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The map label for this gene is pleD [H]

Identifier: 158424036

GI number: 158424036

Start: 2739331

End: 2740434

Strand: Direct

Name: pleD [H]

Synonym: AZC_2412

Alternate gene names: 158424036

Gene position: 2739331-2740434 (Clockwise)

Preceding gene: 158424035

Following gene: 158424037

Centisome position: 51.01

GC content: 67.93

Gene sequence:

>1104_bases
GTGCGTGACAGCGCTCTAGCATGCGCTTTGCCTTCTGATTCCGTGGGGCACGGCGGATGCGACAGCGCTCGGCCGCGGAG
GTTCACCTGCGCGCGACCGCGCCGCATGCCCGGCCCTGCATTTTCAGTCGCGCACCTCGTCATAGTGCAGAGGGTGGTGA
GCATGCGAATTGTCCTGGTGGATGCGAGCCGCGTCACGCTCAAGCTCATTGGCCGGATGCTCACGGATAATGGCCACGAG
GTGGTCTGCTTCAGCGACGGCCTTGAGGCGCTGGCCTATCTGCACGGCGGGTCGGAGATCGACCTGCTCATGACCAGCTT
CGAGACACCCGGTGTGCCCGGCATCCAGCTGTGCTGGGAGGCACGGCTGATCGCGAACGGCGATCGCCCGCTCTACGTGA
TCGCCATGTCCTCCAGCAACGACGCGGACCATCTGGCGGAGGCTCTGGACAGCGGCGCGGACGATTTCATCCGCAAGCCC
CCCGCCCCCATGGAACTGGACGCCCGCCTTCGCGCCGCCGAGCGGCTCAATCAGGCGCGGCGCGAACTGGTCCGGCTCGC
GACCCGCGACGGGCTGACCGGACTGCTGAACCGCCGCGCCTTCTTCATCCAGGCGGAAGACCTGCTGCGTCATGGCATCG
TGGCCTCCGTGATCCTGCTCGACATCGACCACTTCAAGCAGGTCAACGACACCTACGGACATGACGGCGGCGACGTGGTC
CTGACCGCCGTGGCACGGGCCATGTCGGCTTATGCCGGCCGGGCGGCGCGCGTTGGGGGCGAGGAGTTCGCCATTCTCCT
GCCAGGTGTCACGGAGAGCGACGCCTACGAGGAGGCAGAACGGCTGCGCTGGCGGATCGCCGACACGGCGGTGACGCTAC
GTGATGGACGAACGATCTCGGTGACCGCCTCGCTCGGCGTCGCCGAGCACGCCACCGACGACAATGCCGACGAATGGATG
AAGCGCGCCGACATTGCGCTCTATGCCGCCAAGACCGGCGGCCGGAACCGTACGGTCATGGGCGAAGAGGTGTCCGACGT
GGACGGGCGGGGCTCGGGCCTTGCCCGGAGCGGCGGCGGACGGGTGCGCCAGCGCGCCGAGTAG

Upstream 100 bases:

>100_bases
CAGGCTGAGCCTGTCCGTGGGACCGCCTTATTGCAAGCGCGGCTGAATGTATCGTTGCACCGCCGCCGGAAACATCCCAT
AGCCCTGTCTGTCATGGATC

Downstream 100 bases:

>100_bases
GGGAGCGGACCGGGAGCGATTCCCCCTTGCCCCGGCAGTCCCGATCCGATAAAGCCGCGCTTTCCTTTGCGGGGACGAAC
GCGATGGGGCAGCGGCCGGT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRDSALACALPSDSVGHGGCDSARPRRFTCARPRRMPGPAFSVAHLVIVQRVVSMRIVLVDASRVTLKLIGRMLTDNGHE
VVCFSDGLEALAYLHGGSEIDLLMTSFETPGVPGIQLCWEARLIANGDRPLYVIAMSSSNDADHLAEALDSGADDFIRKP
PAPMELDARLRAAERLNQARRELVRLATRDGLTGLLNRRAFFIQAEDLLRHGIVASVILLDIDHFKQVNDTYGHDGGDVV
LTAVARAMSAYAGRAARVGGEEFAILLPGVTESDAYEEAERLRWRIADTAVTLRDGRTISVTASLGVAEHATDDNADEWM
KRADIALYAAKTGGRNRTVMGEEVSDVDGRGSGLARSGGGRVRQRAE

Sequences:

>Translated_367_residues
MRDSALACALPSDSVGHGGCDSARPRRFTCARPRRMPGPAFSVAHLVIVQRVVSMRIVLVDASRVTLKLIGRMLTDNGHE
VVCFSDGLEALAYLHGGSEIDLLMTSFETPGVPGIQLCWEARLIANGDRPLYVIAMSSSNDADHLAEALDSGADDFIRKP
PAPMELDARLRAAERLNQARRELVRLATRDGLTGLLNRRAFFIQAEDLLRHGIVASVILLDIDHFKQVNDTYGHDGGDVV
LTAVARAMSAYAGRAARVGGEEFAILLPGVTESDAYEEAERLRWRIADTAVTLRDGRTISVTASLGVAEHATDDNADEWM
KRADIALYAAKTGGRNRTVMGEEVSDVDGRGSGLARSGGGRVRQRAE
>Mature_367_residues
MRDSALACALPSDSVGHGGCDSARPRRFTCARPRRMPGPAFSVAHLVIVQRVVSMRIVLVDASRVTLKLIGRMLTDNGHE
VVCFSDGLEALAYLHGGSEIDLLMTSFETPGVPGIQLCWEARLIANGDRPLYVIAMSSSNDADHLAEALDSGADDFIRKP
PAPMELDARLRAAERLNQARRELVRLATRDGLTGLLNRRAFFIQAEDLLRHGIVASVILLDIDHFKQVNDTYGHDGGDVV
LTAVARAMSAYAGRAARVGGEEFAILLPGVTESDAYEEAERLRWRIADTAVTLRDGRTISVTASLGVAEHATDDNADEWM
KRADIALYAAKTGGRNRTVMGEEVSDVDGRGSGLARSGGGRVRQRAE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=40.6060606060606, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=37.0786516853933, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=41.25, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=35.9116022099448, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=35.8823529411765, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=34.8066298342541, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=38.0681818181818, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=35.1851851851852, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=33.7349397590361, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=35.672514619883, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=33.5294117647059, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=33.1288343558282, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39701; Mature: 39701

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDSALACALPSDSVGHGGCDSARPRRFTCARPRRMPGPAFSVAHLVIVQRVVSMRIVLV
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEE
DASRVTLKLIGRMLTDNGHEVVCFSDGLEALAYLHGGSEIDLLMTSFETPGVPGIQLCWE
ECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHEE
ARLIANGDRPLYVIAMSSSNDADHLAEALDSGADDFIRKPPAPMELDARLRAAERLNQAR
EEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
RELVRLATRDGLTGLLNRRAFFIQAEDLLRHGIVASVILLDIDHFKQVNDTYGHDGGDVV
HHHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LTAVARAMSAYAGRAARVGGEEFAILLPGVTESDAYEEAERLRWRIADTAVTLRDGRTIS
HHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHEEEECEEEEEECCCEEE
VTASLGVAEHATDDNADEWMKRADIALYAAKTGGRNRTVMGEEVSDVDGRGSGLARSGGG
EEEECCCHHCCCCCCHHHHHHHHCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCC
RVRQRAE
CCCCCCC
>Mature Secondary Structure
MRDSALACALPSDSVGHGGCDSARPRRFTCARPRRMPGPAFSVAHLVIVQRVVSMRIVLV
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEE
DASRVTLKLIGRMLTDNGHEVVCFSDGLEALAYLHGGSEIDLLMTSFETPGVPGIQLCWE
ECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHEE
ARLIANGDRPLYVIAMSSSNDADHLAEALDSGADDFIRKPPAPMELDARLRAAERLNQAR
EEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
RELVRLATRDGLTGLLNRRAFFIQAEDLLRHGIVASVILLDIDHFKQVNDTYGHDGGDVV
HHHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LTAVARAMSAYAGRAARVGGEEFAILLPGVTESDAYEEAERLRWRIADTAVTLRDGRTIS
HHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHEEEECEEEEEECCCEEE
VTASLGVAEHATDDNADEWMKRADIALYAAKTGGRNRTVMGEEVSDVDGRGSGLARSGGG
EEEECCCHHCCCCCCHHHHHHHHCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCC
RVRQRAE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA