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The map label for this gene is miaA

Identifier: 158424199

GI number: 158424199

Start: 2901409

End: 2902347

Strand: Direct

Name: miaA

Synonym: AZC_2575

Alternate gene names: 158424199

Gene position: 2901409-2902347 (Clockwise)

Preceding gene: 158424197

Following gene: 158424201

Centisome position: 54.03

GC content: 70.18

Gene sequence:

>939_bases
ATGGGACTGCGGGACTCTGAAGCATTGAAACCATTGGCGGTGCTCATCGCAGGTCCAACGGCCAGCGGCAAGTCGGCGCT
GGCGCTGGATCTGGCGGAACGGACCGACGGCGTCATCATCAACGCCGATTCCATGCAGGTTTATGGCGATCTGCGCATTT
TGACCGCCCGGCCGAGCCCGGAGGAGGAAATGCGGGTGCCCCACCGCCTCTACGGCCACGTGGACGGCGCCGCCGATTAC
TCCGTCGCGCGCTGGATCGCCGATGCAGCGGCGGCGATGGCCACCGCCCGCGCAGAGGGGCGCCTGCCCATCGTCATCGG
CGGCACGGGCCTTTATTTCCGGGCGCTGACGCGGGGCCTTGCTCCCATTCCCGAGATCCCCGAGGAGGTGCGTCAGCGCG
TGCGGCGCATGGCCGAAGACGAGGTGACGGTGGTGCTGCACGCCCGCCTCGCCACCCGTGACCCGGAAGCCGCCGTGCGC
CTGCAGCCGCAGGATCGCCAGCGCATCCTGCGGGCGCTGGAGGTGTTCGAGGCCACCGGCCAGCCGCTCAGCCAGTGGCA
GCGCGCCACCCACCGGCCGGTGCTGGAGGAAGCGTCCGCCGTGCGCTTCGTGCTGGAGGTGGAACGCGAAACGCTGCGGG
GACGCATCGACCGCCGCTTCGAGACCATGATGGAGGCTGGCGCGCTGGCTGAGGTGGAGCGGCTGGCGGCGCGCGAACTG
CCAGCGGACCGGACCATCCTGAAGGCCCATGGCGCCCCTGCCCTCACCCGCTATCTGCGGGGCGAGATGTCGCGGGCGGA
CGCCATCGCGGAAGGCCAGAACGACACCCGCCGCTATGCCAAGCGGCAGGTCACGTGGTTCCGCCACCAGATGCCGGACT
GGATGCGCGGCACGCCCGACACCGCGCTCGATCAACTGACCGGGACGCTTCGCCTCTGA

Upstream 100 bases:

>100_bases
GGGCTCGTCCGCCCCGGGCAGAACCGCGCGGGCGGCAGCCACCACCTCGCGGGTTATGGCGGGGTGGGCGGGATTTGAGA
TAAGTGTCGCGACATAGACC

Downstream 100 bases:

>100_bases
TCAGGCGCGCGCCCGCGCCACCTTCGCGGGCGGAAGCACCTTGAGGCGCACCGTGCCGGCGAACGCGACGCCGAGCCCCG
CTGCCAGCACCCACAGCGCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGLRDSEALKPLAVLIAGPTASGKSALALDLAERTDGVIINADSMQVYGDLRILTARPSPEEEMRVPHRLYGHVDGAADY
SVARWIADAAAAMATARAEGRLPIVIGGTGLYFRALTRGLAPIPEIPEEVRQRVRRMAEDEVTVVLHARLATRDPEAAVR
LQPQDRQRILRALEVFEATGQPLSQWQRATHRPVLEEASAVRFVLEVERETLRGRIDRRFETMMEAGALAEVERLAAREL
PADRTILKAHGAPALTRYLRGEMSRADAIAEGQNDTRRYAKRQVTWFRHQMPDWMRGTPDTALDQLTGTLRL

Sequences:

>Translated_312_residues
MGLRDSEALKPLAVLIAGPTASGKSALALDLAERTDGVIINADSMQVYGDLRILTARPSPEEEMRVPHRLYGHVDGAADY
SVARWIADAAAAMATARAEGRLPIVIGGTGLYFRALTRGLAPIPEIPEEVRQRVRRMAEDEVTVVLHARLATRDPEAAVR
LQPQDRQRILRALEVFEATGQPLSQWQRATHRPVLEEASAVRFVLEVERETLRGRIDRRFETMMEAGALAEVERLAAREL
PADRTILKAHGAPALTRYLRGEMSRADAIAEGQNDTRRYAKRQVTWFRHQMPDWMRGTPDTALDQLTGTLRL
>Mature_311_residues
GLRDSEALKPLAVLIAGPTASGKSALALDLAERTDGVIINADSMQVYGDLRILTARPSPEEEMRVPHRLYGHVDGAADYS
VARWIADAAAAMATARAEGRLPIVIGGTGLYFRALTRGLAPIPEIPEEVRQRVRRMAEDEVTVVLHARLATRDPEAAVRL
QPQDRQRILRALEVFEATGQPLSQWQRATHRPVLEEASAVRFVLEVERETLRGRIDRRFETMMEAGALAEVERLAARELP
ADRTILKAHGAPALTRYLRGEMSRADAIAEGQNDTRRYAKRQVTWFRHQMPDWMRGTPDTALDQLTGTLRL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=324, Percent_Identity=29.6296296296296, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1790613, Length=284, Percent_Identity=39.4366197183099, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=24.4725738396624, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=28.9115646258503, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=33.4586466165414, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_AZOC5 (A8IAX3)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525491.1
- ProteinModelPortal:   A8IAX3
- SMR:   A8IAX3
- GeneID:   5692433
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2575
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   ACAU438753:AZC_2575-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34779; Mature: 34648

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRDSEALKPLAVLIAGPTASGKSALALDLAERTDGVIINADSMQVYGDLRILTARPSP
CCCCCCHHHCCEEEEEECCCCCCCCEEEEEHHHCCCCEEEECCCEEEEEEEEEEEECCCC
EEEMRVPHRLYGHVDGAADYSVARWIADAAAAMATARAEGRLPIVIGGTGLYFRALTRGL
HHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCC
APIPEIPEEVRQRVRRMAEDEVTVVLHARLATRDPEAAVRLQPQDRQRILRALEVFEATG
CCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCC
QPLSQWQRATHRPVLEEASAVRFVLEVERETLRGRIDRRFETMMEAGALAEVERLAAREL
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADRTILKAHGAPALTRYLRGEMSRADAIAEGQNDTRRYAKRQVTWFRHQMPDWMRGTPD
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCH
TALDQLTGTLRL
HHHHHHHHHCCC
>Mature Secondary Structure 
GLRDSEALKPLAVLIAGPTASGKSALALDLAERTDGVIINADSMQVYGDLRILTARPSP
CCCCCHHHCCEEEEEECCCCCCCCEEEEEHHHCCCCEEEECCCEEEEEEEEEEEECCCC
EEEMRVPHRLYGHVDGAADYSVARWIADAAAAMATARAEGRLPIVIGGTGLYFRALTRGL
HHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCC
APIPEIPEEVRQRVRRMAEDEVTVVLHARLATRDPEAAVRLQPQDRQRILRALEVFEATG
CCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCC
QPLSQWQRATHRPVLEEASAVRFVLEVERETLRGRIDRRFETMMEAGALAEVERLAAREL
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADRTILKAHGAPALTRYLRGEMSRADAIAEGQNDTRRYAKRQVTWFRHQMPDWMRGTPD
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCH
TALDQLTGTLRL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA