

Preceding gene: 159041810

Following gene: 159041807

Centisome position: 63.29

GC content: 46.43

Gene sequence:

>687_bases
ATGGTTAGGAGGATTATTAGTGAGAATTACGTTAAGTCAAGGGTACCTAAGGGCTTTAACCTTGAGGCTGCACGCATGGT
TCAGAGGAGGCTGGCGTCAATGGTTGTTACCATGCCATTAACGCAGTCTGTAGACCTCATTACCGGTATTGATGTAGCCT
ACAGGGGTAATTACGCCTACGCAGTGGCGGCAACCTACTCCATGAGGCTTAACCACATTATTGAGTATGGGTGTTATGAG
GGTGAGGTCACTTTCCCATACGTACCAACCCTACTATCCTTTAGGGAACTCTCACCAATGATTAAAGCCTTCATGAGGCT
TAAGGAAAGGCCCCATGTTGTGTTAATTGATGGCCACGGTGTAGCCCACCCTTATAGGCTTGGTATAGCTGCGCACTTCG
GTGTGGTAATGGGTTTACCGACTATTGGGGTTGCTAAATCATTACTCTACGGTGAGGTTAAGGAATCCCTCATAATTGAT
CCAAGTAATGGGGAAGTCATAGGCGGTGTGGTTAAATGCGGGAGGCATGAGGTTTACGTTAGCGTGGGTAACATGATTAC
GCTTGACAATGCCTTAACATTGGTTTCAAGACTGTGCCTAAGGGATAGGATGCCTGAACCAATACTGCATGCTCACACGA
TGGCTAATAAGGTTAAACACAATGGCTGCGTTAATGCATTAGCCTGA

Upstream 100 bases:

>100_bases
CTCATCCCTCAGGAATCATTAAGCCGCGTTAATACTTATACGCTTAATTAGGAAAACCATAAAGCCTACTTAACGTGGGT
TTAATCGGTGAGTTGGCTTA

Downstream 100 bases:

>100_bases
GGGGTGATTCATGTAGAGTACGTAAATGTACCTGTCGCTTAATTCAAAGACATCATGGGGGTAGAGTGAGGCTAATGCCT
TAACCTTATTCCTAATGACA

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MVRRIISENYVKSRVPKGFNLEAARMVQRRLASMVVTMPLTQSVDLITGIDVAYRGNYAYAVAATYSMRLNHIIEYGCYE
GEVTFPYVPTLLSFRELSPMIKAFMRLKERPHVVLIDGHGVAHPYRLGIAAHFGVVMGLPTIGVAKSLLYGEVKESLIID
PSNGEVIGGVVKCGRHEVYVSVGNMITLDNALTLVSRLCLRDRMPEPILHAHTMANKVKHNGCVNALA

Sequences:

>Translated_228_residues
MVRRIISENYVKSRVPKGFNLEAARMVQRRLASMVVTMPLTQSVDLITGIDVAYRGNYAYAVAATYSMRLNHIIEYGCYE
GEVTFPYVPTLLSFRELSPMIKAFMRLKERPHVVLIDGHGVAHPYRLGIAAHFGVVMGLPTIGVAKSLLYGEVKESLIID
PSNGEVIGGVVKCGRHEVYVSVGNMITLDNALTLVSRLCLRDRMPEPILHAHTMANKVKHNGCVNALA
>Mature_228_residues
MVRRIISENYVKSRVPKGFNLEAARMVQRRLASMVVTMPLTQSVDLITGIDVAYRGNYAYAVAATYSMRLNHIIEYGCYE
GEVTFPYVPTLLSFRELSPMIKAFMRLKERPHVVLIDGHGVAHPYRLGIAAHFGVVMGLPTIGVAKSLLYGEVKESLIID
PSNGEVIGGVVKCGRHEVYVSVGNMITLDNALTLVSRLCLRDRMPEPILHAHTMANKVKHNGCVNALA

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=209, Percent_Identity=28.2296650717703, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI87082357, Length=212, Percent_Identity=33.9622641509434, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25223; Mature: 25223

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRIISENYVKSRVPKGFNLEAARMVQRRLASMVVTMPLTQSVDLITGIDVAYRGNYAY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCEEE
AVAATYSMRLNHIIEYGCYEGEVTFPYVPTLLSFRELSPMIKAFMRLKERPHVVLIDGHG
EEEHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
VAHPYRLGIAAHFGVVMGLPTIGVAKSLLYGEVKESLIIDPSNGEVIGGVVKCGRHEVYV
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCEEEHHHHCCCEEEEE
SVGNMITLDNALTLVSRLCLRDRMPEPILHAHTMANKVKHNGCVNALA
EECCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVRRIISENYVKSRVPKGFNLEAARMVQRRLASMVVTMPLTQSVDLITGIDVAYRGNYAY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCEEE
AVAATYSMRLNHIIEYGCYEGEVTFPYVPTLLSFRELSPMIKAFMRLKERPHVVLIDGHG
EEEHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
VAHPYRLGIAAHFGVVMGLPTIGVAKSLLYGEVKESLIIDPSNGEVIGGVVKCGRHEVYV
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCEEEHHHHCCCEEEEE
SVGNMITLDNALTLVSRLCLRDRMPEPILHAHTMANKVKHNGCVNALA
EECCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]