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The map label for this gene is mdh

Identifier: 158425640

GI number: 158425640

Start: 4583947

End: 4584912

Strand: Reverse

Name: mdh

Synonym: AZC_4016

Alternate gene names: 158425640

Gene position: 4584912-4583947 (Counterclockwise)

Preceding gene: 158425641

Following gene: 158425639

Centisome position: 85.38

GC content: 68.53

Gene sequence:

>966_bases
ATGGCGCGCAGCAAGATTGCCCTGATCGGCGCCGGCCAGATCGGCGGCACGCTTGCATTGCTGGCAGGCCTGAAGGAACT
CGGGGACGTCGTGCTGTTCGACCTGGTGGAAGGCGTTCCCCAGGGCAAGGCGCTCGACCTCGCGGAGCTGAGCCCCGTGG
CCGGCTTCGACGCCGCCTTCGCCGGCACCGGCGCCTATGAGCAGATCGCGGGCGCGGATGTGGTGATCGTCACCGCCGGC
GTGCCGCGCAAGCCCGGCATGAGCCGCGACGATCTGCTGGCGGTCAATCTCAAGGTCATGGAGCAGGCCGGCGCGGGCAT
CGCGAAATATGCGCCCGATGCTTTCGTGATCTGCGTCACCAATCCGCTCGACGCCATGGTGTGGGCGCTCCAGAAGGCCT
CCGGCCTGCCGCGTCACAAGGTGGTCGGCATGGCCGGCGTGCTCGACAGCGCGCGGCTGCGCTATTTCCTGGCGGACGAG
TTCAACGTCTCGGTGGAAGACGTGACCGCGATGGTGCTCGGCGGGCACGGCGACACGATGGTGCCGCTGATGCGCTATTG
CACCGTCGCGGGCATCCCCGTTCCCGACCTCATCCGCATCGGCTGGACCAGCACGGACCGCATCGAGGCCATCGTCACGC
GCACCCGCAACGGCGGGGCCGAGATCGTCGAACTGCTCAAGAGCGGCTCGGCCTTCTATGCGCCGGCGGCCTCGGCCATC
GTCATGGCGGAGAGCTATCTCAAGGACAAGAAGCGTGTGCTGCCGGTGGCGGCCCCCCTGTCGGGCGAGTACGGCTTCCG
CGACCTTTACGTGGGCGTGCCGGCGGTGATCGGCGCGCGGGGCGTGGAGCGCATCGTCGAGCTCGAACTCGACCGCGCCG
AGCGCGCGCAATTCGAACAATCGGTGGCCTCGGTCCAAGGACTGGTGGACGCCTGCGCCAAGATCGCGCCGGACCTCGTT
CGGTGA

Upstream 100 bases:

>100_bases
AAAGGGGTAGATGCGGCGTCTTGAACCCTTGTGCGGATGGGAGTAGTCAAGCCCCGCTCGGCCTAAGGCCGTATCCTCTG
TTGCCCGAGGTTTCCTGTTC

Downstream 100 bases:

>100_bases
CACTGCCATCCGCGGCGGCCCTCCGGGTCGCCGTTTTCGTGCCGGCGGGGGGACCCGGCGGCAATAGTCCAACCGGAGCG
GATCGCCTTCGTCATGAGGG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MARSKIALIGAGQIGGTLALLAGLKELGDVVLFDLVEGVPQGKALDLAELSPVAGFDAAFAGTGAYEQIAGADVVIVTAG
VPRKPGMSRDDLLAVNLKVMEQAGAGIAKYAPDAFVICVTNPLDAMVWALQKASGLPRHKVVGMAGVLDSARLRYFLADE
FNVSVEDVTAMVLGGHGDTMVPLMRYCTVAGIPVPDLIRIGWTSTDRIEAIVTRTRNGGAEIVELLKSGSAFYAPAASAI
VMAESYLKDKKRVLPVAAPLSGEYGFRDLYVGVPAVIGARGVERIVELELDRAERAQFEQSVASVQGLVDACAKIAPDLV
R

Sequences:

>Translated_321_residues
MARSKIALIGAGQIGGTLALLAGLKELGDVVLFDLVEGVPQGKALDLAELSPVAGFDAAFAGTGAYEQIAGADVVIVTAG
VPRKPGMSRDDLLAVNLKVMEQAGAGIAKYAPDAFVICVTNPLDAMVWALQKASGLPRHKVVGMAGVLDSARLRYFLADE
FNVSVEDVTAMVLGGHGDTMVPLMRYCTVAGIPVPDLIRIGWTSTDRIEAIVTRTRNGGAEIVELLKSGSAFYAPAASAI
VMAESYLKDKKRVLPVAAPLSGEYGFRDLYVGVPAVIGARGVERIVELELDRAERAQFEQSVASVQGLVDACAKIAPDLV
R
>Mature_320_residues
ARSKIALIGAGQIGGTLALLAGLKELGDVVLFDLVEGVPQGKALDLAELSPVAGFDAAFAGTGAYEQIAGADVVIVTAGV
PRKPGMSRDDLLAVNLKVMEQAGAGIAKYAPDAFVICVTNPLDAMVWALQKASGLPRHKVVGMAGVLDSARLRYFLADEF
NVSVEDVTAMVLGGHGDTMVPLMRYCTVAGIPVPDLIRIGWTSTDRIEAIVTRTRNGGAEIVELLKSGSAFYAPAASAIV
MAESYLKDKKRVLPVAAPLSGEYGFRDLYVGVPAVIGARGVERIVELELDRAERAQFEQSVASVQGLVDACAKIAPDLVR

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=31.0679611650485, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=31.0679611650485, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=31.023102310231, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=31.023102310231, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=29.3548387096774, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI15082234, Length=310, Percent_Identity=28.3870967741935, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=29.3548387096774, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=30.7692307692308, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=30.7692307692308, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=32, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=32, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21735621, Length=315, Percent_Identity=27.9365079365079, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=29.5336787564767, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI103472011, Length=304, Percent_Identity=21.7105263157895, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789632, Length=228, Percent_Identity=34.2105263157895, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=280, Percent_Identity=27.8571428571429, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=323, Percent_Identity=29.7213622291022, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=297, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=30.3149606299213, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=28.2392026578073, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45550422, Length=314, Percent_Identity=26.4331210191083, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24647881, Length=317, Percent_Identity=30.5993690851735, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24663599, Length=304, Percent_Identity=28.2894736842105, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24663595, Length=202, Percent_Identity=32.6732673267327, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_AZOC5 (A8ILC6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001526932.1
- ProteinModelPortal:   A8ILC6
- SMR:   A8ILC6
- GeneID:   5687996
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_4016
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- ProtClustDB:   PRK06223
- BioCyc:   ACAU438753:AZC_4016-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33678; Mature: 33546

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKIALIGAGQIGGTLALLAGLKELGDVVLFDLVEGVPQGKALDLAELSPVAGFDAAF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHH
AGTGAYEQIAGADVVIVTAGVPRKPGMSRDDLLAVNLKVMEQAGAGIAKYAPDAFVICVT
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCHHHCCCCEEEEEEC
NPLDAMVWALQKASGLPRHKVVGMAGVLDSARLRYFLADEFNVSVEDVTAMVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHEEEEEECCCCCHH
VPLMRYCTVAGIPVPDLIRIGWTSTDRIEAIVTRTRNGGAEIVELLKSGSAFYAPAASAI
HHHHHHHHHCCCCCCHHEEECCCCCHHHHHHHEECCCCHHHHHHHHHCCCEEECCHHHHH
VMAESYLKDKKRVLPVAAPLSGEYGFRDLYVGVPAVIGARGVERIVELELDRAERAQFEQ
HHHHHHHHHHHHEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
SVASVQGLVDACAKIAPDLVR
HHHHHHHHHHHHHHHCHHHCC
>Mature Secondary Structure 
ARSKIALIGAGQIGGTLALLAGLKELGDVVLFDLVEGVPQGKALDLAELSPVAGFDAAF
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHH
AGTGAYEQIAGADVVIVTAGVPRKPGMSRDDLLAVNLKVMEQAGAGIAKYAPDAFVICVT
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCHHHCCCCEEEEEEC
NPLDAMVWALQKASGLPRHKVVGMAGVLDSARLRYFLADEFNVSVEDVTAMVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHEEEEEECCCCCHH
VPLMRYCTVAGIPVPDLIRIGWTSTDRIEAIVTRTRNGGAEIVELLKSGSAFYAPAASAI
HHHHHHHHHCCCCCCHHEEECCCCCHHHHHHHEECCCCHHHHHHHHHCCCEEECCHHHHH
VMAESYLKDKKRVLPVAAPLSGEYGFRDLYVGVPAVIGARGVERIVELELDRAERAQFEQ
HHHHHHHHHHHHEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
SVASVQGLVDACAKIAPDLVR
HHHHHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA