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The map label for this gene is lipB [H]

Identifier: 158424463

GI number: 158424463

Start: 3233173

End: 3233916

Strand: Direct

Name: lipB [H]

Synonym: AZC_2839

Alternate gene names: 158424463

Gene position: 3233173-3233916 (Clockwise)

Preceding gene: 158424461

Following gene: 158424465

Centisome position: 60.21

GC content: 70.7

Gene sequence:

>744_bases
GTGGATGGCACCTCCGCCACTCTCCAGAACCTGAGCCAGATGAACGTTTCCTCGCGCCCGCGCTCGACGCTGACCACCGC
CTTCCTGCCGAAGGACGCCGCCGCCCCGCCCGTGGACTGGACCATCAGCGACACGCCCGTCGCCTATCCGGACGCGGTCG
CCGCCATGGAGGCGCGCGCCGCCGCCATTGCCGCGGGCGAGGCGCCGGAACTGGTGTGGCTGGTGGAGCATCCGCCCCTC
TACACCGCCGGAACCAGCGCCCGGCGCGAAGACCTGATCGACCAGCGCTTCCCCGTCTATGAGAGCGGGCGCGGCGGGCA
GTTCACCTATCACGGTCCCGGCCAGCGGGTGACCTATGTGATGCTGGACCTCAAGCGCCGCGATCCGGATCTGCGCCGCT
ATGTGGGGGCGCTGGAGGCGTGGCTGATCGCGACGCTCGCCGCCTTCAACATCACCGGCGAGCGGCGCGAGGACCGGGTG
GGCGTGTGGGTGCCGCGCGGCGGCGGCGTGGAGGACAAGATCGCCGCCATCGGCATCCGCGTGCGCCAGTGGGTGAGCTT
CCACGGCATCTCGCTGAACGTGGACCCGGACCTCACCCATTTCGGCGGCATCGTGCCCTGCGGCGTGCGCGAGCACGGGG
TGACGAGCTTCACCGACCTCGGGCTGCTGGTCTCCATGCCGGAAGTGGACAGCGTTCTGCGCGTCGCCTTCGAGGAGATC
TTCGGGCCGACGCGGCTCGCCTGA

Upstream 100 bases:

>100_bases
TCATTCGAAGCACCCCGGCGCCGGGAGCCCTCGTCTAGCAAATCGAATCCGCGGCCACAAGGCCTCACAGTCGGCTTAAG
ACAGTTCTATATACGCACCT

Downstream 100 bases:

>100_bases
CCGCGCTGGCGCACGGGCGGCGGCAAAACGCGCCGCCCCCTTTCAGAACCGCCGGCCGTTCAGGAAGTCGATGGCCCCTT
GCAGGATGAAGGCGGCGGCG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MDGTSATLQNLSQMNVSSRPRSTLTTAFLPKDAAAPPVDWTISDTPVAYPDAVAAMEARAAAIAAGEAPELVWLVEHPPL
YTAGTSARREDLIDQRFPVYESGRGGQFTYHGPGQRVTYVMLDLKRRDPDLRRYVGALEAWLIATLAAFNITGERREDRV
GVWVPRGGGVEDKIAAIGIRVRQWVSFHGISLNVDPDLTHFGGIVPCGVREHGVTSFTDLGLLVSMPEVDSVLRVAFEEI
FGPTRLA

Sequences:

>Translated_247_residues
MDGTSATLQNLSQMNVSSRPRSTLTTAFLPKDAAAPPVDWTISDTPVAYPDAVAAMEARAAAIAAGEAPELVWLVEHPPL
YTAGTSARREDLIDQRFPVYESGRGGQFTYHGPGQRVTYVMLDLKRRDPDLRRYVGALEAWLIATLAAFNITGERREDRV
GVWVPRGGGVEDKIAAIGIRVRQWVSFHGISLNVDPDLTHFGGIVPCGVREHGVTSFTDLGLLVSMPEVDSVLRVAFEEI
FGPTRLA
>Mature_247_residues
MDGTSATLQNLSQMNVSSRPRSTLTTAFLPKDAAAPPVDWTISDTPVAYPDAVAAMEARAAAIAAGEAPELVWLVEHPPL
YTAGTSARREDLIDQRFPVYESGRGGQFTYHGPGQRVTYVMLDLKRRDPDLRRYVGALEAWLIATLAAFNITGERREDRV
GVWVPRGGGVEDKIAAIGIRVRQWVSFHGISLNVDPDLTHFGGIVPCGVREHGVTSFTDLGLLVSMPEVDSVLRVAFEEI
FGPTRLA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=180, Percent_Identity=37.2222222222222, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI87081767, Length=138, Percent_Identity=47.1014492753623, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355483, Length=175, Percent_Identity=35.4285714285714, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26913; Mature: 26913

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTSATLQNLSQMNVSSRPRSTLTTAFLPKDAAAPPVDWTISDTPVAYPDAVAAMEARA
CCCHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
AAIAAGEAPELVWLVEHPPLYTAGTSARREDLIDQRFPVYESGRGGQFTYHGPGQRVTYV
HHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEEE
MLDLKRRDPDLRRYVGALEAWLIATLAAFNITGERREDRVGVWVPRGGGVEDKIAAIGIR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VRQWVSFHGISLNVDPDLTHFGGIVPCGVREHGVTSFTDLGLLVSMPEVDSVLRVAFEEI
HHHHHHHCCEEEECCCCHHHHCCEEECCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHH
FGPTRLA
CCCCCCC
>Mature Secondary Structure
MDGTSATLQNLSQMNVSSRPRSTLTTAFLPKDAAAPPVDWTISDTPVAYPDAVAAMEARA
CCCHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
AAIAAGEAPELVWLVEHPPLYTAGTSARREDLIDQRFPVYESGRGGQFTYHGPGQRVTYV
HHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEEE
MLDLKRRDPDLRRYVGALEAWLIATLAAFNITGERREDRVGVWVPRGGGVEDKIAAIGIR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VRQWVSFHGISLNVDPDLTHFGGIVPCGVREHGVTSFTDLGLLVSMPEVDSVLRVAFEEI
HHHHHHHCCEEEECCCCHHHHCCEEECCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHH
FGPTRLA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]