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The map label for this gene is nuoB [H]

Identifier: 15892407

GI number: 15892407

Start: 479792

End: 480316

Strand: Reverse

Name: nuoB [H]

Synonym: RC0484

Alternate gene names: 15892407

Gene position: 480316-479792 (Counterclockwise)

Preceding gene: 15892408

Following gene: 15892406

Centisome position: 37.86

GC content: 42.29

Gene sequence:

>525_bases
ATGAAAAATAATTTTTATCAAGAAGACGAATTATTAAGTAACGAGCTTTCTAATAGAGGATTCTTACTCACTAAAGTTGA
TGACGTTATAGGCTGGGCGAGAGCTAATTCCTTATGGCCTATGACTTTTGGGCTTGCTTGTTGTGCTGTTGAGATGATGC
AGGCAGCAGCAAGTAGATATGATATGGATCGCTTCGGTATGTTATTTAGACCAAGTCCAAGACAGTCCGACCTTATGATA
GTTGCAGGAACGCTTACTAACAAAATGGCACCAGCTCTACGTAAAGTATATGATCAGATGGCTGAACCTAAATGGGTGCT
TTCAATGGGTAGCTGTGCCAATGGCGGCGGCTATTATCACTTTTCCTATTCGGTAGTGCGTGGCTGTGATAGAATCGTAC
CGGTTGACGTATATGTCCCTGGCTGCCCTCCTACCGCCGAAGCATTAATTTACGGGCTGATGCAGTTACAGAAAAAAATT
AAACGCACTACAGGTTTTAAGTATGACGCTAGACAAACTCATTGA

Upstream 100 bases:

>100_bases
ATAGGTAAAATAGGCTTTTTCTCAATGATGTTTTTCTTATTCGTACTTATTATCGGATTCATATATGAATGGAAGAAAGG
AGCTTTAGACTGGTAGTAAT

Downstream 100 bases:

>100_bases
AAAACTTGCAGCTAAATCTAGCATTTTAATAACTCCGATCACGGTTAAAGATTACCTAGCTTATAAAATCGAGCCGAATT
TTTTATTGCCGTTTTTAAAA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKNNFYQEDELLSNELSNRGFLLTKVDDVIGWARANSLWPMTFGLACCAVEMMQAAASRYDMDRFGMLFRPSPRQSDLMI
VAGTLTNKMAPALRKVYDQMAEPKWVLSMGSCANGGGYYHFSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGLMQLQKKI
KRTTGFKYDARQTH

Sequences:

>Translated_174_residues
MKNNFYQEDELLSNELSNRGFLLTKVDDVIGWARANSLWPMTFGLACCAVEMMQAAASRYDMDRFGMLFRPSPRQSDLMI
VAGTLTNKMAPALRKVYDQMAEPKWVLSMGSCANGGGYYHFSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGLMQLQKKI
KRTTGFKYDARQTH
>Mature_174_residues
MKNNFYQEDELLSNELSNRGFLLTKVDDVIGWARANSLWPMTFGLACCAVEMMQAAASRYDMDRFGMLFRPSPRQSDLMI
VAGTLTNKMAPALRKVYDQMAEPKWVLSMGSCANGGGYYHFSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGLMQLQKKI
KRTTGFKYDARQTH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=79.5918367346939, Blast_Score=261, Evalue=3e-70,
Organism=Escherichia coli, GI1788624, Length=160, Percent_Identity=50.625, Blast_Score=177, Evalue=2e-46,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=42.6229508196721, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=42.2764227642276, Blast_Score=97, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=149, Percent_Identity=71.1409395973154, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=77.8571428571429, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=77.8571428571429, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24651058, Length=149, Percent_Identity=73.1543624161074, Blast_Score=248, Evalue=1e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19616; Mature: 19616

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNFYQEDELLSNELSNRGFLLTKVDDVIGWARANSLWPMTFGLACCAVEMMQAAASRY
CCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DMDRFGMLFRPSPRQSDLMIVAGTLTNKMAPALRKVYDQMAEPKWVLSMGSCANGGGYYH
CHHHCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
FSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGLMQLQKKIKRTTGFKYDARQTH
EHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKNNFYQEDELLSNELSNRGFLLTKVDDVIGWARANSLWPMTFGLACCAVEMMQAAASRY
CCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DMDRFGMLFRPSPRQSDLMIVAGTLTNKMAPALRKVYDQMAEPKWVLSMGSCANGGGYYH
CHHHCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
FSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGLMQLQKKIKRTTGFKYDARQTH
EHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA