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The map label for this gene is aglA [H]

Identifier: 159041403

GI number: 159041403

Start: 871868

End: 873262

Strand: Reverse

Name: aglA [H]

Synonym: Cmaq_0830

Alternate gene names: 159041403

Gene position: 873262-871868 (Counterclockwise)

Preceding gene: 159041405

Following gene: 159041402

Centisome position: 42.03

GC content: 45.16

Gene sequence:

>1395_bases
ATGGCATCTAGGCATGGTTTTAAGATAGCCCTAATAGGGGCTGGTAGTGCGGCGTGGGCTATTGGTCTTATTAAGGACCT
AGCCCTAATACCAAGCTTAAGCGGTAGTACCGTGGTTTTAATGGATATTGATGAGGATAGGTTAGCTTTAGTTAGTAGGT
TTGCCAAGAGGTATGTTTCTGAGGTTAAGGGTAATTTAAACATAGTTACCACCACTGATAGGAGGGAGGCTATTAGGGAT
GCTGACTTCGTGGTTAACTCAACCCTGGCTAAGGGGCATGGGCACTATGAAAGGATGAGGGAGGTTTCTGAGAAGTACGG
GTACTATAGGGGTATTAATAGTGTTGAGTGGAACATGGTGTCTGATTACCACACAATATGGGGCTACTACCAGTTTAAAC
TAGCCCTAGATATAGCTAATGATGTGGTGGATTACGCACCTAATGCATGGTTACTTAACGTATCTAACCCGGTCTTCGAG
TTAACAACATTGATTAGTAGGGAGACTAAGGCTAGGGTTATTGGGTTGTGTGACGGCTACTACGCCTATAGGGATTTACT
CAGGGTTCTTGGTCTCGAGGAGGGTAAGGCTGAGGTTGAGGTTATTGGTGTTAATCATGATGACTGGTTAACTAGGCTTA
AGTACAATGGCGAAGACGCATACCACCTTATTGATGAGTGGATCAGCACTAAGTCCAGTCAATACTTCGAGAAGTGGAGG
GAGGAGCAGAGTAACCCCTTTGATGTTCATGTTTCACCAGTGGCGGTTGACATGTATAGGATGTATGGCCTATGGCCAAT
AGGGGACACCGTTAGGAGTGGTACATGGAAGTACCACTGGGATCTTAAGACCAAGCAATACTGGTATGGGCCACTCGGTG
GACCTGACTCAGAGATTGGGTGGGCCATGTACTTAACGTGGCATAAGATCGAGTTCAATGAGCTTAAGAGGGCGCTTGAG
AATGAGGCTAAGCCATTAACAGACTACATACCGCCAGTTAGAAGTGAGGGTGAGCCGGTTACAATGGTTATTGAGGCTAT
TGTTGAGGATAGTGGTAAGGTAATTGAGGTTAATGTACCTAATCAGGATGCAATACCTGGAATACCCAGTGATGTGGCTG
TTGAAATGCCGGCTAGGGTGGATGCTAAGGGTGTTCATAGATTAAGCTTCAGTAACCTACCTAAGGCGTGGGGTAAGGTG
CTTAAGTACGCTATAATGCCTAGGGTAATTAGGGGTGAGTGGGCGATTGAGGCATTCCTAGGGGGTGGTAGAGACACGTT
ATTTAACTGGCTTATAATTGATCCAAGGACTAAGTCCAGTGATCAAGTCAACCAGGTTATAGATGCAATACTTAAAATAC
CTGGAAATGAGGAAATGGCTAAACACTTCAGTTAA

Upstream 100 bases:

>100_bases
ATTAATTATATATTGATTAATGATTTACTATATAATATTTTTATTATTGTCCTTAATCAATATATAATTAACTTTAAAAG
AACTTAAGTACATTGTATTT

Downstream 100 bases:

>100_bases
TTTACAGTAAAAATATGCCTCATCTTATTCTGGGGTTTTTACTTGAGAGTGCTTATGGAAGAAAAACATGAGTACTACTT
TTGTTTACTCATAGGGTGGT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Maltase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MASRHGFKIALIGAGSAAWAIGLIKDLALIPSLSGSTVVLMDIDEDRLALVSRFAKRYVSEVKGNLNIVTTTDRREAIRD
ADFVVNSTLAKGHGHYERMREVSEKYGYYRGINSVEWNMVSDYHTIWGYYQFKLALDIANDVVDYAPNAWLLNVSNPVFE
LTTLISRETKARVIGLCDGYYAYRDLLRVLGLEEGKAEVEVIGVNHDDWLTRLKYNGEDAYHLIDEWISTKSSQYFEKWR
EEQSNPFDVHVSPVAVDMYRMYGLWPIGDTVRSGTWKYHWDLKTKQYWYGPLGGPDSEIGWAMYLTWHKIEFNELKRALE
NEAKPLTDYIPPVRSEGEPVTMVIEAIVEDSGKVIEVNVPNQDAIPGIPSDVAVEMPARVDAKGVHRLSFSNLPKAWGKV
LKYAIMPRVIRGEWAIEAFLGGGRDTLFNWLIIDPRTKSSDQVNQVIDAILKIPGNEEMAKHFS

Sequences:

>Translated_464_residues
MASRHGFKIALIGAGSAAWAIGLIKDLALIPSLSGSTVVLMDIDEDRLALVSRFAKRYVSEVKGNLNIVTTTDRREAIRD
ADFVVNSTLAKGHGHYERMREVSEKYGYYRGINSVEWNMVSDYHTIWGYYQFKLALDIANDVVDYAPNAWLLNVSNPVFE
LTTLISRETKARVIGLCDGYYAYRDLLRVLGLEEGKAEVEVIGVNHDDWLTRLKYNGEDAYHLIDEWISTKSSQYFEKWR
EEQSNPFDVHVSPVAVDMYRMYGLWPIGDTVRSGTWKYHWDLKTKQYWYGPLGGPDSEIGWAMYLTWHKIEFNELKRALE
NEAKPLTDYIPPVRSEGEPVTMVIEAIVEDSGKVIEVNVPNQDAIPGIPSDVAVEMPARVDAKGVHRLSFSNLPKAWGKV
LKYAIMPRVIRGEWAIEAFLGGGRDTLFNWLIIDPRTKSSDQVNQVIDAILKIPGNEEMAKHFS
>Mature_463_residues
ASRHGFKIALIGAGSAAWAIGLIKDLALIPSLSGSTVVLMDIDEDRLALVSRFAKRYVSEVKGNLNIVTTTDRREAIRDA
DFVVNSTLAKGHGHYERMREVSEKYGYYRGINSVEWNMVSDYHTIWGYYQFKLALDIANDVVDYAPNAWLLNVSNPVFEL
TTLISRETKARVIGLCDGYYAYRDLLRVLGLEEGKAEVEVIGVNHDDWLTRLKYNGEDAYHLIDEWISTKSSQYFEKWRE
EQSNPFDVHVSPVAVDMYRMYGLWPIGDTVRSGTWKYHWDLKTKQYWYGPLGGPDSEIGWAMYLTWHKIEFNELKRALEN
EAKPLTDYIPPVRSEGEPVTMVIEAIVEDSGKVIEVNVPNQDAIPGIPSDVAVEMPARVDAKGVHRLSFSNLPKAWGKVL
KYAIMPRVIRGEWAIEAFLGGGRDTLFNWLIIDPRTKSSDQVNQVIDAILKIPGNEEMAKHFS

Specific function: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=388, Percent_Identity=24.7422680412371, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 52702; Mature: 52570

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRHGFKIALIGAGSAAWAIGLIKDLALIPSLSGSTVVLMDIDEDRLALVSRFAKRYVS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
EVKGNLNIVTTTDRREAIRDADFVVNSTLAKGHGHYERMREVSEKYGYYRGINSVEWNMV
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SDYHTIWGYYQFKLALDIANDVVDYAPNAWLLNVSNPVFELTTLISRETKARVIGLCDGY
CCHHHHCEEEEEEEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCH
YAYRDLLRVLGLEEGKAEVEVIGVNHDDWLTRLKYNGEDAYHLIDEWISTKSSQYFEKWR
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHEEEECCCHHHHHHHHHHCCCCHHHHHHHH
EEQSNPFDVHVSPVAVDMYRMYGLWPIGDTVRSGTWKYHWDLKTKQYWYGPLGGPDSEIG
HHCCCCEEEEECHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCEEEECCCCCCCCCCC
WAMYLTWHKIEFNELKRALENEAKPLTDYIPPVRSEGEPVTMVIEAIVEDSGKVIEVNVP
EEEEEEEEECCHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
NQDAIPGIPSDVAVEMPARVDAKGVHRLSFSNLPKAWGKVLKYAIMPRVIRGEWAIEAFL
CCCCCCCCCCCCEEECCCCCCCCCCEECCHHCCCHHHHHHHHHHHCCHHHCCCCEEEEHH
GGGRDTLFNWLIIDPRTKSSDQVNQVIDAILKIPGNEEMAKHFS
CCCHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
ASRHGFKIALIGAGSAAWAIGLIKDLALIPSLSGSTVVLMDIDEDRLALVSRFAKRYVS
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
EVKGNLNIVTTTDRREAIRDADFVVNSTLAKGHGHYERMREVSEKYGYYRGINSVEWNMV
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SDYHTIWGYYQFKLALDIANDVVDYAPNAWLLNVSNPVFELTTLISRETKARVIGLCDGY
CCHHHHCEEEEEEEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCH
YAYRDLLRVLGLEEGKAEVEVIGVNHDDWLTRLKYNGEDAYHLIDEWISTKSSQYFEKWR
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHEEEECCCHHHHHHHHHHCCCCHHHHHHHH
EEQSNPFDVHVSPVAVDMYRMYGLWPIGDTVRSGTWKYHWDLKTKQYWYGPLGGPDSEIG
HHCCCCEEEEECHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCEEEECCCCCCCCCCC
WAMYLTWHKIEFNELKRALENEAKPLTDYIPPVRSEGEPVTMVIEAIVEDSGKVIEVNVP
EEEEEEEEECCHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
NQDAIPGIPSDVAVEMPARVDAKGVHRLSFSNLPKAWGKVLKYAIMPRVIRGEWAIEAFL
CCCCCCCCCCCCEEECCCCCCCCCCEECCHHCCCHHHHHHHHHHHCCHHHCCCCEEEEHH
GGGRDTLFNWLIIDPRTKSSDQVNQVIDAILKIPGNEEMAKHFS
CCCHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571; 10972187; 12062450; 12588867 [H]