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The map label for this gene is purA [H]

Identifier: 159040611

GI number: 159040611

Start: 31977

End: 32987

Strand: Reverse

Name: purA [H]

Synonym: Cmaq_0019

Alternate gene names: 159040611

Gene position: 32987-31977 (Counterclockwise)

Preceding gene: 159040616

Following gene: 159040610

Centisome position: 1.59

GC content: 47.58

Gene sequence:

>1011_bases
GTGCCTCTTACAGTAGTTGTGGGTGGTTTCTTCGGTGACGAGGGTAAGGGCAAAGTCATCTCATACCTCAGTCTCGTTGA
TAAACCCGACATATGCGTTAGGACAGGTTCAATAAATGCAGGACACACGGTTAATCTAAGGGACCGTACATGGAAGGTTA
GGATAATACCCTCATGCTTCCCTAATGAGGAAACCAGGTTAATGATTGCCCCAGGGGCTTTATTCAGTATTAGTGTCTTA
ATGAGGGAGATTGATGAGACAGGCAGTAGGGGGAGGGTTTGGGTTGACTACTCCTCGGGTATAATTGAGGATAAGCATGT
TGAGGCGGAAAGGAGGGATGAGTACTTAATGAAGACTATTGGTTCAACGGGCCAGGGGGTTGGGGCAGCCATGGTGGATA
GGGTTCTTAGGAGACTTAAGTTGGCTAGGGATTTCGAGGAGTTAAGGGGCATGTTAATTGATGTGCCAAGGGAGGTTAAT
GAATCACTGGAGAGGGGTAGGCTTGTGATTATTGAGGGTACTCAGGGAACATTCCTAAGCCTATACCATGGCACGTACCC
ATACGTCACAAGTAGGGACACCACTGCTTCAGGTATTATTAGTGAGGTTGGTGTTAGCCCAAGGAGTGTGAGTGATATTA
TATTAATCTTTAAGGCATTCGTAAGTAGGGTTGGTGCAGGGGAATTACCTGGTGAGTTAAAGCCTGAGGAGGCGGAGGCT
AGGGGTTGGGTTGAGAAGGGTACTGTGACCGGTAGGTTAAGGAGGGTTGCACCGTTTAACATTGATTTAGCTAAGAGGGC
TGTAATGCTGAATAAACCCACTCAAATAGCCATAACTAAACTCGACGCATTATTCCCTGAGGCGAGGGGGAGGAGGAAGT
GGGGGGAATTACCCACTGAGGCTAGGAAGTGGATAAACAATATTAGTGAAGAGTTGGGTGTGCCGGTTACGTTAATTGGA
ACGGGGGAGGATGCGTTAGACATGGTTGATTTAAGGGGTGAGTCGCAGTGA

Upstream 100 bases:

>100_bases
GGGGTTAATAAATCCTCCTAATCATTTAATTGATTAACTCAATAATGTGTTAATTATTAAACGAAAAACTTAAAAACATG
CTGCATTATTTAATTAAAAA

Downstream 100 bases:

>100_bases
CTTACGACATAGCGATAATTGGGGCTGGACCAGCGGGCTTATTTGCGGCGTATGAACTCACCAGGAATGCTGATGGATTA
AGAATAGCATTAGTGGATAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPLTVVVGGFFGDEGKGKVISYLSLVDKPDICVRTGSINAGHTVNLRDRTWKVRIIPSCFPNEETRLMIAPGALFSISVL
MREIDETGSRGRVWVDYSSGIIEDKHVEAERRDEYLMKTIGSTGQGVGAAMVDRVLRRLKLARDFEELRGMLIDVPREVN
ESLERGRLVIIEGTQGTFLSLYHGTYPYVTSRDTTASGIISEVGVSPRSVSDIILIFKAFVSRVGAGELPGELKPEEAEA
RGWVEKGTVTGRLRRVAPFNIDLAKRAVMLNKPTQIAITKLDALFPEARGRRKWGELPTEARKWINNISEELGVPVTLIG
TGEDALDMVDLRGESQ

Sequences:

>Translated_336_residues
MPLTVVVGGFFGDEGKGKVISYLSLVDKPDICVRTGSINAGHTVNLRDRTWKVRIIPSCFPNEETRLMIAPGALFSISVL
MREIDETGSRGRVWVDYSSGIIEDKHVEAERRDEYLMKTIGSTGQGVGAAMVDRVLRRLKLARDFEELRGMLIDVPREVN
ESLERGRLVIIEGTQGTFLSLYHGTYPYVTSRDTTASGIISEVGVSPRSVSDIILIFKAFVSRVGAGELPGELKPEEAEA
RGWVEKGTVTGRLRRVAPFNIDLAKRAVMLNKPTQIAITKLDALFPEARGRRKWGELPTEARKWINNISEELGVPVTLIG
TGEDALDMVDLRGESQ
>Mature_335_residues
PLTVVVGGFFGDEGKGKVISYLSLVDKPDICVRTGSINAGHTVNLRDRTWKVRIIPSCFPNEETRLMIAPGALFSISVLM
REIDETGSRGRVWVDYSSGIIEDKHVEAERRDEYLMKTIGSTGQGVGAAMVDRVLRRLKLARDFEELRGMLIDVPREVNE
SLERGRLVIIEGTQGTFLSLYHGTYPYVTSRDTTASGIISEVGVSPRSVSDIILIFKAFVSRVGAGELPGELKPEEAEAR
GWVEKGTVTGRLRRVAPFNIDLAKRAVMLNKPTQIAITKLDALFPEARGRRKWGELPTEARKWINNISEELGVPVTLIGT
GEDALDMVDLRGESQ

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=26.6355140186916, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=25.3521126760563, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI40316944, Length=393, Percent_Identity=23.6641221374046, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=30.6603773584906, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25146553, Length=418, Percent_Identity=26.7942583732057, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146555, Length=418, Percent_Identity=26.7942583732057, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324109, Length=422, Percent_Identity=26.303317535545, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=25.3554502369668, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 37140; Mature: 37009

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTVVVGGFFGDEGKGKVISYLSLVDKPDICVRTGSINAGHTVNLRDRTWKVRIIPSCF
CCEEEEEECEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEEECCCCC
PNEETRLMIAPGALFSISVLMREIDETGSRGRVWVDYSSGIIEDKHVEAERRDEYLMKTI
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHH
GSTGQGVGAAMVDRVLRRLKLARDFEELRGMLIDVPREVNESLERGRLVIIEGTQGTFLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCEEEEECCCCCEEE
LYHGTYPYVTSRDTTASGIISEVGVSPRSVSDIILIFKAFVSRVGAGELPGELKPEEAEA
EECCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RGWVEKGTVTGRLRRVAPFNIDLAKRAVMLNKPTQIAITKLDALFPEARGRRKWGELPTE
CCCHHCCCHHHHEEEECCCCCHHHHHHHEECCCCEEEEEHHHHHCCHHCCCCCCCCCCHH
ARKWINNISEELGVPVTLIGTGEDALDMVDLRGESQ
HHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCC
>Mature Secondary Structure 
PLTVVVGGFFGDEGKGKVISYLSLVDKPDICVRTGSINAGHTVNLRDRTWKVRIIPSCF
CEEEEEECEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEEECCCCC
PNEETRLMIAPGALFSISVLMREIDETGSRGRVWVDYSSGIIEDKHVEAERRDEYLMKTI
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHH
GSTGQGVGAAMVDRVLRRLKLARDFEELRGMLIDVPREVNESLERGRLVIIEGTQGTFLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCEEEEECCCCCEEE
LYHGTYPYVTSRDTTASGIISEVGVSPRSVSDIILIFKAFVSRVGAGELPGELKPEEAEA
EECCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RGWVEKGTVTGRLRRVAPFNIDLAKRAVMLNKPTQIAITKLDALFPEARGRRKWGELPTE
CCCHHCCCHHHHEEEECCCCCHHHHHHHEECCCCEEEEEHHHHHCCHHCCCCCCCCCCHH
ARKWINNISEELGVPVTLIGTGEDALDMVDLRGESQ
HHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]