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The map label for this gene is pyrE

Identifier: 15897531

GI number: 15897531

Start: 535932

End: 536519

Strand: Direct

Name: pyrE

Synonym: SSO0615

Alternate gene names: 15897531

Gene position: 535932-536519 (Clockwise)

Preceding gene: 15897519

Following gene: 15897532

Centisome position: 17.91

GC content: 33.16

Gene sequence:

>588_bases
ATGAATTTCGCAGAAGTCTTACTCGAAAGGAAATTATTATTAATAGGAAGTTTCGTTTTAACATCAGGTAAGGTTAGTCC
ATATTACTTAGACTTAAGACCTTTACCAAATTATCCAGAATTTTACGATATAGTTAATCAAGCTATAAAGAAAGCAAAAG
ATATACCCCATGATATAATAGTAGGAATAGCCACTGGAGGAGTTCCCTTATCGGCATTCATAGCTTGTAACCTTAAAGAG
CCTATGGGATATATTAGAATAGAAAAGAAAGGTCATGGAACTAATCGTACATTAGAACTCGATGTAAAAGGAAAAAGAGT
ATTGTTAGTAGATGACGTTGCAACTACAGGAGTATCCATAGAGAAAGCAACATTGGAGATTCTTAACGGTGGAGGTAAAG
TTTCAGACGCACTAGTAATCATAGATAGACAAGAAGGGGCTTCACAAAGATTGGAAAAACTAGGAGTCAAATTACACTCT
CTATTTAAAATTTCAGAAATTCTAGATGAATTGTTGAAGAGTGATAAACTTAAGGATAATGAAAAGAAGTCAATTCTAGA
TTATTTGGTGAAGAATGTTGAAAAGTAG

Upstream 100 bases:

>100_bases
TCAAGTCAAGCGACGAAACTACGTGTCTTAATCTCACAAAGCCCTTATAACTGGTTATGATAGAAGTATATTTAAATTCT
TTTTCACAGAGTCTCTACGT

Downstream 100 bases:

>100_bases
AGTAATATTAGCAATGGATAAACCTCTCTCATATCAAGTTCTTAAAGAGATGGAAAATGAGTTATATGGGATAAAAGTTG
GTTTACCTTTAGTTTTAGAT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDIIVGIATGGVPLSAFIACNLKE
PMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSIEKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHS
LFKISEILDELLKSDKLKDNEKKSILDYLVKNVEK

Sequences:

>Translated_195_residues
MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDIIVGIATGGVPLSAFIACNLKE
PMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSIEKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHS
LFKISEILDELLKSDKLKDNEKKSILDYLVKNVEK
>Mature_195_residues
MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDIIVGIATGGVPLSAFIACNLKE
PMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSIEKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHS
LFKISEILDELLKSDKLKDNEKKSILDYLVKNVEK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=173, Percent_Identity=36.4161849710983, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1790073, Length=158, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17508631, Length=194, Percent_Identity=31.9587628865979, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17554964, Length=193, Percent_Identity=31.0880829015544, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323530, Length=215, Percent_Identity=31.6279069767442, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323927, Length=212, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17933654, Length=188, Percent_Identity=29.2553191489362, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_SULSO (Q9UX09)

Other databases:

- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   G90208
- RefSeq:   NP_342136.1
- ProteinModelPortal:   Q9UX09
- SMR:   Q9UX09
- GeneID:   1454893
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0615
- NMPDR:   fig|273057.1.peg.559
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- PhylomeDB:   Q9UX09
- ProtClustDB:   PRK00455
- BioCyc:   SSOL273057:SSO0615-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 21615; Mature: 21615

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 86-86 BINDING 90-90 BINDING 92-92 BINDING 115-115 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDII
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEE
VGIATGGVPLSAFIACNLKEPMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSI
EEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCEE
EKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHSLFKISEILDELLKSDKLKDN
HHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EKKSILDYLVKNVEK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDII
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEE
VGIATGGVPLSAFIACNLKEPMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSI
EEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCEE
EKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHSLFKISEILDELLKSDKLKDN
HHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EKKSILDYLVKNVEK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726