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The map label for this gene is nqo13 [H]

Identifier: 158423304

GI number: 158423304

Start: 1921400

End: 1922914

Strand: Direct

Name: nqo13 [H]

Synonym: AZC_1680

Alternate gene names: 158423304

Gene position: 1921400-1922914 (Clockwise)

Preceding gene: 158423303

Following gene: 158423305

Centisome position: 35.78

GC content: 64.36

Gene sequence:

>1515_bases
ATGTCCAACTGGCCGATCCTCTCCGTCGTCACCTTCCTGCCGCTGGTGGGTGCGCTGTTCATCTTCGTCGTGCGCGGCGA
GGATGAGGTCGCGGCGCGCAACGCCCGCTGGGTGGCGCTGTGGACCACGCTCGTCACCTTCGTGGTGTCGCTCTTCCTGC
TGCCGGGCTTTGATCCGGCGGCGCCCGGCTTCCAGTTCGTGGAGAAGAAGCCCTGGCTCGGCGGCATCGCCGCCTATCAC
ATGGGCGTCGACGGCATCTCGCTGTCGCTGGTGATCCTGTCGACCTTCCTCATGCCTTTCTGCATCCTGGCCTCCTGGGA
CAGCGTGAAGAAGCGCGTGAAGGAGTACATGATCTGCTTCCTGGTGCTCGAGACCCTGATGGTCGGCACCTTCTGCGCCC
TCGACCTCGTCCTCTTCTACTTCTTCTTCGAGGCGGCCCTCATCCCGATGTTCCTCATCATCGGTGTGTGGGGCGGCGCG
CGGCGCATCTACGCCTCGTTCAAGTTCTTCCTCTACACGCTGCTCGGCTCCGTGCTCATGATGCTCGCCATCATGGCCAT
GTACTGGAAAGCCGGTACCACCGACGTGCCCACCCTGATGCAGTACGGCTTCCCGGCCGGCATGCAGACCTGGCTGTGGC
TCGCCTTCTTCGCCTCCTTCGCGGTGAAGATGCCCATGTGGCCGGTGCACACCTGGCTGCCCGACGCGCACGTGGAGGCG
CCGACCGCCGGCTCCGTGATCCTGGCGGGCGTGCTGCTGAAGATGGGCGGCTACGGCTTCATCCGCTTCTCGCTGCCCAT
GTTCCCGGATGCGTCGCACCTCTTCGCGCCGCTGGTCTACACGCTCTCCGTGGTGGCCATCATCTACACCTCGCTGGTGG
CGATGGTGCAGGAGGACATCAAGAAGCTCATCGCCTACTCGTCCATCGCCCACATGGGCTTCGTGACGATGGGCCTGTTC
TCGATGAACGAGGCGGGCCTCCAGGGCGCCATGTTCCTGATGATCTCGCACGGCTTCGTGTCGGGTGCGCTCTTCCTCTG
CGTCGGCGTGATCTATGACCGGATGCACACCCGCGAGATCGCGGCCTATGGGGGCCTCGTGAAGCGCATGCCGCTCTATG
CGGTCGCCATGATGGTCTTCACCATGGCCAACGTCGGCCTGCCGGGCACCTCCGGTTTCGTCGGCGAATTCCTCACCATG
CTCTCCGCCTTCCAGCGCAATACCTGGGTCGCCTTCTTCGCCGCCACCGGCGTGATCCTGTCGGCCGCCTATGCGCTGTG
GCTCTACTGGCGGGTCATCTTCGGCCCGCTGGAGAAGGAGAACCTGATGAAGCTCAAGGATCTCAATGCCCGCGAAATCG
GCATCATGATCCCGCTCATCGCGCTCACCATCATCTTCGGCTTCTGGCCGGCGCCCGTGCTGGACATGACGTCCCACGCG
GTGAACTTCGTCGTCGCCCGTACCGAGACTGCCGCGAGCGTCGCCAAGACCGCCGCGCTGCTGCTCGGCCACTGA

Upstream 100 bases:

>100_bases
TGGTCCGGCTGCAGACCGGCTACCTCTACCACTATGCGTTCGTGATGCTGGTCGGCGTTGCCGCCATCATCACCTGGTTC
ATGTTCGCCGGAGTGTGACG

Downstream 100 bases:

>100_bases
TCCAGAGGTCGTCCGATGTCCTCCCTCCTGCCGCCTCTCGGCGCGGTCCTCCCGGAACTGCTCCTCGCGCTCTCCGCCGT
GGTGCTGGTGCTGATCGGCG

Product: proton-translocating NADH-quinone oxidoreductase chain M protein

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSNWPILSVVTFLPLVGALFIFVVRGEDEVAARNARWVALWTTLVTFVVSLFLLPGFDPAAPGFQFVEKKPWLGGIAAYH
MGVDGISLSLVILSTFLMPFCILASWDSVKKRVKEYMICFLVLETLMVGTFCALDLVLFYFFFEAALIPMFLIIGVWGGA
RRIYASFKFFLYTLLGSVLMMLAIMAMYWKAGTTDVPTLMQYGFPAGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKMGGYGFIRFSLPMFPDASHLFAPLVYTLSVVAIIYTSLVAMVQEDIKKLIAYSSIAHMGFVTMGLF
SMNEAGLQGAMFLMISHGFVSGALFLCVGVIYDRMHTREIAAYGGLVKRMPLYAVAMMVFTMANVGLPGTSGFVGEFLTM
LSAFQRNTWVAFFAATGVILSAAYALWLYWRVIFGPLEKENLMKLKDLNAREIGIMIPLIALTIIFGFWPAPVLDMTSHA
VNFVVARTETAASVAKTAALLLGH

Sequences:

>Translated_504_residues
MSNWPILSVVTFLPLVGALFIFVVRGEDEVAARNARWVALWTTLVTFVVSLFLLPGFDPAAPGFQFVEKKPWLGGIAAYH
MGVDGISLSLVILSTFLMPFCILASWDSVKKRVKEYMICFLVLETLMVGTFCALDLVLFYFFFEAALIPMFLIIGVWGGA
RRIYASFKFFLYTLLGSVLMMLAIMAMYWKAGTTDVPTLMQYGFPAGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKMGGYGFIRFSLPMFPDASHLFAPLVYTLSVVAIIYTSLVAMVQEDIKKLIAYSSIAHMGFVTMGLF
SMNEAGLQGAMFLMISHGFVSGALFLCVGVIYDRMHTREIAAYGGLVKRMPLYAVAMMVFTMANVGLPGTSGFVGEFLTM
LSAFQRNTWVAFFAATGVILSAAYALWLYWRVIFGPLEKENLMKLKDLNAREIGIMIPLIALTIIFGFWPAPVLDMTSHA
VNFVVARTETAASVAKTAALLLGH
>Mature_503_residues
SNWPILSVVTFLPLVGALFIFVVRGEDEVAARNARWVALWTTLVTFVVSLFLLPGFDPAAPGFQFVEKKPWLGGIAAYHM
GVDGISLSLVILSTFLMPFCILASWDSVKKRVKEYMICFLVLETLMVGTFCALDLVLFYFFFEAALIPMFLIIGVWGGAR
RIYASFKFFLYTLLGSVLMMLAIMAMYWKAGTTDVPTLMQYGFPAGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEAP
TAGSVILAGVLLKMGGYGFIRFSLPMFPDASHLFAPLVYTLSVVAIIYTSLVAMVQEDIKKLIAYSSIAHMGFVTMGLFS
MNEAGLQGAMFLMISHGFVSGALFLCVGVIYDRMHTREIAAYGGLVKRMPLYAVAMMVFTMANVGLPGTSGFVGEFLTML
SAFQRNTWVAFFAATGVILSAAYALWLYWRVIFGPLEKENLMKLKDLNAREIGIMIPLIALTIIFGFWPAPVLDMTSHAV
NFVVARTETAASVAKTAALLLGH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=351, Percent_Identity=34.7578347578348, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI251831117, Length=246, Percent_Identity=27.2357723577236, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788613, Length=487, Percent_Identity=36.5503080082135, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788827, Length=259, Percent_Identity=30.8880308880309, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788831, Length=293, Percent_Identity=27.6450511945392, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788614, Length=416, Percent_Identity=26.6826923076923, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788829, Length=400, Percent_Identity=25.5, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693160, Length=354, Percent_Identity=24.5762711864407, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI2367154, Length=259, Percent_Identity=27.4131274131274, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55702; Mature: 55571

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNWPILSVVTFLPLVGALFIFVVRGEDEVAARNARWVALWTTLVTFVVSLFLLPGFDPA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
APGFQFVEKKPWLGGIAAYHMGVDGISLSLVILSTFLMPFCILASWDSVKKRVKEYMICF
CCCHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LVLETLMVGTFCALDLVLFYFFFEAALIPMFLIIGVWGGARRIYASFKFFLYTLLGSVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MLAIMAMYWKAGTTDVPTLMQYGFPAGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGVLLKMGGYGFIRFSLPMFPDASHLFAPLVYTLSVVAIIYTSLVAMVQEDI
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSIAHMGFVTMGLFSMNEAGLQGAMFLMISHGFVSGALFLCVGVIYDRMHTREI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYGGLVKRMPLYAVAMMVFTMANVGLPGTSGFVGEFLTMLSAFQRNTWVAFFAATGVIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SAAYALWLYWRVIFGPLEKENLMKLKDLNAREIGIMIPLIALTIIFGFWPAPVLDMTSHA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VNFVVARTETAASVAKTAALLLGH
HHHHEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNWPILSVVTFLPLVGALFIFVVRGEDEVAARNARWVALWTTLVTFVVSLFLLPGFDPA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
APGFQFVEKKPWLGGIAAYHMGVDGISLSLVILSTFLMPFCILASWDSVKKRVKEYMICF
CCCHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LVLETLMVGTFCALDLVLFYFFFEAALIPMFLIIGVWGGARRIYASFKFFLYTLLGSVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MLAIMAMYWKAGTTDVPTLMQYGFPAGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGVLLKMGGYGFIRFSLPMFPDASHLFAPLVYTLSVVAIIYTSLVAMVQEDI
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSIAHMGFVTMGLFSMNEAGLQGAMFLMISHGFVSGALFLCVGVIYDRMHTREI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYGGLVKRMPLYAVAMMVFTMANVGLPGTSGFVGEFLTMLSAFQRNTWVAFFAATGVIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SAAYALWLYWRVIFGPLEKENLMKLKDLNAREIGIMIPLIALTIIFGFWPAPVLDMTSHA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VNFVVARTETAASVAKTAALLLGH
HHHHEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]