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The map label for this gene is nadE2 [H]

Identifier: 15898953

GI number: 15898953

Start: 1997307

End: 1998119

Strand: Direct

Name: nadE2 [H]

Synonym: SSO2174

Alternate gene names: 15898953

Gene position: 1997307-1998119 (Clockwise)

Preceding gene: 15898952

Following gene: 15898954

Centisome position: 66.75

GC content: 36.78

Gene sequence:

>813_bases
ATGGGCATAAAAGTGGAATTAGCCCAAATAAGATCTTATCCCGGGGACGTCTATAGGAACTACAAGAAACATTTAGAAAT
AATAGAGAGCAGTAGCGCAGATTGTGTAATCTTTCCAGAACTGTCCTTAACTGGTTACATTATAAAGGACTTAACATATG
AAATATACAAAGACGCAGAGGAGGCTACACAGAAAATAGCAGAGAAAGTTAACAAGTGTGTGGTGTTTGGAACAATAAGG
CAAGTTAGGAAAGGAATATTAAGAAACTCAGCAGCAGTTATCATTAACGGGAAATTGGATTACATATATAAGTTTTATCT
TCCAACATATGGATTATTTGAGGAGAGGAGATATTTCCAAAGAGGAGATCCACTGAAAGATCTGAAAATTTTTGAGTACA
AAGACTTAAAATTTGGAGTAGTAATTTGTGAGGATGCTTGGCATCCGGAACCAATAGAGGCGTTATCGCTTATGGGAGCT
GACGCAATTTTCATACCCTCAGCCTCACCAATGCGAAAACTAAGAGAAAACTTGGCAATAGAAGAGAGTTGGGACTCGCT
TTTGAAAGCTCATTCATTAATGAATACTGTATGGACTGTTTTCACAAATGTTGTAGGTAGTCAAGAGGAGGAGTACTTTT
GGGGAGGATCTAGAGTAGTTTCGCCATTAGGAGATGTAAAACTTAAACTAAAACTATTTTATGAGGATAGGGGAACTATA
GAGATCACTGAAAATGAATTGCTTAGAGCTCGGTTCTTTAGTAGTTATAGGGATCATATAAGGGAGTTCCATTCAATTCT
TGATAAACTATAA

Upstream 100 bases:

>100_bases
TTGATAGACTAGTTAAAATCTCTCAACACAAGAGGCTACCCCCAGAGATCTTTAGGTTAAGTGGAAGGGCAATAAACTCA
GACTGGAGGTTCCCTAGAAG

Downstream 100 bases:

>100_bases
CTTTTATAACTCTACATACGATATACATAATTGAATATGATTTCTCAAAGGTTTAATCTCAATCTCTTACTTTTACTTAT
AATTATAATTCTGCTCATCA

Product: amidohydrolase, putative

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MGIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAEEATQKIAEKVNKCVVFGTIR
QVRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQRGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGA
DAIFIPSASPMRKLRENLAIEESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTI
EITENELLRARFFSSYRDHIREFHSILDKL

Sequences:

>Translated_270_residues
MGIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAEEATQKIAEKVNKCVVFGTIR
QVRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQRGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGA
DAIFIPSASPMRKLRENLAIEESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTI
EITENELLRARFFSSYRDHIREFHSILDKL
>Mature_269_residues
GIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAEEATQKIAEKVNKCVVFGTIRQ
VRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQRGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGAD
AIFIPSASPMRKLRENLAIEESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTIE
ITENELLRARFFSSYRDHIREFHSILDKL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31320; Mature: 31189

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAE
CCCEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCHH
EATQKIAEKVNKCVVFGTIRQVRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQ
HHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCEEEEECCHHEEEEECCCCCCHHHHHHHHH
RGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGADAIFIPSASPMRKLRENLAI
CCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCH
EESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCEEEEEEEEEECCCEE
EITENELLRARFFSSYRDHIREFHSILDKL
EECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAE
CCEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCHH
EATQKIAEKVNKCVVFGTIRQVRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQ
HHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCEEEEECCHHEEEEECCCCCCHHHHHHHHH
RGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGADAIFIPSASPMRKLRENLAI
CCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCCH
EESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCEEEEEEEEEECCCEE
EITENELLRARFFSSYRDHIREFHSILDKL
EECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]