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The map label for this gene is queA

Identifier: 158423932

GI number: 158423932

Start: 2632826

End: 2633890

Strand: Reverse

Name: queA

Synonym: AZC_2308

Alternate gene names: 158423932

Gene position: 2633890-2632826 (Counterclockwise)

Preceding gene: 158423933

Following gene: 158423931

Centisome position: 49.05

GC content: 68.83

Gene sequence:

>1065_bases
ATGCGCGTCGACGCCTTCGATTTCGACCTTCCCCCCGAACGCATCGCTTTGCGCCCCGCCGAACCGCGGGAGAGTGCGCG
CCTGCTGCGCGTGCGTCCCGGCGCAGCGCCGGAACTGGAAGACCGCACCATCGCCGACCTTCCGGATCTGCTCCAGCCGG
GCGACGCCTTGGTGGTCAATGACACGAAAGTGATCCCGGCCCGGCTCGATGGCACGCGCACCCGTGCCGGCGGCAGCACG
GTGGCCATCGAGGCGACTCTCATCCGCCGCCTCTCGGGTTCGGCCTGGGCCGCCTTCGCCAAGCCCGCCAAGCGCCTCGC
GGTGGGCGAGACCGTGGTCTTTGCCGGCGCGAGCGGCGGCACTTTGGAGGCCCGTGTTCTCGACAAGCTGGAGAGCGGCG
AGGTGCATTTCGCCTTTGCCCTGTCCGGCCCTGAGCTGGATGCGGCCATCGACGCCATCGGTCACATGCCGCTGCCGCCC
TACATCGCCGCCAAGCGGCCCGAAGACGATCGCGACCGCACCGACTACCAGCCGGTGTTCGCCCGCGTGGAGGGCTCGGT
CGCTGCGCCGACGGCGAGCCTGCATTTCACGCCCGACCTGCTGGCGAAGATCGCGGCGCGCGGCGTCTCCCGCTACACGG
TCACGCTGCATGTGGGGGCGGGCACCTTCCTGCCGGTGAAGGCCGAGGACACCACCGACCACCAGATGCATGCGGAATGG
GGCGAGGTGAGCCCCGAAACGGCGGCGGAGCTCAATGCGGTGAAGGCCCGGGGCGGGCGCATCATCACCGTGGGCTCAAC
CGCTACCCGCCTCATCGAGACCGCGGCGACGCCGGAGGGGTCCATCCGCCCCTATCTCGGCGAGACGGACATCTTCATCA
CGCCCGGCTATGCCTTCCGTGCCTCCGATGTGATGCTCACCAATTTCCACCTGCCGAGATCGACGCTGGTGATGCTGGTG
GCTGCCTTCGTGGGACACGAGACCCAGAAGCGCGCTTACGCCCACGCCATCGCCTCCGGCTATCGCTTCTATTCCTACGG
CGACGCCTGCCTACTGGAACGGTGA

Upstream 100 bases:

>100_bases
GCGGTCGCCATCTTCTGACGGCCCTTTTCCTCCGCGCGCATGCCCGCTAAGAGCGGGCGCCCAACGCAGCCGGGCCTCTG
GCGAGGCCCCTCGAGGACCC

Downstream 100 bases:

>100_bases
GGTTGCCATACGTGCGGCGCGCTTTTCATTCGGGCCGGCCCGTGCTCTAGAGCAGCCGAAATGACCGACACCTTCTCCTT
CAAGGTCCACGCGACCGACG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRVDAFDFDLPPERIALRPAEPRESARLLRVRPGAAPELEDRTIADLPDLLQPGDALVVNDTKVIPARLDGTRTRAGGST
VAIEATLIRRLSGSAWAAFAKPAKRLAVGETVVFAGASGGTLEARVLDKLESGEVHFAFALSGPELDAAIDAIGHMPLPP
YIAAKRPEDDRDRTDYQPVFARVEGSVAAPTASLHFTPDLLAKIAARGVSRYTVTLHVGAGTFLPVKAEDTTDHQMHAEW
GEVSPETAAELNAVKARGGRIITVGSTATRLIETAATPEGSIRPYLGETDIFITPGYAFRASDVMLTNFHLPRSTLVMLV
AAFVGHETQKRAYAHAIASGYRFYSYGDACLLER

Sequences:

>Translated_354_residues
MRVDAFDFDLPPERIALRPAEPRESARLLRVRPGAAPELEDRTIADLPDLLQPGDALVVNDTKVIPARLDGTRTRAGGST
VAIEATLIRRLSGSAWAAFAKPAKRLAVGETVVFAGASGGTLEARVLDKLESGEVHFAFALSGPELDAAIDAIGHMPLPP
YIAAKRPEDDRDRTDYQPVFARVEGSVAAPTASLHFTPDLLAKIAARGVSRYTVTLHVGAGTFLPVKAEDTTDHQMHAEW
GEVSPETAAELNAVKARGGRIITVGSTATRLIETAATPEGSIRPYLGETDIFITPGYAFRASDVMLTNFHLPRSTLVMLV
AAFVGHETQKRAYAHAIASGYRFYSYGDACLLER
>Mature_354_residues
MRVDAFDFDLPPERIALRPAEPRESARLLRVRPGAAPELEDRTIADLPDLLQPGDALVVNDTKVIPARLDGTRTRAGGST
VAIEATLIRRLSGSAWAAFAKPAKRLAVGETVVFAGASGGTLEARVLDKLESGEVHFAFALSGPELDAAIDAIGHMPLPP
YIAAKRPEDDRDRTDYQPVFARVEGSVAAPTASLHFTPDLLAKIAARGVSRYTVTLHVGAGTFLPVKAEDTTDHQMHAEW
GEVSPETAAELNAVKARGGRIITVGSTATRLIETAATPEGSIRPYLGETDIFITPGYAFRASDVMLTNFHLPRSTLVMLV
AAFVGHETQKRAYAHAIASGYRFYSYGDACLLER

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=42.2222222222222, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_AZOC5 (A8I5Q2)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525224.1
- ProteinModelPortal:   A8I5Q2
- SMR:   A8I5Q2
- GeneID:   5689591
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2308
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   ACAU438753:AZC_2308-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38052; Mature: 38052

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDAFDFDLPPERIALRPAEPRESARLLRVRPGAAPELEDRTIADLPDLLQPGDALVVN
CCCCCCCCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCCCCHHCCHHHHCCCCEEEEE
DTKVIPARLDGTRTRAGGSTVAIEATLIRRLSGSAWAAFAKPAKRLAVGETVVFAGASGG
CCEEEEEECCCCEECCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCC
TLEARVLDKLESGEVHFAFALSGPELDAAIDAIGHMPLPPYIAAKRPEDDRDRTDYQPVF
EEHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEE
ARVEGSVAAPTASLHFTPDLLAKIAARGVSRYTVTLHVGAGTFLPVKAEDTTDHQMHAEW
EEECCCEECCCEEEEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCHHHCCC
GEVSPETAAELNAVKARGGRIITVGSTATRLIETAATPEGSIRPYLGETDIFITPGYAFR
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEE
ASDVMLTNFHLPRSTLVMLVAAFVGHETQKRAYAHAIASGYRFYSYGDACLLER
ECCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCEEEECC
>Mature Secondary Structure
MRVDAFDFDLPPERIALRPAEPRESARLLRVRPGAAPELEDRTIADLPDLLQPGDALVVN
CCCCCCCCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCCCCHHCCHHHHCCCCEEEEE
DTKVIPARLDGTRTRAGGSTVAIEATLIRRLSGSAWAAFAKPAKRLAVGETVVFAGASGG
CCEEEEEECCCCEECCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCC
TLEARVLDKLESGEVHFAFALSGPELDAAIDAIGHMPLPPYIAAKRPEDDRDRTDYQPVF
EEHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEE
ARVEGSVAAPTASLHFTPDLLAKIAARGVSRYTVTLHVGAGTFLPVKAEDTTDHQMHAEW
EEECCCEECCCEEEEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCHHHCCC
GEVSPETAAELNAVKARGGRIITVGSTATRLIETAATPEGSIRPYLGETDIFITPGYAFR
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEE
ASDVMLTNFHLPRSTLVMLVAAFVGHETQKRAYAHAIASGYRFYSYGDACLLER
ECCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA