Click here to switch to the map view.

The map label for this gene is recJ [H]

Identifier: 15892684

GI number: 15892684

Start: 725054

End: 726817

Strand: Reverse

Name: recJ [H]

Synonym: RC0761

Alternate gene names: 15892684

Gene position: 726817-725054 (Counterclockwise)

Preceding gene: 15892685

Following gene: 15892683

Centisome position: 57.29

GC content: 33.16

Gene sequence:

>1764_bases
ATGAAACCACAAGCATCTATAAACGGTAAAATCTGGCAGCAGAAACTGATTAACGAAGATATAGTCACCGAGTTATGTCG
TAGTTTCAAAATTAGTGATTTACTTGCTAGGATAGTATCATTAAGAGTAAATAATCTAGAGGAAGTAGAAAATTTCTTGA
GACCGAAAATCAAAAATTTATTACCTGATCCTTTTCATCTACTTGATATGGAAAAGGCTGTAGATAGAACTGTTACAGCT
ATTTTAAATAACCAAAAAATATGTATCTTTGCCGATTATGATGTAGACGGTGCTACTTCAGCCGCTTTACTTAAAAATAT
TTTTAGAGATTTAAATATACTATGTGATATTTATGTTCCTGATCGTATAGCTGAAGGATACGGTCCAACTCCTTTTGCTA
TGCAAAAAATTAAAGATAACGGTACAGAATTATTAATTACGGTTGATTGCGGTGCTATGGCACATGAAGCCCTAAAATAC
GCAAAAGACGTTAACCTTGATGTTATAGTAATCGATCATCATATAGCTACGGAAATATTACCGGATGCAGTAGCTATAGT
AAATCCAAATCGTATTGATGAAAAAAGCGAATATAAACATTTGGCAGCCGTAGGAGTTGCTTTCTTATTTGCAACTGCAA
TATTATCGAATTTAAAGAAGCAAAATTATTTCACGCAAACAATGCTTCAGCCTAATCTAATGCAGTATCTAGATTTAGTG
GCTCTTGGTACTGTTTGCGATATGATGAAATTAACAGGCTTAAATCGTGCATTTGTTGCAAGCGGATTGAAAGTAATGCA
ACAAAGGCACAATATAGGGATTAAAACATTATATGATATGGCAGGGCTTCATGAAATACCAAAATGTTATCATTTAGGGT
TTATTCTAGGTCCGCGTATCAATGCAGGCGGTAGAGTAGGAAAGTCAAGTTTAGGTGCTCATCTCCTATCTACTAGTTGT
TCTAATGAAGCAAATAAATTAGCTAAAGAACTTGAAAAACACAATAATGAGCGTAAAGTAATTGAGTTATTAATGATAGA
AGAAGCGATGGAAATTGCTTTGACACAACAAGATAGTAGTTTATTATTTATCGTAAAAGAAGGATGGCATCCAGGCGTTA
TAGGAATCGTTGCCGGCAAATTAAAAGAAAAATTTGATAAGCCGGTGGCAGTTATAGCTTTAAATGACGGGATTGGTAAA
GCTTCATGCCGTTCTATTTTAGGTATTGATTTTGGTGCTGAAATTATCAATGCTAAAAGCAAAGATTTGCTAATAGCAGG
CGGAGGTCATGCTATGGCGGCAGGTTTTACCGTAACGGCTGAAAAATTACAAGAATTGCAGAATTTTTTTAATAATGCTT
TTAGAATTAGTATAACTAAACTAGAAAATTATAAGCATGCGGAATATGATATTGATTTAACGGTTAATAGTGTGAATTTC
CCTTTAATGGAGGAATTAAACACTTTAGAGCCTTTCGGTCAAGGAAATCATGAGCCTATATTTAAATTTGATGATTTATT
TGTATTAAAAGCGGATATCGTTGGAAGTAAGCATATTAAATGTATGCTTGTTCCAAATAAGCGAGGCTTTGGTAATAAAG
CCTTATCTGCTATTGCTTTTAATTCGGTAGGAACTGCATTTGAAGATGTTTTATTAAGCCCTAAAGCAAAAAATATTGCC
GTAATAGGAACGTTAAAAACTAATAATTGGCAAGATAATTATACTATTCAATTAATTATAAAAGATATACTTCTAAAAGA
GTAG

Upstream 100 bases:

>100_bases
CCCGTGGCTTGACCACGGGGTGAACAGAGGTGAAATTGATCCACGCAACAATGCCTACTCACAATGACGATATACAGCAA
AAATACAGCCTAATAAACAA

Downstream 100 bases:

>100_bases
ATAGACTAAGTATATATAATAACAACTTAAAAAATAATAATTTATGAGAAATGTAAAGAAAACAAAAAATACTACAATTT
CTAAAGACTTAGCAGAAGTA

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPDPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRHNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEANKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
VIGTLKTNNWQDNYTIQLIIKDILLKE

Sequences:

>Translated_587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPDPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRHNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEANKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
VIGTLKTNNWQDNYTIQLIIKDILLKE
>Mature_587_residues
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNLLPDPFHLLDMEKAVDRTVTA
ILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVPDRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKY
AKDVNLDVIVIDHHIATEILPDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
ALGTVCDMMKLTGLNRAFVASGLKVMQQRHNIGIKTLYDMAGLHEIPKCYHLGFILGPRINAGGRVGKSSLGAHLLSTSC
SNEANKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSSLLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGK
ASCRSILGIDFGAEIINAKSKDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAFNSVGTAFEDVLLSPKAKNIA
VIGTLKTNNWQDNYTIQLIIKDILLKE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=570, Percent_Identity=33.6842105263158, Blast_Score=257, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.-

Molecular weight: Translated: 65011; Mature: 65011

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPDPFHLLDMEKAVDRTVTAILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVP
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECC
DRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKYAKDVNLDVIVIDHHIATEIL
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHC
PDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGTVCDMMKLTGLNRAFVASGLKVMQQRHNIGIKTLYDMAGLHEIPKCYHLGFILGPRI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCEEECCCC
NAGGRVGKSSLGAHLLSTSCSNEANKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCC
LLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGKASCRSILGIDFGAEIINAKS
EEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCCC
KDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
CCEEEECCCCEEECCEEEEHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEEEECCCC
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAF
CHHHHHHHCCCCCCCCCCCCEEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHH
NSVGTAFEDVLLSPKAKNIAVIGTLKTNNWQDNYTIQLIIKDILLKE
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MKPQASINGKIWQQKLINEDIVTELCRSFKISDLLARIVSLRVNNLEEVENFLRPKIKNL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPDPFHLLDMEKAVDRTVTAILNNQKICIFADYDVDGATSAALLKNIFRDLNILCDIYVP
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECC
DRIAEGYGPTPFAMQKIKDNGTELLITVDCGAMAHEALKYAKDVNLDVIVIDHHIATEIL
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHC
PDAVAIVNPNRIDEKSEYKHLAAVGVAFLFATAILSNLKKQNYFTQTMLQPNLMQYLDLV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGTVCDMMKLTGLNRAFVASGLKVMQQRHNIGIKTLYDMAGLHEIPKCYHLGFILGPRI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCEEECCCC
NAGGRVGKSSLGAHLLSTSCSNEANKLAKELEKHNNERKVIELLMIEEAMEIALTQQDSS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCC
LLFIVKEGWHPGVIGIVAGKLKEKFDKPVAVIALNDGIGKASCRSILGIDFGAEIINAKS
EEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCCC
KDLLIAGGGHAMAAGFTVTAEKLQELQNFFNNAFRISITKLENYKHAEYDIDLTVNSVNF
CCEEEECCCCEEECCEEEEHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEEEECCCC
PLMEELNTLEPFGQGNHEPIFKFDDLFVLKADIVGSKHIKCMLVPNKRGFGNKALSAIAF
CHHHHHHHCCCCCCCCCCCCEEECCEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHH
NSVGTAFEDVLLSPKAKNIAVIGTLKTNNWQDNYTIQLIIKDILLKE
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]