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The map label for this gene is pleD [H]

Identifier: 158424035

GI number: 158424035

Start: 2737948

End: 2739117

Strand: Direct

Name: pleD [H]

Synonym: AZC_2411

Alternate gene names: 158424035

Gene position: 2737948-2739117 (Clockwise)

Preceding gene: 158424034

Following gene: 158424036

Centisome position: 50.99

GC content: 65.38

Gene sequence:

>1170_bases
ATGCCGCTGTTGCAGACTTGTCCTAGAGTTTCAACTCTGGCGACCTTTTGCCATATTGCGGAAACCTGTGTTGTAACTGT
TTGTCCGGCACATATCCGCCAATATGGAAATATTGCACCCGCCTCGTCGCCAGAATGGCGCCAAATCAAGGGCGGGCTTT
ATGGCGCAGCCCACCCCGGCAGAGTTACTGTTCAGCTAACCACAGTTCTTTGCTTCCATGAGTTGGGCCCCATGCGGATT
ATGTTGGTCGACGCGAGCCGCGTCACGCTGAAGCTCCTCGCCAAGACGCTGCTCGAAAGCCGGCATGACGTCGTTGCCTT
CAGCGATGGCGGCGAGGCCCTCAACTATCTGCAGAGCGGGGCCGAGATCGACCTGCTCATGACCAGCTTCGAAATTCCCG
GCGTGTCCGGCATCCAGCTCTGCTGGGAAGCGCGGCTGATCGCCAGCAGCGGCCGCCCGCTCTACGTGATCGCCATGTCG
TCCAACAACGACGCCGACCATCTGGCCGAAGCGCTGGACAGCGGCGCGGACGACTTCGTCAGCAAGCCGCCGTCCCCGGT
GGAACTCGAGGCCCGTCTGCGCGCCGCCGAGCGCATGAACCTCGCCCAGCGCGAACTGGTCCGCCTCGCCACCCGCGACG
CCATGACCGATCTGCTGAACCGGCGCACCTTCTTCGCCCGCGCCGAGGACATGATCCACAACCGCACCAAGGCCTCCGTC
ATCCTGCTCGACGTGGACCTGCTGCGTCACATCAACGAGACCCACGGCTATCCCGGCGGCGACACCGTGGTGATGAAGGT
GGCGCAGTCGATGAAGCGCTACCAGGGCATGGCCGCGCGCATCGGCGGCGAGGAGTTCGCCGTGCTGCTACCCGGCCTTT
CCGAGGCCGAGGCGGTGGAGGAAGGCGAGCGCCTGCGCTGGTCGATCGCCGATCAGGCCGTCACCATGCCCAATGGGCAA
CTGATCACCGTCACGGCCTCCCTCGGCGTCGCCGAACGCAAGCCCGATGACAATGTGGACGAGTGGATGGAGAGCGCGGA
CATCGCGCTCTATGCCGCGAAGGCCGGTGGGCGCAACCGCGTGGTCATGTCCGAGGAGATCATGGACCCGGACGCGCCCC
GCGCCGGCCTCGCCCGCGGCGGCGGCGGCCGCGCGCTGCGTGGCGGCTGA

Upstream 100 bases:

>100_bases
GCTGCATCTGCGCCTCAACGACGGCACCTATGCGCGGATTTCCGCCACCTGGATTCGGTGAACGAGGCCGGAGGGCGGGG
CGGTTTGCGCCCTGCCCATC

Downstream 100 bases:

>100_bases
GGACGACCCAAGGGCGGCCAGTGGCCGCCCTTCGCGTTTGTGCGGTGTGCCCGCGCTCCGCAGGTCCGGCCCATATGGAC
GGCCCGCCAGTCGCCGCGGG

Product: REC protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPLLQTCPRVSTLATFCHIAETCVVTVCPAHIRQYGNIAPASSPEWRQIKGGLYGAAHPGRVTVQLTTVLCFHELGPMRI
MLVDASRVTLKLLAKTLLESRHDVVAFSDGGEALNYLQSGAEIDLLMTSFEIPGVSGIQLCWEARLIASSGRPLYVIAMS
SNNDADHLAEALDSGADDFVSKPPSPVELEARLRAAERMNLAQRELVRLATRDAMTDLLNRRTFFARAEDMIHNRTKASV
ILLDVDLLRHINETHGYPGGDTVVMKVAQSMKRYQGMAARIGGEEFAVLLPGLSEAEAVEEGERLRWSIADQAVTMPNGQ
LITVTASLGVAERKPDDNVDEWMESADIALYAAKAGGRNRVVMSEEIMDPDAPRAGLARGGGGRALRGG

Sequences:

>Translated_389_residues
MPLLQTCPRVSTLATFCHIAETCVVTVCPAHIRQYGNIAPASSPEWRQIKGGLYGAAHPGRVTVQLTTVLCFHELGPMRI
MLVDASRVTLKLLAKTLLESRHDVVAFSDGGEALNYLQSGAEIDLLMTSFEIPGVSGIQLCWEARLIASSGRPLYVIAMS
SNNDADHLAEALDSGADDFVSKPPSPVELEARLRAAERMNLAQRELVRLATRDAMTDLLNRRTFFARAEDMIHNRTKASV
ILLDVDLLRHINETHGYPGGDTVVMKVAQSMKRYQGMAARIGGEEFAVLLPGLSEAEAVEEGERLRWSIADQAVTMPNGQ
LITVTASLGVAERKPDDNVDEWMESADIALYAAKAGGRNRVVMSEEIMDPDAPRAGLARGGGGRALRGG
>Mature_388_residues
PLLQTCPRVSTLATFCHIAETCVVTVCPAHIRQYGNIAPASSPEWRQIKGGLYGAAHPGRVTVQLTTVLCFHELGPMRIM
LVDASRVTLKLLAKTLLESRHDVVAFSDGGEALNYLQSGAEIDLLMTSFEIPGVSGIQLCWEARLIASSGRPLYVIAMSS
NNDADHLAEALDSGADDFVSKPPSPVELEARLRAAERMNLAQRELVRLATRDAMTDLLNRRTFFARAEDMIHNRTKASVI
LLDVDLLRHINETHGYPGGDTVVMKVAQSMKRYQGMAARIGGEEFAVLLPGLSEAEAVEEGERLRWSIADQAVTMPNGQL
ITVTASLGVAERKPDDNVDEWMESADIALYAAKAGGRNRVVMSEEIMDPDAPRAGLARGGGGRALRGG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=181, Percent_Identity=35.9116022099448, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=36.5269461077844, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=31.8181818181818, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=36.6279069767442, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=32.4022346368715, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=28.6549707602339, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=33.1395348837209, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=183, Percent_Identity=28.9617486338798, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=30.188679245283, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=29.4797687861272, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42186; Mature: 42055

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLQTCPRVSTLATFCHIAETCVVTVCPAHIRQYGNIAPASSPEWRQIKGGLYGAAHPG
CCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCC
RVTVQLTTVLCFHELGPMRIMLVDASRVTLKLLAKTLLESRHDVVAFSDGGEALNYLQSG
EEEEEEEHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
AEIDLLMTSFEIPGVSGIQLCWEARLIASSGRPLYVIAMSSNNDADHLAEALDSGADDFV
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHH
SKPPSPVELEARLRAAERMNLAQRELVRLATRDAMTDLLNRRTFFARAEDMIHNRTKASV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ILLDVDLLRHINETHGYPGGDTVVMKVAQSMKRYQGMAARIGGEEFAVLLPGLSEAEAVE
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHH
EGERLRWSIADQAVTMPNGQLITVTASLGVAERKPDDNVDEWMESADIALYAAKAGGRNR
CCCCEEEEECCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCE
VVMSEEIMDPDAPRAGLARGGGGRALRGG
EEEEHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLLQTCPRVSTLATFCHIAETCVVTVCPAHIRQYGNIAPASSPEWRQIKGGLYGAAHPG
CCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCC
RVTVQLTTVLCFHELGPMRIMLVDASRVTLKLLAKTLLESRHDVVAFSDGGEALNYLQSG
EEEEEEEHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
AEIDLLMTSFEIPGVSGIQLCWEARLIASSGRPLYVIAMSSNNDADHLAEALDSGADDFV
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHH
SKPPSPVELEARLRAAERMNLAQRELVRLATRDAMTDLLNRRTFFARAEDMIHNRTKASV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ILLDVDLLRHINETHGYPGGDTVVMKVAQSMKRYQGMAARIGGEEFAVLLPGLSEAEAVE
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHH
EGERLRWSIADQAVTMPNGQLITVTASLGVAERKPDDNVDEWMESADIALYAAKAGGRNR
CCCCEEEEECCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCE
VVMSEEIMDPDAPRAGLARGGGGRALRGG
EEEEHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA