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The map label for this gene is nuoN

Identifier: 158423305

GI number: 158423305

Start: 1922930

End: 1924360

Strand: Direct

Name: nuoN

Synonym: AZC_1681

Alternate gene names: 158423305

Gene position: 1922930-1924360 (Clockwise)

Preceding gene: 158423304

Following gene: 158423306

Centisome position: 35.81

GC content: 66.39

Gene sequence:

>1431_bases
ATGTCCTCCCTCCTGCCGCCTCTCGGCGCGGTCCTCCCGGAACTGCTCCTCGCGCTCTCCGCCGTGGTGCTGGTGCTGAT
CGGCGCCATCCAGGGTGAAAAGTCGGCCAATCTGGTGAACGGCCTCGCCATCGCGGCGCTGGTGGCCGCCGGGGTGCTCG
TGATGCTCCAGCCGGCGGTGACCATCGCCGGCTTCAACGGTTCCATGCTGGTCGATCCCTTCGCCCGCTTCATGAAGGTG
GTGGCGCTGCTCGGTGCGGCGGTCTCCCTCATCATGTCGGTGGACTGGCTGAACCGCGCCCAGCAGGCGAAGTTCGAATA
TGCCGTCCTGGTCGTCCTTGCCTCGCTCGGCATCTGCATCCTGATCTCGGCCGGCGACCTCATCGCCCTCTATCTCGGCC
TCGAGCTGATGAGCCTTTCGCTCTATGTGGTCGCCGCCATCAACCGCGACAGCGTGCGCTCCACGGAGGCGGGCCTGAAG
TATTTCGTGCTGGGCGCCCTGTCCTCCGGCATGTTGCTCTACGGCGCCTCGCTCATCTACGGCTTCACCGGCACGGTGAA
CTTCGCCGGCATTGCCAAGGTCGCCACCGCCCCGACCACGGGCCTCGTGTTCGGCATCGTCTTCCTGTTCGCCGGCCTGT
GCTTCAAGGTCTCGGCCGTGCCGTTCCACATGTGGACACCGGACGTGTACGAAGGCGCGCCCACCCCGGTCACCGCCTTC
TTCGCCACCGCCCCGAAGGTTGCCGCCATGGCCGTTTTCGTCCGCGTGGCCGTCGAGGCGCTGCCGCACGTGACGCACTC
CTGGCAGCAGATCGTCACCTTCGTCTCCATCGCCTCCATGGCGCTCGGCGCGTTCGCCGCCATCGGCCAGCGCAACATCA
AGCGCCTTCTCGCCTATTCCTCCATCGGCCACATGGGCTTCGCGCTCGTGGGTTTGGCGGCGGGCACGGAGCAGGGCGTG
ACCGGCGTGCTGCTCTACATGGCCATCTATGTGGTGATGACGCTCGGCTCCTTCACCTGCGTGCTGGCTATGCGCCGCGA
CGGCCGCTCGGTGGAGACCATCGAGGATCTCGCCGGCCTGGCCCGCACCAAGCCGCTCATGGCGCTCGCGCTCGCCGCGC
TCATGTTCTCGCTGGCGGGCATTCCGCCGCTGGCTGGATTCGTTGCAAAGTACTATGTGTTTCTGGCGGCGATCCAGGCG
GGGCTGTATGGTCTGGCCGTGATCGGTGTGGTGGCGTCGGTGGTCGGCGCCTACTACTATCTCCGTGTGGTCAAGATCAT
GTACTTTGACGAACCGGCCGAGGCGTTCGACGGCATGCCCGGCGAACTGAAGGCGGTGCTGGCCGTGTCCGGCCTGTTCA
CCACCTTCTTCTTCCTCGCCCCGACTCCTCTTATTGCGGCTGCGGGCGCTGCGGCGCGCGCGCTTTTCTGA

Upstream 100 bases:

>100_bases
GTCCCACGCGGTGAACTTCGTCGTCGCCCGTACCGAGACTGCCGCGAGCGTCGCCAAGACCGCCGCGCTGCTGCTCGGCC
ACTGATCCAGAGGTCGTCCG

Downstream 100 bases:

>100_bases
ACGATGGCGGAACAAAGGTTTCAAAGCTCGACCATCCCCATCATCCGCTTCACGGAGATCGGTTCGACCAATGCGGAGGG
TCTGGCGCGCCTGAGCCGGG

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSSLLPPLGAVLPELLLALSAVVLVLIGAIQGEKSANLVNGLAIAALVAAGVLVMLQPAVTIAGFNGSMLVDPFARFMKV
VALLGAAVSLIMSVDWLNRAQQAKFEYAVLVVLASLGICILISAGDLIALYLGLELMSLSLYVVAAINRDSVRSTEAGLK
YFVLGALSSGMLLYGASLIYGFTGTVNFAGIAKVATAPTTGLVFGIVFLFAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
FATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVSIASMALGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTEQGV
TGVLLYMAIYVVMTLGSFTCVLAMRRDGRSVETIEDLAGLARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLAAIQA
GLYGLAVIGVVASVVGAYYYLRVVKIMYFDEPAEAFDGMPGELKAVLAVSGLFTTFFFLAPTPLIAAAGAAARALF

Sequences:

>Translated_476_residues
MSSLLPPLGAVLPELLLALSAVVLVLIGAIQGEKSANLVNGLAIAALVAAGVLVMLQPAVTIAGFNGSMLVDPFARFMKV
VALLGAAVSLIMSVDWLNRAQQAKFEYAVLVVLASLGICILISAGDLIALYLGLELMSLSLYVVAAINRDSVRSTEAGLK
YFVLGALSSGMLLYGASLIYGFTGTVNFAGIAKVATAPTTGLVFGIVFLFAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
FATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVSIASMALGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTEQGV
TGVLLYMAIYVVMTLGSFTCVLAMRRDGRSVETIEDLAGLARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLAAIQA
GLYGLAVIGVVASVVGAYYYLRVVKIMYFDEPAEAFDGMPGELKAVLAVSGLFTTFFFLAPTPLIAAAGAAARALF
>Mature_475_residues
SSLLPPLGAVLPELLLALSAVVLVLIGAIQGEKSANLVNGLAIAALVAAGVLVMLQPAVTIAGFNGSMLVDPFARFMKVV
ALLGAAVSLIMSVDWLNRAQQAKFEYAVLVVLASLGICILISAGDLIALYLGLELMSLSLYVVAAINRDSVRSTEAGLKY
FVLGALSSGMLLYGASLIYGFTGTVNFAGIAKVATAPTTGLVFGIVFLFAGLCFKVSAVPFHMWTPDVYEGAPTPVTAFF
ATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVSIASMALGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTEQGVT
GVLLYMAIYVVMTLGSFTCVLAMRRDGRSVETIEDLAGLARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLAAIQAG
LYGLAVIGVVASVVGAYYYLRVVKIMYFDEPAEAFDGMPGELKAVLAVSGLFTTFFFLAPTPLIAAAGAAARALF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=294, Percent_Identity=22.7891156462585, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI145693160, Length=471, Percent_Identity=34.6072186836518, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788614, Length=365, Percent_Identity=25.4794520547945, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788831, Length=295, Percent_Identity=25.4237288135593, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788827, Length=278, Percent_Identity=25.5395683453237, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788613, Length=321, Percent_Identity=23.6760124610592, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788829, Length=322, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_AZOC5 (A8I421)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524597.1
- GeneID:   5691271
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1681
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- ProtClustDB:   PRK05777
- BioCyc:   ACAU438753:AZC_1681-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 49610; Mature: 49479

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c1ae47c)-; HASH(0x1fd85c00)-; HASH(0x1f7c55f4)-; HASH(0x1fd85918)-; HASH(0x1fd72e3c)-; HASH(0x1fd8590c)-; HASH(0x1fd72c20)-; HASH(0x1fd85aec)-; HASH(0x1ee3593c)-; HASH(0x1fd72b90)-; HASH(0x1f7c55e8)-; HASH(0x1fd72cd4)-; HASH(0x1f7c581c)-; HASH(0x1fd72d1c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLPPLGAVLPELLLALSAVVLVLIGAIQGEKSANLVNGLAIAALVAAGVLVMLQPAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIAGFNGSMLVDPFARFMKVVALLGAAVSLIMSVDWLNRAQQAKFEYAVLVVLASLGICI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LISAGDLIALYLGLELMSLSLYVVAAINRDSVRSTEAGLKYFVLGALSSGMLLYGASLIY
EECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GFTGTVNFAGIAKVATAPTTGLVFGIVFLFAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCHHHH
FATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVSIASMALGAFAAIGQRNIKRLLAYS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGHMGFALVGLAAGTEQGVTGVLLYMAIYVVMTLGSFTCVLAMRRDGRSVETIEDLAGL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLAAIQAGLYGLAVIGVVASVVGAYYY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRVVKIMYFDEPAEAFDGMPGELKAVLAVSGLFTTFFFLAPTPLIAAAGAAARALF
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCH
>Mature Secondary Structure 
SSLLPPLGAVLPELLLALSAVVLVLIGAIQGEKSANLVNGLAIAALVAAGVLVMLQPAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIAGFNGSMLVDPFARFMKVVALLGAAVSLIMSVDWLNRAQQAKFEYAVLVVLASLGICI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LISAGDLIALYLGLELMSLSLYVVAAINRDSVRSTEAGLKYFVLGALSSGMLLYGASLIY
EECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GFTGTVNFAGIAKVATAPTTGLVFGIVFLFAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCHHHH
FATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVSIASMALGAFAAIGQRNIKRLLAYS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGHMGFALVGLAAGTEQGVTGVLLYMAIYVVMTLGSFTCVLAMRRDGRSVETIEDLAGL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLAAIQAGLYGLAVIGVVASVVGAYYY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRVVKIMYFDEPAEAFDGMPGELKAVLAVSGLFTTFFFLAPTPLIAAAGAAARALF
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA