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The map label for this gene is purD [H]

Identifier: 15897548

GI number: 15897548

Start: 549129

End: 550580

Strand: Direct

Name: purD [H]

Synonym: SSO0635

Alternate gene names: 15897548

Gene position: 549129-550580 (Clockwise)

Preceding gene: 15897547

Following gene: 15897549

Centisome position: 18.35

GC content: 37.74

Gene sequence:

>1452_bases
ATGGTGAGAAAAATGAAAGTATTACTCGTTGGAGATGGAGCTAGAGAAAACGTTCTAGCCTATTCGTTGGCAAGATCATC
TAAAGGTTACAAGATTTACGCACTATCGTCATATATAAATCCCGGGATTAATTCAATAGTGAAAACCACTGGTGCAGAGT
ATTTTATAGGTAACGTAAACTCCCCAGAAGTTATTAAGGAGGTAATTAAGAAAGTAAACCCAGATTTAGGCGTAATTGGA
CCAGAAGATCCCTTATTCAACGGAATTGCGGACATTTTTAGAAAAGAGGGAATATCGGTATTCGGGGCTAGCAAAAAGTG
TGCAAGGATAGAGGAGTCTAAGGCATGGGCAAGAGAGTTAATGTGGAAACATTCTATTCCAGGAAGATTAAGATATAAGG
TATTTTACACAATAGAAGATACTGCAAAGTTCATATTAGAATATGGCGGATCAGTCGCAATAAAACCTGCTGGGCAAGCT
GGAGGAAAAGGGGTTAAGGTAATAGCTGATCTAGAGGCTTATTTAACCCATGATAAGAGAGAGGCACTGACAAAAAGCGT
GAATGAAATAGGGAGTCTATACAATAAGGAAGGTGAGCCGAGAATTATAATAGAGGAGAAAGTTGATGGACCAGAATACA
CACTTCATGTTTTAAGTGATGGGAAAACAACTATCTCCTTACCTTTGGCTCAAGATTATAAGAACGCGTATCAAGACGGA
ATAGGTCCAGAGACTGGAGGAATGGGATCAATTTCTGGACCTAACGAATTGCTTCCATTTATCAGCAATGAAGAGTATCA
AACAACTTATGATATAGTTAAAAGGACTATGGATGCGATATACAAGGAGACTGGAGAGAGATACGTAGGAGTTATTGCAG
GACAAATGATGTTAACTGAACTTTGGGGACCTACAGTAATTGAGTATTATTCAAGATTTGGTGATCCAGAAGCTTCCGCC
ATAATTCCAAGATTAGAATCTGATTTTGGAGAGACAATTGAGCTCACAGCTACTGGACATTTGAATAAAGCTAGTATAAA
AATAAACGAGAAACCTTCTATAGTCAGAGCTGTTGCTACATTAGGATACCCTATCTCAAAACAAATGGCATCTGGGCATA
AGATTGTAGTAGATTTAGAAAAGATGAAAGAGCGCGGATGCGTGGTATTTTTTGGATCTGTAGCATTAGAGGGAATGCAA
CTTATAACTAAAGGCTCTAGAGCTTTAGAAATAGTTGCAATAGGAGATTTCGAAGAAGCTGCTGAGAACTTAGACAGATG
TATGCAATATATTAGCAGTGATACTAAATTGATATATAGGACTGATATTGGGAGGACAGTTAAATCTCAAATTGAAAAGG
CTGAAATCATAAGATATTCTTATAAAAATAGAGAAAAAAGAGGGATTCTTGGAGTTTCTGCAGATTGGTCTCCTAATGGT
GGGTTATGGTGA

Upstream 100 bases:

>100_bases
TTTTAGGTGCAGACTCTATACACTGGTTAAGCATAGAAGGATTGTTCAAAGCCATTGGGCATAGGGATCTCTGCTTAGGA
TGTATGACTAAAAAATATCC

Downstream 100 bases:

>100_bases
GTGAGGAGTATAAAAAAGCAGGAGTTGATTTAGAAAAATTACGTAATTATCATAATATGATTTCTCAAATAATCTCATCT
ACTTATAAAAATACTATTAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVNSPEVIKEVIKKVNPDLGVIG
PEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWARELMWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQA
GGKGVKVIADLEAYLTHDKREALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDG
IGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTELWGPTVIEYYSRFGDPEASA
IIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVATLGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQ
LITKGSRALEIVAIGDFEEAAENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNG
GLW

Sequences:

>Translated_483_residues
MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVNSPEVIKEVIKKVNPDLGVIG
PEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWARELMWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQA
GGKGVKVIADLEAYLTHDKREALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDG
IGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTELWGPTVIEYYSRFGDPEASA
IIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVATLGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQ
LITKGSRALEIVAIGDFEEAAENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNG
GLW
>Mature_483_residues
MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVNSPEVIKEVIKKVNPDLGVIG
PEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWARELMWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQA
GGKGVKVIADLEAYLTHDKREALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDG
IGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTELWGPTVIEYYSRFGDPEASA
IIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVATLGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQ
LITKGSRALEIVAIGDFEEAAENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNG
GLW

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=460, Percent_Identity=30, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI209869995, Length=460, Percent_Identity=30, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI209869993, Length=460, Percent_Identity=30, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI28416899, Length=445, Percent_Identity=30.7865168539326, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1790438, Length=443, Percent_Identity=29.7968397291196, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17567511, Length=461, Percent_Identity=27.765726681128, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321203, Length=465, Percent_Identity=31.3978494623656, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24582400, Length=465, Percent_Identity=32.258064516129, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI62471619, Length=447, Percent_Identity=32.4384787472036, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 53334; Mature: 53334

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVN
CCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCC
SPEVIKEVIKKVNPDLGVIGPEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWAREL
CHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
MWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQAGGKGVKVIADLEAYLTHDKR
HHHCCCCCEEEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHCCHHH
EALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDG
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCHHHHHHHHHCC
IGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTE
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
LWGPTVIEYYSRFGDPEASAIIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVAT
HCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHH
LGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQLITKGSRALEIVAIGDFEEA
HCCCHHHHHCCCCEEEEEHHHHHHCCEEEEEHHHHHHHHHHHHCCCCEEEEEEECCHHHH
AENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNG
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
GLW
CCC
>Mature Secondary Structure
MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVN
CCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCC
SPEVIKEVIKKVNPDLGVIGPEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWAREL
CHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
MWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQAGGKGVKVIADLEAYLTHDKR
HHHCCCCCEEEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHCCHHH
EALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDG
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCHHHHHHHHHCC
IGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTE
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
LWGPTVIEYYSRFGDPEASAIIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVAT
HCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHH
LGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQLITKGSRALEIVAIGDFEEA
HCCCHHHHHCCCCEEEEEHHHHHHCCEEEEEHHHHHHHHHHHHCCCCEEEEEEECCHHHH
AENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNG
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
GLW
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]