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The map label for this gene is nadE [H]

Identifier: 15898952

GI number: 15898952

Start: 1996402

End: 1997316

Strand: Direct

Name: nadE [H]

Synonym: SSO2172

Alternate gene names: 15898952

Gene position: 1996402-1997316 (Clockwise)

Preceding gene: 15898951

Following gene: 15898953

Centisome position: 66.72

GC content: 34.75

Gene sequence:

>915_bases
ATGGCCTTTTCTAAATTCTCTTCATTCAGATGGTATTATACGTTAAATATTTGCTCAACAAATATTCTCATAATGGTAAT
GCATGAATATATCAGAAAATCACTCACAATAGATTGTGAAGCTGTTACTAACTATATTGTGGAAAGGATAAGGGAGTATC
TTGAATTTAGCAATAAGAAAGGAGGAGTAATAGGGGTAAGCGGGGGAGTGGATTCAGCGGTAACTGCAACGCTTCTTGCT
AAAGCTACTGATAATTTCTTCATACTTCTCATGCCTTCCTCTTCAACGCCAAAGATAGATTTGGATGATTCATTCGAAAT
GATAAAATTCCTTAATGCACAAAATAAATACAAATTAATTAATATAGATGAAATTGTGAAGTCATTTTCAAATAAAATAG
AAACAGAAAATAAATATATAATAGGCAACATAAAAGCTAGAGTTAGAATGATAATACTTTACGCTTATGCGCAAATGTTA
GATTACTTGGTGGTAGGAACTGGAGACAAGAGTGAGCTACTATTAGGATACTTCACAAAGTATGGGGATGGAGGTGTTGA
TGTTTTACCAATAGGTGATTTGTACAAGACACAGGTTAGAATGCTAGGAAAATGTTTAGGGTTACCGGAAAGAATAGTCA
CAAAACCAAGTTCCCCAGCCTTATGGGAAGGACAAACAGCAGAAGGAGAATTAGGAATTGACTATGAAACAATAGATTCA
ATACTATATTTAAGATTCGATGAAATGAGAAGCGAAGACGAGATAGTGAAAATGTTGGGAATTCCTATAGATATTGTTAA
AAAAGTTGATAGACTAGTTAAAATCTCTCAACACAAGAGGCTACCCCCAGAGATCTTTAGGTTAAGTGGAAGGGCAATAA
ACTCAGACTGGAGGTTCCCTAGAAGATGGGCATAA

Upstream 100 bases:

>100_bases
CCATGGGAGGAAGAATCTACGTAATTACCGTTGAAAATAGGGATTCGATAAAGTACGAGATTTTGAATTACATATTAAGT
AGCCTAGATTAACTTCTTCT

Downstream 100 bases:

>100_bases
AAGTGGAATTAGCCCAAATAAGATCTTATCCCGGGGACGTCTATAGGAACTACAAGAAACATTTAGAAATAATAGAGAGC
AGTAGCGCAGATTGTGTAAT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAFSKFSSFRWYYTLNICSTNILIMVMHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKKGGVIGVSGGVDSAVTATLLA
KATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLINIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQML
DYLVVGTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDS
ILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFPRRWA

Sequences:

>Translated_304_residues
MAFSKFSSFRWYYTLNICSTNILIMVMHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKKGGVIGVSGGVDSAVTATLLA
KATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLINIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQML
DYLVVGTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDS
ILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFPRRWA
>Mature_303_residues
AFSKFSSFRWYYTLNICSTNILIMVMHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKKGGVIGVSGGVDSAVTATLLAK
ATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLINIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQMLD
YLVVGTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDSI
LYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFPRRWA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=282, Percent_Identity=26.9503546099291, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 34712; Mature: 34581

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSKFSSFRWYYTLNICSTNILIMVMHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKK
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCC
GGVIGVSGGVDSAVTATLLAKATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLI
CCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCEEEE
NIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQMLDYLVVGTGDKSELLLGYFTK
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
YGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDS
CCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFP
HHEEHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCCCCC
RRWA
CCCC
>Mature Secondary Structure 
AFSKFSSFRWYYTLNICSTNILIMVMHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKK
CCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCC
GGVIGVSGGVDSAVTATLLAKATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLI
CCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCEEEE
NIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQMLDYLVVGTGDKSELLLGYFTK
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
YGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDS
CCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
ILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFP
HHEEHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCCCCC
RRWA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]