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The map label for this gene is pdhA-1 [H]

Identifier: 15898208

GI number: 15898208

Start: 1203909

End: 1204946

Strand: Direct

Name: pdhA-1 [H]

Synonym: SSO1369

Alternate gene names: 15898208

Gene position: 1203909-1204946 (Clockwise)

Preceding gene: 15898207

Following gene: 15898209

Centisome position: 40.23

GC content: 40.27

Gene sequence:

>1038_bases
ATGATTATAAAACCCGAACCATCACTTAAAGATTTATCCTATATAATTGAGAACGCAGGATTAAAGGCTAGTGATTTACT
AAACATGTATAAGAGAATGCTAATTATCAGATACTTCGAAGAGTCCATAAGGAAAATTTACCATGAGGGAAAGAATCCCT
TCAATATGGCATCCGGTAGAATTAGGGGAGAGATGCACCTATCAATAGGGCAAGAGGCAGTTGCAGTTGGTACGTTATAC
GATATTAGAGACGAAGATGTAGTAGTGAGCACCCACAGACCCCATCATCACGCTATTGCCAAAGGCGTTGACTTAAAGGG
ATTGGCAGCGGAAATTTTAGGTAAAGCTACTGGACTCTGCAAAGGAAAAGGAGGGCACATGCATCTGTTCGATAAATCTA
AGAACTTTGCATGTAGCGGAATTGTAGGAGCTTCTTTTCCTCAAGCAGCTGGCGCTGCCTTTGCGTTTAAATATCTAGGA
AAAGATAACGTGGCAATATCATTTGCCGGTGAAGGTGCAGCCAATCATGGTACCTTCGCTGAAACTCTAAATATAGCAAG
TGCTTGGGAATTACCATTAATAATAGTTATAGAAGACAACAAATACGCTGATTCTACACCTAAATCCTTCGTTATGTCCA
CCACTTTCCATTATCAGAGAGGTTTAGCATATAACGTCCCATCATATCTTGTTGACGGAATGGATGTAATAGACGTCTAC
TCAACTTCTAAAAAAGCCATAGAGAGAGCAAGGAAAGGTTTTGGTCCCACATTAATTGAAGCTTTAACTTATAGGTATGT
AGGACATTTTGAAGGAGACGGAGAGGAATATAGAACTAAAGAGGAAGTGGAGTTCTGGAGCTCATTAGATCCTATAAGGA
GATTAGAAAACAGACTATTACGTCTAAATTACGCTGACAGCGACATTCTAGCTAGACTCAGAGAGGAGGCAAGAAAGCAA
GTTCAAGAGGCAATAGATTTCGCAATAAATAGTAAGTACCCAGAGCTAACTGACGCATTCGGAGGTGTCTTCGCATGA

Upstream 100 bases:

>100_bases
ATTACGAAATAATATATACAATTAGACTCAATAATATTCAAAAATACTATAAAGTAAATTATAGAAAGATTTATTAGGTA
AATAATCTATATTAACAAAC

Downstream 100 bases:

>100_bases
AAATAAGGGGAATCGCACAAGCCATTGCCGAGGGAATAAGACAAGAGATGGAGAGAAACGACAGAATTGTGGTACTGGGA
GAAGACGTAACATATTGGGG

Product: pyruvate dehydrogenase alpha subunit (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGRIRGEMHLSIGQEAVAVGTLY
DIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLCKGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLG
KDNVAISFAGEGAANHGTFAETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVY
STSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLLRLNYADSDILARLREEARKQ
VQEAIDFAINSKYPELTDAFGGVFA

Sequences:

>Translated_345_residues
MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGRIRGEMHLSIGQEAVAVGTLY
DIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLCKGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLG
KDNVAISFAGEGAANHGTFAETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVY
STSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLLRLNYADSDILARLREEARKQ
VQEAIDFAINSKYPELTDAFGGVFA
>Mature_345_residues
MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGRIRGEMHLSIGQEAVAVGTLY
DIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLCKGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLG
KDNVAISFAGEGAANHGTFAETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVY
STSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLLRLNYADSDILARLREEARKQ
VQEAIDFAINSKYPELTDAFGGVFA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=37.6947040498442, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI4505685, Length=321, Percent_Identity=37.3831775700935, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=37.3831775700935, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=36.5853658536585, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI291084757, Length=321, Percent_Identity=32.398753894081, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI11386135, Length=332, Percent_Identity=30.7228915662651, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI258645172, Length=337, Percent_Identity=30.5637982195846, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564172, Length=313, Percent_Identity=37.3801916932907, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17536047, Length=313, Percent_Identity=37.3801916932907, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI86563355, Length=323, Percent_Identity=28.7925696594427, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI86563357, Length=323, Percent_Identity=28.7925696594427, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321026, Length=337, Percent_Identity=36.7952522255193, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24639748, Length=327, Percent_Identity=37.3088685015291, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24639744, Length=311, Percent_Identity=37.620578778135, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28571106, Length=311, Percent_Identity=37.620578778135, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24639740, Length=311, Percent_Identity=37.620578778135, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24639746, Length=299, Percent_Identity=38.1270903010033, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=27.2445820433437, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 38371; Mature: 38371

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IRGEMHLSIGQEAVAVGTLYDIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLC
EEEEEEEECCCHHHEEEEEEECCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCC
KGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLGKDNVAISFAGEGAANHGTFA
CCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHH
ETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVY
HHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHH
STSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLL
HCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHE
RLNYADSDILARLREEARKQVQEAIDFAINSKYPELTDAFGGVFA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IRGEMHLSIGQEAVAVGTLYDIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLC
EEEEEEEECCCHHHEEEEEEECCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCC
KGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLGKDNVAISFAGEGAANHGTFA
CCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHH
ETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVY
HHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHH
STSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLL
HCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHE
RLNYADSDILARLREEARKQVQEAIDFAINSKYPELTDAFGGVFA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]