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The map label for this gene is pdhB-1 [H]

Identifier: 15898209

GI number: 15898209

Start: 1204943

End: 1205941

Strand: Direct

Name: pdhB-1 [H]

Synonym: SSO1370

Alternate gene names: 15898209

Gene position: 1204943-1205941 (Clockwise)

Preceding gene: 15898208

Following gene: 15898213

Centisome position: 40.27

GC content: 41.14

Gene sequence:

>999_bases
ATGAAAATAAGGGGAATCGCACAAGCCATTGCCGAGGGAATAAGACAAGAGATGGAGAGAAACGACAGAATTGTGGTACT
GGGAGAAGACGTAACATATTGGGGGGCAGTCTTCGGATTTACTATGGGACTTTTTGATAAGTTTGGAAGAAAAAGGGTTA
TCGATACACCCATTACTGAACAAACATTTATGGGCATTAGCGTTGGCGCTGCTTCCTCTGGCTTACACCCAGTTGTTTCA
TTAATGTTCGTAGACTTTCTAGGTGCGGGATTCGATCAAATGTTCAACCATATGGCAAAGAATTATTACATGAGTGGAGG
TCAATATCCCATGCCAATTACTGTAATTACGGCAATAGGAGGAGGTTATGGTGATTCCTCACAGCACTCACAAGTTTTAT
ATTCACTCTTCGCCCACTTACCAGGATTTAAGGTGATAGTACCTTCAACACCATATGACGCTAAAGGTCTTACAATTAAG
GCACTAAGAGACAACAACCCAGTCATAATATTCGGACATAAACTATTAACTGGACTACCATTTTTACCATTTGAAGGGAA
TGAAGAAGAGGTCCCAGAAGAACCTTATGAGATCGAATTCGGCAAAGCAGCCATCAGAAAAGAAGGAACTGATCTAACCA
TAATTTCCGCCGGCTTAATGGTCCATAGAAGCTTGAAGGCTGCGGAAATGCTACAGAAAGAGGGAATTTCAGCCGAAGTA
ATAGACGTAAGAACGTTCGTCCCGTTAGATGAAGAAACCATAATAAAGTCAGCTAGGAAAACCGGAAGAGTGCTAATTGT
AGACGAAGACTATATGAGCTATGGTGTAACTGGAGAGATAGCATTCAGAATACAGTCTAAGGCATTAAAGGATCTTAAAG
TCCCCATATCCAGGCTTGCAGTTCCAGACGTTCCAATACCCTTTTCAGAGCCTTTAGAAAATGCGGTAATACCCAACGTA
AATACAATCTACAGTGAAGCGAAAAAGTTAATCCAATAA

Upstream 100 bases:

>100_bases
GACTCAGAGAGGAGGCAAGAAAGCAAGTTCAAGAGGCAATAGATTTCGCAATAAATAGTAAGTACCCAGAGCTAACTGAC
GCATTCGGAGGTGTCTTCGC

Downstream 100 bases:

>100_bases
ACACTCTAACGAGAAATTATTGAAACTGTTTAGTGGTAGCGTCAAATATTATCAGTAGAATCCGTTTTATACAATAATTT
TCTCTTAATAAATGAACAAT

Product: pyruvate dehydrogenase beta subunit (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITEQTFMGISVGAASSGLHPVVS
LMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIGGGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIK
ALRDNNPVIIFGHKLLTGLPFLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEV
IDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLAVPDVPIPFSEPLENAVIPNV
NTIYSEAKKLIQ

Sequences:

>Translated_332_residues
MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITEQTFMGISVGAASSGLHPVVS
LMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIGGGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIK
ALRDNNPVIIFGHKLLTGLPFLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEV
IDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLAVPDVPIPFSEPLENAVIPNV
NTIYSEAKKLIQ
>Mature_332_residues
MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITEQTFMGISVGAASSGLHPVVS
LMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIGGGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIK
ALRDNNPVIIFGHKLLTGLPFLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEV
IDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLAVPDVPIPFSEPLENAVIPNV
NTIYSEAKKLIQ

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=329, Percent_Identity=39.8176291793313, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI291084858, Length=329, Percent_Identity=37.9939209726444, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=36.697247706422, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=36.697247706422, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI205277463, Length=301, Percent_Identity=27.2425249169435, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4507521, Length=301, Percent_Identity=27.2425249169435, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI225637461, Length=292, Percent_Identity=28.4246575342466, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI225637463, Length=292, Percent_Identity=28.0821917808219, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI225637459, Length=292, Percent_Identity=28.0821917808219, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI133778974, Length=293, Percent_Identity=27.6450511945392, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538422, Length=325, Percent_Identity=37.8461538461538, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17506935, Length=333, Percent_Identity=37.2372372372372, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17539652, Length=266, Percent_Identity=29.6992481203008, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319698, Length=335, Percent_Identity=38.2089552238806, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=37.6146788990826, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=37.6146788990826, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=36.2229102167183, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=36.2229102167183, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24650943, Length=114, Percent_Identity=38.5964912280702, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24650945, Length=114, Percent_Identity=38.5964912280702, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645119, Length=299, Percent_Identity=25.752508361204, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45551847, Length=299, Percent_Identity=25.752508361204, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550715, Length=299, Percent_Identity=25.752508361204, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36566; Mature: 36566

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITE
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEECCCCCCH
QTFMGISVGAASSGLHPVVSLMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIG
HHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIKALRDNNPVIIFGHKLLTGLP
CCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCEEEECCHHHHCCC
FLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEV
CCCCCCCCCCCCCCCCEEECCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEE
IDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLA
EEEEEECCCCHHHHHHHHHHCCCEEEEECHHHHHCCCEEEEEEEHHHHHHHHHCCHHHHC
VPDVPIPFSEPLENAVIPNVNTIYSEAKKLIQ
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITE
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEECCCCCCH
QTFMGISVGAASSGLHPVVSLMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIG
HHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIKALRDNNPVIIFGHKLLTGLP
CCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCEEEECCHHHHCCC
FLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEV
CCCCCCCCCCCCCCCCEEECCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEE
IDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLA
EEEEEECCCCHHHHHHHHHHCCCEEEEECHHHHHCCCEEEEEEEHHHHHHHHHCCHHHHC
VPDVPIPFSEPLENAVIPNVNTIYSEAKKLIQ
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]