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The map label for this gene is hisH

Identifier: 15897516

GI number: 15897516

Start: 524987

End: 525586

Strand: Direct

Name: hisH

Synonym: SSO0600

Alternate gene names: 15897516

Gene position: 524987-525586 (Clockwise)

Preceding gene: 15897515

Following gene: 15897517

Centisome position: 17.54

GC content: 34.5

Gene sequence:

>600_bases
ATGAAGGCATTAGTAATTAATTATGGTGTAGGTAATTTATATAGTATCTCATCTGCTCTAAAAAGAGTAGGATTTGAAGT
AACAATAGATAATAAACCAAGGAATGATTACGATCTAATTGTATTTCCTGGTGTCGGAGCATTTTCAGCTGTAGCCGAAT
TTATCTTACGATATAGGGAACTTTTCAATGATCTAAGAAGGAGTGGGACTAATTTTTTAGGAGTTTGTCTAGGCATGCAG
ATAATGTTTGAAAAGGGAACCGAGGGAAAAGAGAGTAATGGCTTAGGATGGTTTAAGGGTATAGTGGATAAGATAAATGC
TAATGTGAAACTTCCTCACATAGGCTGGGATTTAGTATTTGAAGTGAAAGATTCTTGTGAATTAACTTACGGATTAGACA
AGAAATATGTTTACTATGTACATAGCTATGTAGCTTATCCTACTAGTGGAGATTATGTTTACATGAAAAGTCAGTATGGG
ATAGAATATCCTGCATTGGTATGTGATAAGAACGTTGTAGGAACTCAGTTTCACCCGGAGAAAAGTTCAAATACTGGTAA
AATATTTCTCGAGAATCTTAAGGGGTGGATTAAACGTTAA

Upstream 100 bases:

>100_bases
TAAGTGAGGTCGCTGATCTAATCTATCATTTATTAGTATTAATGGCATTAGAAGGCGTAACACCAGAGGATATTTATAGA
GAGTTAGAGAGGAGGAGAAA

Downstream 100 bases:

>100_bases
AACTGAATGAAATAGAAGCACAGAAGATCGCCTCTTTATTAAATTTTAAACATGAGGAAAATACAGTAATTGCCGTAATT
CAAGACTATAAGAGTAAAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRELFNDLRRSGTNFLGVCLGMQ
IMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVFEVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYG
IEYPALVCDKNVVGTQFHPEKSSNTGKIFLENLKGWIKR

Sequences:

>Translated_199_residues
MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRELFNDLRRSGTNFLGVCLGMQ
IMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVFEVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYG
IEYPALVCDKNVVGTQFHPEKSSNTGKIFLENLKGWIKR
>Mature_199_residues
MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRELFNDLRRSGTNFLGVCLGMQ
IMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVFEVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYG
IEYPALVCDKNVVGTQFHPEKSSNTGKIFLENLKGWIKR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=34.3283582089552, Blast_Score=103, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=207, Percent_Identity=29.951690821256, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_SULSO (O33777)

Other databases:

- EMBL:   U82227
- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   H90206
- RefSeq:   NP_342121.1
- ProteinModelPortal:   O33777
- SMR:   O33777
- GeneID:   1454878
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0600
- NMPDR:   fig|273057.1.peg.544
- HOGENOM:   HBG292341
- OMA:   GAYDQGM
- ProtClustDB:   PRK13143
- BioCyc:   SSOL273057:SSO0600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22533; Mature: 22533

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 76-76 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRE
CCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LFNDLRRSGTNFLGVCLGMQIMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVF
HHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEE
EVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYGIEYPALVCDKNVVGTQFHPE
EECCCCEEEECCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCCEECCC
KSSNTGKIFLENLKGWIKR
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRE
CCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LFNDLRRSGTNFLGVCLGMQIMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVF
HHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEE
EVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYGIEYPALVCDKNVVGTQFHPE
EECCCCEEEECCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCCEECCC
KSSNTGKIFLENLKGWIKR
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9209067; 10701121; 11427726