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The map label for this gene is def3 [H]

Identifier: 15892003

GI number: 15892003

Start: 72659

End: 73333

Strand: Reverse

Name: def3 [H]

Synonym: RC0080

Alternate gene names: 15892003

Gene position: 73333-72659 (Counterclockwise)

Preceding gene: 15892016

Following gene: 15892000

Centisome position: 5.78

GC content: 30.37

Gene sequence:

>675_bases
ATGTCCAAATTAAAAACAGCTCTATTATTAACTGCTATAATTTTTATAACAGGTTGTAAAAGTAATATGAAAAATGAAAA
CAATATTTCATTACCGCAATATGTAACTTTGAACATTCAAACCTTAAATAGTTCAGAGGATAAAACTATTAGAATAAAGG
CAAAAACTTTAAACTTTCCGTTATCTTCAGAAGATTTACGTGATATATCTATTTTAGAAAAGAAATATGATCAAGAAGAA
AATTGTGCAGGACTTGCTGCACCGCAAATAGGCATATCAAAATGTATAATAATATTTGCAGTACATGAAGATGCAGAGTT
AAAAAAATGGCATCCTGACCTTAAGGATACAATGCCTAAAACTATTTGGATTAACCCCTCATACAAACCGATAGGTATTG
ATAAACATGAAGATTACGAGGGATGCTTTTCTGTTGAAAATGCTACAGGACCGGTAGCAAGATTCAAGAAAATTCATTAT
CACGCATATGATATAAATGGCAATCAAATTCAAGGTATCGCAGAAGGATTTTTAGCAAGAGTTATCCAGCATGAAATCGA
TCATCTAAACGGAAAAGTATTTTTAGATTATGTAGCTCCCAAAAAAATAATGACTAAAGAAGAATATTTAGAAATGCGTA
AAAAAGCAATGGAGCAGGAAAATATAAAATCTTAG

Upstream 100 bases:

>100_bases
TTATTAACTATAGGATGCGTAAAAGAAATATGACAATTTATTTACTTGATTTTAGTTTCTATCTATAATTTTCTATTTAT
AAATTATTATAATAAAATAT

Downstream 100 bases:

>100_bases
AGTTCTCTAAATCTCAAATATCATTCTAGCTAAAATTTGAGTATGTCATTCCCGCGAAAATTTACTTTATATTATTTGGT
ATAATACTATTGAATCGGAT

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSKLKTALLLTAIIFITGCKSNMKNENNISLPQYVTLNIQTLNSSEDKTIRIKAKTLNFPLSSEDLRDISILEKKYDQEE
NCAGLAAPQIGISKCIIIFAVHEDAELKKWHPDLKDTMPKTIWINPSYKPIGIDKHEDYEGCFSVENATGPVARFKKIHY
HAYDINGNQIQGIAEGFLARVIQHEIDHLNGKVFLDYVAPKKIMTKEEYLEMRKKAMEQENIKS

Sequences:

>Translated_224_residues
MSKLKTALLLTAIIFITGCKSNMKNENNISLPQYVTLNIQTLNSSEDKTIRIKAKTLNFPLSSEDLRDISILEKKYDQEE
NCAGLAAPQIGISKCIIIFAVHEDAELKKWHPDLKDTMPKTIWINPSYKPIGIDKHEDYEGCFSVENATGPVARFKKIHY
HAYDINGNQIQGIAEGFLARVIQHEIDHLNGKVFLDYVAPKKIMTKEEYLEMRKKAMEQENIKS
>Mature_223_residues
SKLKTALLLTAIIFITGCKSNMKNENNISLPQYVTLNIQTLNSSEDKTIRIKAKTLNFPLSSEDLRDISILEKKYDQEEN
CAGLAAPQIGISKCIIIFAVHEDAELKKWHPDLKDTMPKTIWINPSYKPIGIDKHEDYEGCFSVENATGPVARFKKIHYH
AYDINGNQIQGIAEGFLARVIQHEIDHLNGKVFLDYVAPKKIMTKEEYLEMRKKAMEQENIKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789682, Length=125, Percent_Identity=36.8, Blast_Score=71, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24645726, Length=138, Percent_Identity=34.7826086956522, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24645728, Length=121, Percent_Identity=32.2314049586777, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 25540; Mature: 25409

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKTALLLTAIIFITGCKSNMKNENNISLPQYVTLNIQTLNSSEDKTIRIKAKTLNFP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEECCC
LSSEDLRDISILEKKYDQEENCAGLAAPQIGISKCIIIFAVHEDAELKKWHPDLKDTMPK
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHCCCCHHHCCCC
TIWINPSYKPIGIDKHEDYEGCFSVENATGPVARFKKIHYHAYDINGNQIQGIAEGFLAR
EEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
VIQHEIDHLNGKVFLDYVAPKKIMTKEEYLEMRKKAMEQENIKS
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKLKTALLLTAIIFITGCKSNMKNENNISLPQYVTLNIQTLNSSEDKTIRIKAKTLNFP
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEECCC
LSSEDLRDISILEKKYDQEENCAGLAAPQIGISKCIIIFAVHEDAELKKWHPDLKDTMPK
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHCCCCHHHCCCC
TIWINPSYKPIGIDKHEDYEGCFSVENATGPVARFKKIHYHAYDINGNQIQGIAEGFLAR
EEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
VIQHEIDHLNGKVFLDYVAPKKIMTKEEYLEMRKKAMEQENIKS
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]