Click here to switch to the map view.

The map label for this gene is tgtA [H]

Identifier: 159040616

GI number: 159040616

Start: 37468

End: 39036

Strand: Reverse

Name: tgtA [H]

Synonym: Cmaq_0024

Alternate gene names: 159040616

Gene position: 39036-37468 (Counterclockwise)

Preceding gene: 159040617

Following gene: 159040611

Centisome position: 1.88

GC content: 42.45

Gene sequence:

>1569_bases
ATGTTTGAGGTAATAGAGAAGGATGAGATAGCTAGGATTGGTAAACTGTACACTAGGCATGGTGTAATAGAGACTCCTGC
ATTATTCCCTGTAATAAACCCAAGTAAGCAGTACGTTGATTTAAGTAGGATTAAGGAGCTTGGTTTCAATCAAATCATAA
CCAACGCCTACATTATAAGGAAAACCTACGGTGACGCCGCCAGGGAGGTTGGGCTTCATGAAATCATAGGCTGGGATGGC
CCATTAATGACTGATTCAGGCGCATATCAAATACTCCAGTACGGTAACATTGAGGTGAGTCCTGATGAGGCCTTAAGGTA
CCAGGTTGAAATAGGTAGTGATATAGGCGTTATACTGGATATACCAACCAGTTACCCTAGGCCAAGGGAGCTTGTGGAGG
TTGAGGTTAATGAAACCATTAGGAGGGCCAGGAGGGCTATACTTCAACTTAAGGAGCTGGATCCTGAACATAGAATGCTT
ATAGTTGGACCAACCCAGGGGGGTGTTTATAGGGATTTACTGGCCTACTCAGCTAGGATGGTTTCTGAATTACCCTTCGA
CATATATGCAATAGGTAGCCCCACTACACTGCTTCAGGCTTATAATTTCACAGGCATAATTAAAATGATACTCACGGTTA
AGTCAATAATACCACCCGGTAAACCAGTACACTTATTCGGTGTCGGCCACCCATTAATACTACCCCTAGCGGTCGCCTTA
GGTATTGATTTATTCGACTCAGCCTCATACATGCTTTACGCCAATGATGATAGAATAATATTAAGCAGTAGGACTGTTAG
GTTAAGTGAATTGGATAAGGATTACGTCCTTGATGGCTGCGGTAAGAGGGCTGGGGAGTTAATGGAGATGAGTAAGGAGG
AGAGGATTAGGTTAATTGCCCAGCATAACCTGTGGACACTGTCAAGGGAGTTAATGGAGATTAAGCAGAGGATTAAGGAG
CATGATATCTGGAGCTACGTGGCTCAGAAGGCTAGGCAGCATCCATCGGTTTATAGGGCTTACTTAACCTTAAGTAATAG
TTCACTATTCCGTAAACTAACCATTAAATTAGCCAGTGGGCTTAAGGTTAATGCAACGCAGTTGAGTATTCTGGATAATA
GTGACTTAGCGAGACCTGAGGTTCAGTGGGCTAGGCGTAGATTAAGGAGGTTGATTAAGGGTATGGGTGTTTTAACCAAT
GTCCTCATTATTATTGGTGACTATGAGGAACCCTTCATAAGGACTCAAATAGCCAATGAAGTTACTAGGTTGGGTGTAAG
GGTTTTCACATATAATGAGGTCTACGGCTTAATCCCAATTGAATTAAGCGACGTATACCCCTTCTCCCAAACGGTACGTG
TGAATGTTAAACCAAGGCCGATTAAATATGAGATAAGGGACTCAGTAATACTGGTGGAGGATAAGTATAGGGATTTGGTT
AAGTTCATTAAGTGCAGTGGTGAGTGCTTCATTATTTACGTTGACTCACTTAAGAATATTAAAGCATACGAGAAGTATGT
GGGATTAGCCTTAATGAAGCGGGTTAATGAGGGCAGTGGAATTAAGTAA

Upstream 100 bases:

>100_bases
AGAACGTTACTGTTACCGAGGAGGGTGTTAAGGGAACTGGATTAGTGGCTAGGAGGGTTAAGGAGATTTACGATGAATAC
GTCCAATCGAGGAAGAGGTA

Downstream 100 bases:

>100_bases
CCGTGGAAGGTGTTTTTAATAACTATAGGCTATTACCACCATAAAGCTTAGCTAATTCTAAGTAAGCCTTATAATTCTCA
ATTATCCTACGTACATCATC

Product: tRNA-guanine transglycosylase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MFEVIEKDEIARIGKLYTRHGVIETPALFPVINPSKQYVDLSRIKELGFNQIITNAYIIRKTYGDAAREVGLHEIIGWDG
PLMTDSGAYQILQYGNIEVSPDEALRYQVEIGSDIGVILDIPTSYPRPRELVEVEVNETIRRARRAILQLKELDPEHRML
IVGPTQGGVYRDLLAYSARMVSELPFDIYAIGSPTTLLQAYNFTGIIKMILTVKSIIPPGKPVHLFGVGHPLILPLAVAL
GIDLFDSASYMLYANDDRIILSSRTVRLSELDKDYVLDGCGKRAGELMEMSKEERIRLIAQHNLWTLSRELMEIKQRIKE
HDIWSYVAQKARQHPSVYRAYLTLSNSSLFRKLTIKLASGLKVNATQLSILDNSDLARPEVQWARRRLRRLIKGMGVLTN
VLIIIGDYEEPFIRTQIANEVTRLGVRVFTYNEVYGLIPIELSDVYPFSQTVRVNVKPRPIKYEIRDSVILVEDKYRDLV
KFIKCSGECFIIYVDSLKNIKAYEKYVGLALMKRVNEGSGIK

Sequences:

>Translated_522_residues
MFEVIEKDEIARIGKLYTRHGVIETPALFPVINPSKQYVDLSRIKELGFNQIITNAYIIRKTYGDAAREVGLHEIIGWDG
PLMTDSGAYQILQYGNIEVSPDEALRYQVEIGSDIGVILDIPTSYPRPRELVEVEVNETIRRARRAILQLKELDPEHRML
IVGPTQGGVYRDLLAYSARMVSELPFDIYAIGSPTTLLQAYNFTGIIKMILTVKSIIPPGKPVHLFGVGHPLILPLAVAL
GIDLFDSASYMLYANDDRIILSSRTVRLSELDKDYVLDGCGKRAGELMEMSKEERIRLIAQHNLWTLSRELMEIKQRIKE
HDIWSYVAQKARQHPSVYRAYLTLSNSSLFRKLTIKLASGLKVNATQLSILDNSDLARPEVQWARRRLRRLIKGMGVLTN
VLIIIGDYEEPFIRTQIANEVTRLGVRVFTYNEVYGLIPIELSDVYPFSQTVRVNVKPRPIKYEIRDSVILVEDKYRDLV
KFIKCSGECFIIYVDSLKNIKAYEKYVGLALMKRVNEGSGIK
>Mature_522_residues
MFEVIEKDEIARIGKLYTRHGVIETPALFPVINPSKQYVDLSRIKELGFNQIITNAYIIRKTYGDAAREVGLHEIIGWDG
PLMTDSGAYQILQYGNIEVSPDEALRYQVEIGSDIGVILDIPTSYPRPRELVEVEVNETIRRARRAILQLKELDPEHRML
IVGPTQGGVYRDLLAYSARMVSELPFDIYAIGSPTTLLQAYNFTGIIKMILTVKSIIPPGKPVHLFGVGHPLILPLAVAL
GIDLFDSASYMLYANDDRIILSSRTVRLSELDKDYVLDGCGKRAGELMEMSKEERIRLIAQHNLWTLSRELMEIKQRIKE
HDIWSYVAQKARQHPSVYRAYLTLSNSSLFRKLTIKLASGLKVNATQLSILDNSDLARPEVQWARRRLRRLIKGMGVLTN
VLIIIGDYEEPFIRTQIANEVTRLGVRVFTYNEVYGLIPIELSDVYPFSQTVRVNVKPRPIKYEIRDSVILVEDKYRDLV
KFIKCSGECFIIYVDSLKNIKAYEKYVGLALMKRVNEGSGIK

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=261, Percent_Identity=27.2030651340996, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=26.0869565217391, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544680, Length=268, Percent_Identity=22.0149253731343, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920510, Length=381, Percent_Identity=25.1968503937008, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 59480; Mature: 59480

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVIEKDEIARIGKLYTRHGVIETPALFPVINPSKQYVDLSRIKELGFNQIITNAYIIR
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHEEHHHHHHCCHHHHHHCEEEEE
KTYGDAAREVGLHEIIGWDGPLMTDSGAYQILQYGNIEVSPDEALRYQVEIGSDIGVILD
ECHHHHHHHCCHHHHHCCCCCEECCCCCEEEEEECCEEECCCHHEEEEEEECCCCEEEEE
IPTSYPRPRELVEVEVNETIRRARRAILQLKELDPEHRMLIVGPTQGGVYRDLLAYSARM
CCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
VSELPFDIYAIGSPTTLLQAYNFTGIIKMILTVKSIIPPGKPVHLFGVGHPLILPLAVAL
HHHCCCEEEECCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
GIDLFDSASYMLYANDDRIILSSRTVRLSELDKDYVLDGCGKRAGELMEMSKEERIRLIA
CCHHCCCCCEEEEECCCEEEEECCEEEHHHCCHHHHHHHCCHHHHHHHHCCHHHHEEEEE
QHNLWTLSRELMEIKQRIKEHDIWSYVAQKARQHPSVYRAYLTLSNSSLFRKLTIKLASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCC
LKVNATQLSILDNSDLARPEVQWARRRLRRLIKGMGVLTNVLIIIGDYEEPFIRTQIANE
CEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHH
VTRLGVRVFTYNEVYGLIPIELSDVYPFSQTVRVNVKPRPIKYEIRDSVILVEDKYRDLV
HHHHCEEEEEECCEEEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCEEEEEHHHHHHH
KFIKCSGECFIIYVDSLKNIKAYEKYVGLALMKRVNEGSGIK
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFEVIEKDEIARIGKLYTRHGVIETPALFPVINPSKQYVDLSRIKELGFNQIITNAYIIR
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHEEHHHHHHCCHHHHHHCEEEEE
KTYGDAAREVGLHEIIGWDGPLMTDSGAYQILQYGNIEVSPDEALRYQVEIGSDIGVILD
ECHHHHHHHCCHHHHHCCCCCEECCCCCEEEEEECCEEECCCHHEEEEEEECCCCEEEEE
IPTSYPRPRELVEVEVNETIRRARRAILQLKELDPEHRMLIVGPTQGGVYRDLLAYSARM
CCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
VSELPFDIYAIGSPTTLLQAYNFTGIIKMILTVKSIIPPGKPVHLFGVGHPLILPLAVAL
HHHCCCEEEECCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
GIDLFDSASYMLYANDDRIILSSRTVRLSELDKDYVLDGCGKRAGELMEMSKEERIRLIA
CCHHCCCCCEEEEECCCEEEEECCEEEHHHCCHHHHHHHCCHHHHHHHHCCHHHHEEEEE
QHNLWTLSRELMEIKQRIKEHDIWSYVAQKARQHPSVYRAYLTLSNSSLFRKLTIKLASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCC
LKVNATQLSILDNSDLARPEVQWARRRLRRLIKGMGVLTNVLIIIGDYEEPFIRTQIANE
CEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHH
VTRLGVRVFTYNEVYGLIPIELSDVYPFSQTVRVNVKPRPIKYEIRDSVILVEDKYRDLV
HHHHCEEEEEECCEEEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCEEEEEHHHHHHH
KFIKCSGECFIIYVDSLKNIKAYEKYVGLALMKRVNEGSGIK
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]