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The map label for this gene is nodB [H]

Identifier: 158424461

GI number: 158424461

Start: 3230771

End: 3232165

Strand: Direct

Name: nodB [H]

Synonym: AZC_2837

Alternate gene names: 158424461

Gene position: 3230771-3232165 (Clockwise)

Preceding gene: 158424460

Following gene: 158424463

Centisome position: 60.17

GC content: 72.19

Gene sequence:

>1395_bases
TTGTCTCCACCGCCCGCAGGCGCAGAACCTGCGGACGCGGAAGCGCCACGGGCCGTTCGCAACGGGCGGCCCCGTCTTCA
GAAGCGGCGTCGCCGTCCGGCGCCATGCGGGTTACGGCACATGCGTTCCATCCTCCTGGCGGGGCTCGTCGCCTCCATCG
CAAGTTCAGCCCTCGCACAGTCGGTTCTCCCGGCCGCGCCTTCCCCGCAGTTGCCGGCCGCTCCGGCGCTTCCCGCCGCT
GCCCCCATCGCTCCCGCGGTCCCGGCCGCTGCCGCCGCGAGCGCCACGCCCGCCAGCACCGGGGCCGGCCTGACGCCGCC
GCCGCAACTGCCGCCGCCCTCCGCCTGCCCCGGCAATCCCGATGCGCTCGGTGTCTCGCGGGTGATCGCCATCGATCCGA
AGACCAGCCCCTTCCTCGGCCGGCTGCAATACAAGCAGACGCTGCCGCTGGAAAAAGGCGAGGTGGTGCTGACCTTTGAC
GATGGCCCGCTGGCGCCCATGACCACCCGCGTGCTCGACACGCTGGCCCATGAGTGCGTGAAGGCCACCTTCTTCATCGT
CGGCCGCATGGCCCATTCCAATCCGCACCTGCTGCAGCGGACGCTGGCGGACGGCCACACCATCGGCACCCACAGCGAGA
CCCATCCGCTCATCTTCCCCAAGCTCACGCAGGAAGCGGGCGTTCAGGAGATCGAGCAGGGCTTCGCCTCCGTCACCAAG
GCGCTCGCGCCGGTGGGCGCCGCGCCCAATCCGTGGTTCCGCTTCCCCGGTCTCGGCCGCACCAAATTCTACGAGGCCTA
TACGCAGGAGCACGGCATCGCCGTCTTCAGCGTGGACGAGGTGGCCGACGACTGGATGCATCTGAAGCCGGAAGAGGTGG
AGAAGCGTGCCCTCGACCGGCTGGAGGCGCGGGGCGGCGGCATCCTGCTGCTGCACGACATCCAGCCTGCCACTGCCGTC
ATGCTGCCGAACCTGCTGCGCGAACTGAAGGCGCGCGGCTTCCGCATCGTGCATGTGGTGCCGGCGACCGCCGGCATGGT
GGCGAGCCTCCCCGGCCCCGGTCCGCGCGGGCATATCCAGCCCGCCGCAGCCAGCGCGACCACCGCCGCGCCCGGCACCG
CTCCGGCCAAGCCCGCGCAACCCAAGCCCGCGACCGCGCCGAAGCCCGCAACACCCGCGACGGCCACCGCCCGTCCCGTC
CCCCCCACACCGCGGACGACAGCGACCACCGCCGCCGCGCCGGCTGAACCCGCCGCCACGGGCGCCCCCCAGGCCATCTC
CCCCCACGCGCAGGCCGGTGATGGTGGCTCCGATGCGCCGGGCCTCGGCCAGTTCTGGCCGCGCCTGTGGAACGACACGA
TGAAGGGCCTGTCGGGCGGCACCGACAAGAACTGA

Upstream 100 bases:

>100_bases
ACGGCGGCCATGATTCCGGCGTTCTTGCGGGCTTTAGCTCAGCGTGGCTACCGCGTGGTCCACATGGTTCCCGCCCGCCA
GCGCGCGACCGAACGTTAGC

Downstream 100 bases:

>100_bases
GAGGCGTGGTTGCGGTTCTGGCCCGACCGCCGCCCTCCAGTAGACTGGATTGGTCTCAGTCTTCGAAGCGGCGCTGCATG
AGCAGAACGTCGTGCCTCTG

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSPPPAGAEPADAEAPRAVRNGRPRLQKRRRRPAPCGLRHMRSILLAGLVASIASSALAQSVLPAAPSPQLPAAPALPAA
APIAPAVPAAAAASATPASTGAGLTPPPQLPPPSACPGNPDALGVSRVIAIDPKTSPFLGRLQYKQTLPLEKGEVVLTFD
DGPLAPMTTRVLDTLAHECVKATFFIVGRMAHSNPHLLQRTLADGHTIGTHSETHPLIFPKLTQEAGVQEIEQGFASVTK
ALAPVGAAPNPWFRFPGLGRTKFYEAYTQEHGIAVFSVDEVADDWMHLKPEEVEKRALDRLEARGGGILLLHDIQPATAV
MLPNLLRELKARGFRIVHVVPATAGMVASLPGPGPRGHIQPAAASATTAAPGTAPAKPAQPKPATAPKPATPATATARPV
PPTPRTTATTAAAPAEPAATGAPQAISPHAQAGDGGSDAPGLGQFWPRLWNDTMKGLSGGTDKN

Sequences:

>Translated_464_residues
MSPPPAGAEPADAEAPRAVRNGRPRLQKRRRRPAPCGLRHMRSILLAGLVASIASSALAQSVLPAAPSPQLPAAPALPAA
APIAPAVPAAAAASATPASTGAGLTPPPQLPPPSACPGNPDALGVSRVIAIDPKTSPFLGRLQYKQTLPLEKGEVVLTFD
DGPLAPMTTRVLDTLAHECVKATFFIVGRMAHSNPHLLQRTLADGHTIGTHSETHPLIFPKLTQEAGVQEIEQGFASVTK
ALAPVGAAPNPWFRFPGLGRTKFYEAYTQEHGIAVFSVDEVADDWMHLKPEEVEKRALDRLEARGGGILLLHDIQPATAV
MLPNLLRELKARGFRIVHVVPATAGMVASLPGPGPRGHIQPAAASATTAAPGTAPAKPAQPKPATAPKPATPATATARPV
PPTPRTTATTAAAPAEPAATGAPQAISPHAQAGDGGSDAPGLGQFWPRLWNDTMKGLSGGTDKN
>Mature_463_residues
SPPPAGAEPADAEAPRAVRNGRPRLQKRRRRPAPCGLRHMRSILLAGLVASIASSALAQSVLPAAPSPQLPAAPALPAAA
PIAPAVPAAAAASATPASTGAGLTPPPQLPPPSACPGNPDALGVSRVIAIDPKTSPFLGRLQYKQTLPLEKGEVVLTFDD
GPLAPMTTRVLDTLAHECVKATFFIVGRMAHSNPHLLQRTLADGHTIGTHSETHPLIFPKLTQEAGVQEIEQGFASVTKA
LAPVGAAPNPWFRFPGLGRTKFYEAYTQEHGIAVFSVDEVADDWMHLKPEEVEKRALDRLEARGGGILLLHDIQPATAVM
LPNLLRELKARGFRIVHVVPATAGMVASLPGPGPRGHIQPAAASATTAAPGTAPAKPAQPKPATAPKPATPATATARPVP
PTPRTTATTAAAPAEPAATGAPQAISPHAQAGDGGSDAPGLGQFWPRLWNDTMKGLSGGTDKN

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=161, Percent_Identity=32.2981366459627, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 47866; Mature: 47735

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPPAGAEPADAEAPRAVRNGRPRLQKRRRRPAPCGLRHMRSILLAGLVASIASSALAQ
CCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVLPAAPSPQLPAAPALPAAAPIAPAVPAAAAASATPASTGAGLTPPPQLPPPSACPGNP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DALGVSRVIAIDPKTSPFLGRLQYKQTLPLEKGEVVLTFDDGPLAPMTTRVLDTLAHECV
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
KATFFIVGRMAHSNPHLLQRTLADGHTIGTHSETHPLIFPKLTQEAGVQEIEQGFASVTK
HHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHH
ALAPVGAAPNPWFRFPGLGRTKFYEAYTQEHGIAVFSVDEVADDWMHLKPEEVEKRALDR
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
LEARGGGILLLHDIQPATAVMLPNLLRELKARGFRIVHVVPATAGMVASLPGPGPRGHIQ
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCC
PAAASATTAAPGTAPAKPAQPKPATAPKPATPATATARPVPPTPRTTATTAAAPAEPAAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAPQAISPHAQAGDGGSDAPGLGQFWPRLWNDTMKGLSGGTDKN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SPPPAGAEPADAEAPRAVRNGRPRLQKRRRRPAPCGLRHMRSILLAGLVASIASSALAQ
CCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVLPAAPSPQLPAAPALPAAAPIAPAVPAAAAASATPASTGAGLTPPPQLPPPSACPGNP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DALGVSRVIAIDPKTSPFLGRLQYKQTLPLEKGEVVLTFDDGPLAPMTTRVLDTLAHECV
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
KATFFIVGRMAHSNPHLLQRTLADGHTIGTHSETHPLIFPKLTQEAGVQEIEQGFASVTK
HHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCEECHHHHHHHHHHHHHHHHHHHHH
ALAPVGAAPNPWFRFPGLGRTKFYEAYTQEHGIAVFSVDEVADDWMHLKPEEVEKRALDR
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
LEARGGGILLLHDIQPATAVMLPNLLRELKARGFRIVHVVPATAGMVASLPGPGPRGHIQ
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCC
PAAASATTAAPGTAPAKPAQPKPATAPKPATPATATARPVPPTPRTTATTAAAPAEPAAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAPQAISPHAQAGDGGSDAPGLGQFWPRLWNDTMKGLSGGTDKN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968; 8850088 [H]