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The map label for this gene is priB [H]

Identifier: 159041402

GI number: 159041402

Start: 870231

End: 871346

Strand: Reverse

Name: priB [H]

Synonym: Cmaq_0829

Alternate gene names: 159041402

Gene position: 871346-870231 (Counterclockwise)

Preceding gene: 159041403

Following gene: 159041401

Centisome position: 41.94

GC content: 44.0

Gene sequence:

>1116_bases
ATGCGTACTGAATCATGCGGAATTAGTGAACTGGTGAGCAAGTATCCATTTACCAGGAGTGGACGAAGCATTATCGAGGA
GGTGGGTATTAGAGTTAGTGAGTTAATGAGCAGGGAGTTTGAGCCAATTGTTAATGATGCATTAAGAATGCTTGAGGCAT
TGATTAAGAATGGTGAACACGCTAAGCTAAACTTTAGTGTGAGGGATGTGGCTGTGTCAAGGTACATAGCCTATGTTCTA
ACAGCCTCAATGGATAAGAGAATTTGGAGGAGAATAGCCGATTCAGAGAGTAAAATATTCTCAAACATGGTTAAGTTGAG
TTACTTAAATAAGCCTAACTTAGAATGCATTAAGGAGGTTGCTAGGGAACTTGAATTAAGGATTATAGGTGTTGGTGAAG
TATCATCATTAGTTGCTAAGCGGCTTAACCTGCAGGGTCAAGTGCTAATGCCTAAGCCATTCATGTACGCGGTCTCCATT
TGGGATTACTTAAGGAATAGCCCTAGGAATGATCCAAACTGGGTCCTGGGTACTAGACCAATTATCCATGGTTACATCCT
CCTAACACAGCATGATGTCTACAGGATTATTGAAGAAGCCGTGGAGAATAAGGCGATTACAATGATAAGTAAACTAGGCA
GTAACCTAGACCTCTTTAAGGAACTCATAGATAGTGTTAATGATAGGCTTAAGGCTATTGAGGAGTCTGCACCCCAGGTT
AGGTATAGGGTTAGTAGGAGGGTTTTACCTAAGGGTGATCCACCATGTGTAACAGCTATAATTAATGAGCTTAAGGCTGG
TGGCAACCCAAGCCACATGGCTAGGTTCACCTTAGCCACCTACATGCTCCACAGGTGTGTGGCTGAGGGTAAGGAGAGGG
AGGAGTGTGTTGATGAGGTTCTAGAGCCCTTTACGCATGCTGCTGATTATAATGAGAAGATAACTAGATACCAGGTGGAG
CACTTGGCTGGCCTTAGGGGTGGTAGGAAATTCTACACCCCACCCTCATGTCAAGAGTTGAATAGCCTAGGGTTATGCCC
AACTAACCTAGGCTGCGGTGTCAAGAATCCCATTCAATTCATACGTGGTTCGACTAAGGTTGAGGGGGCGGAATGA

Upstream 100 bases:

>100_bases
TCTAATTATTATGCTTACCTTAAAATTACCTCTCACTTAGATAATACTTTAAGTTAACTATCCACTGGTTATTAAAGGCG
TTTTAGAGCCACTATAATTA

Downstream 100 bases:

>100_bases
TTGGTCTCATCTCTGTGAAACATAATGTTAATTAATCTCCAGACCTTAAGCTAGCTAATGATGGATGAATTAGATATTAA
AATCCTGGAATCCCTGCAAA

Product: DNA primase, large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRTESCGISELVSKYPFTRSGRSIIEEVGIRVSELMSREFEPIVNDALRMLEALIKNGEHAKLNFSVRDVAVSRYIAYVL
TASMDKRIWRRIADSESKIFSNMVKLSYLNKPNLECIKEVARELELRIIGVGEVSSLVAKRLNLQGQVLMPKPFMYAVSI
WDYLRNSPRNDPNWVLGTRPIIHGYILLTQHDVYRIIEEAVENKAITMISKLGSNLDLFKELIDSVNDRLKAIEESAPQV
RYRVSRRVLPKGDPPCVTAIINELKAGGNPSHMARFTLATYMLHRCVAEGKEREECVDEVLEPFTHAADYNEKITRYQVE
HLAGLRGGRKFYTPPSCQELNSLGLCPTNLGCGVKNPIQFIRGSTKVEGAE

Sequences:

>Translated_371_residues
MRTESCGISELVSKYPFTRSGRSIIEEVGIRVSELMSREFEPIVNDALRMLEALIKNGEHAKLNFSVRDVAVSRYIAYVL
TASMDKRIWRRIADSESKIFSNMVKLSYLNKPNLECIKEVARELELRIIGVGEVSSLVAKRLNLQGQVLMPKPFMYAVSI
WDYLRNSPRNDPNWVLGTRPIIHGYILLTQHDVYRIIEEAVENKAITMISKLGSNLDLFKELIDSVNDRLKAIEESAPQV
RYRVSRRVLPKGDPPCVTAIINELKAGGNPSHMARFTLATYMLHRCVAEGKEREECVDEVLEPFTHAADYNEKITRYQVE
HLAGLRGGRKFYTPPSCQELNSLGLCPTNLGCGVKNPIQFIRGSTKVEGAE
>Mature_371_residues
MRTESCGISELVSKYPFTRSGRSIIEEVGIRVSELMSREFEPIVNDALRMLEALIKNGEHAKLNFSVRDVAVSRYIAYVL
TASMDKRIWRRIADSESKIFSNMVKLSYLNKPNLECIKEVARELELRIIGVGEVSSLVAKRLNLQGQVLMPKPFMYAVSI
WDYLRNSPRNDPNWVLGTRPIIHGYILLTQHDVYRIIEEAVENKAITMISKLGSNLDLFKELIDSVNDRLKAIEESAPQV
RYRVSRRVLPKGDPPCVTAIINELKAGGNPSHMARFTLATYMLHRCVAEGKEREECVDEVLEPFTHAADYNEKITRYQVE
HLAGLRGGRKFYTPPSCQELNSLGLCPTNLGCGVKNPIQFIRGSTKVEGAE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 41988; Mature: 41988

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTESCGISELVSKYPFTRSGRSIIEEVGIRVSELMSREFEPIVNDALRMLEALIKNGEH
CCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
AKLNFSVRDVAVSRYIAYVLTASMDKRIWRRIADSESKIFSNMVKLSYLNKPNLECIKEV
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
ARELELRIIGVGEVSSLVAKRLNLQGQVLMPKPFMYAVSIWDYLRNSPRNDPNWVLGTRP
HHHHCEEEEECCHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCH
IIHGYILLTQHDVYRIIEEAVENKAITMISKLGSNLDLFKELIDSVNDRLKAIEESAPQV
HHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHH
RYRVSRRVLPKGDPPCVTAIINELKAGGNPSHMARFTLATYMLHRCVAEGKEREECVDEV
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LEPFTHAADYNEKITRYQVEHLAGLRGGRKFYTPPSCQELNSLGLCPTNLGCGVKNPIQF
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
IRGSTKVEGAE
HCCCCCCCCCC
>Mature Secondary Structure
MRTESCGISELVSKYPFTRSGRSIIEEVGIRVSELMSREFEPIVNDALRMLEALIKNGEH
CCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
AKLNFSVRDVAVSRYIAYVLTASMDKRIWRRIADSESKIFSNMVKLSYLNKPNLECIKEV
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
ARELELRIIGVGEVSSLVAKRLNLQGQVLMPKPFMYAVSIWDYLRNSPRNDPNWVLGTRP
HHHHCEEEEECCHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCH
IIHGYILLTQHDVYRIIEEAVENKAITMISKLGSNLDLFKELIDSVNDRLKAIEESAPQV
HHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHH
RYRVSRRVLPKGDPPCVTAIINELKAGGNPSHMARFTLATYMLHRCVAEGKEREECVDEV
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LEPFTHAADYNEKITRYQVEHLAGLRGGRKFYTPPSCQELNSLGLCPTNLGCGVKNPIQF
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
IRGSTKVEGAE
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA