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The map label for this gene is proP [H]

Identifier: 158423113

GI number: 158423113

Start: 1689818

End: 1691116

Strand: Direct

Name: proP [H]

Synonym: AZC_1489

Alternate gene names: 158423113

Gene position: 1689818-1691116 (Clockwise)

Preceding gene: 158423111

Following gene: 158423114

Centisome position: 31.47

GC content: 68.36

Gene sequence:

>1299_bases
ATGGCAAAGACGCTTGCGCGCTCGGGGCAGGGTGCCGCCGCGCCCGAAGGTGAAGGACGCCGCGCCGTTGCGGCGGCGGT
CATCGGCAACATCCTCGAATGGTACGACTTCGCCATCTACGGCTATGTCGCCACCATCATCGCGCACAAGTTCTTCCCGC
CGGGCGACGAGGTGAGCGGCCTGCTCGCCACCTTCGCCACCTTCGGCATCGGTTTTGCCGCCCGCCCCCTCGGCGGCATC
CTCATTGGCCGGATGGGCGACACCCGCGGCCGCAAGGCTGCGCTCCTGCTCACCATCTTCACCATGGCCATTGGCACCGT
GGGCATCGGCCTCATTCCCTCCTATGAGGTCATCGGCGTGCTGGCGCCGGTGCTGCTGGTGCTCTGCCGCCTGTTGCAGG
GCTTCGCCGCCGGCGGCGAATGGGGCGGCGCCACCGCCTTCATCGTGGAATGGGCGCCCAGGAACCGGCGCGGCTATTTC
GGCAGTTGGCAGCAGGCGAGTGTCGCCGGCGGCCTGCTCCTCGGCTCGGGCACCGCCGCCCTGTTCAGCACGCTGCTGAC
GCCGGACCAGATGGACGCCTGGGGCTGGCGCATCCCCTTCATCCTCGGCATCATCCTGCTGCCGGTCGGCCTCTACATGC
GGGCGAACATCGACGAGACGCCCGCCTATCGCGAGGCCCGGGCCGAGCCCGTGGCGCAGGCCCGCGTGCGGACACCGGGC
TGGGTGCTGGCCGCCAAGGCGTTCGGCTTCACCATCCTCTGGACGGTGTCCTATTATGTGATCCTCTATTACATGCCCAC
CTTCACCCAGAAGTATGCCGGCCTCAGCCGCACCGAGTCCCTGTGGTCGAACACGCTCGGGCTCGTGGTGCTGGCGGTGG
TGATTCCGCTCATGGGCCTGTTGTCCGACCGCATCGGCCGCAAGCCCATGCTGCTCGCCTGCTGCGTGGCGTTCGTGGTG
CTCACCTATCCGCTGTTCGCGGCGATGTCGGGCGGCGCGCCGCTGCTCACCGTGATGGCGATCCAGATCGTGTTCGGCAT
CATGATCGCCATGTTCTCCGGGCCCGGCCCCGCCGCCATCGCCGAGATATTCCCGACCCACTCGCGCTCCACATGGATGT
CCACGGGCTACAGCCTGGCGGTGACGGTGTTCGGCGGCTTTGCGCCCTTCATCGCCACCTGGCTGATCAGCACCACGGGC
TCGCCGCTGGCGCCGACCTTCTATCTGATCGCCGCCGGCGTGGTGTCGGCCGTGGTCATCGCCCTGCTGCCGGAGACGGC
GCACGACGATCTGGCCTGA

Upstream 100 bases:

>100_bases
TCTCTCCCGGTACGATTTTAAGTATTACTATGCAATACGCTAGATCGTAATGCAATTAGTGGTGCGCACGCACCGGCAAT
AACAAGCAGAGGGGTCCATC

Downstream 100 bases:

>100_bases
CGGACCCGGCCTCCGCCCGGCGGCGGGGGCCATCCCACTTCGGGAGGCTGAGATGACGCTCGACTTCAACGGCAGGACGG
TGGCCGTCTCCGGTGCCGGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAKTLARSGQGAAAPEGEGRRAVAAAVIGNILEWYDFAIYGYVATIIAHKFFPPGDEVSGLLATFATFGIGFAARPLGGI
LIGRMGDTRGRKAALLLTIFTMAIGTVGIGLIPSYEVIGVLAPVLLVLCRLLQGFAAGGEWGGATAFIVEWAPRNRRGYF
GSWQQASVAGGLLLGSGTAALFSTLLTPDQMDAWGWRIPFILGIILLPVGLYMRANIDETPAYREARAEPVAQARVRTPG
WVLAAKAFGFTILWTVSYYVILYYMPTFTQKYAGLSRTESLWSNTLGLVVLAVVIPLMGLLSDRIGRKPMLLACCVAFVV
LTYPLFAAMSGGAPLLTVMAIQIVFGIMIAMFSGPGPAAIAEIFPTHSRSTWMSTGYSLAVTVFGGFAPFIATWLISTTG
SPLAPTFYLIAAGVVSAVVIALLPETAHDDLA

Sequences:

>Translated_432_residues
MAKTLARSGQGAAAPEGEGRRAVAAAVIGNILEWYDFAIYGYVATIIAHKFFPPGDEVSGLLATFATFGIGFAARPLGGI
LIGRMGDTRGRKAALLLTIFTMAIGTVGIGLIPSYEVIGVLAPVLLVLCRLLQGFAAGGEWGGATAFIVEWAPRNRRGYF
GSWQQASVAGGLLLGSGTAALFSTLLTPDQMDAWGWRIPFILGIILLPVGLYMRANIDETPAYREARAEPVAQARVRTPG
WVLAAKAFGFTILWTVSYYVILYYMPTFTQKYAGLSRTESLWSNTLGLVVLAVVIPLMGLLSDRIGRKPMLLACCVAFVV
LTYPLFAAMSGGAPLLTVMAIQIVFGIMIAMFSGPGPAAIAEIFPTHSRSTWMSTGYSLAVTVFGGFAPFIATWLISTTG
SPLAPTFYLIAAGVVSAVVIALLPETAHDDLA
>Mature_431_residues
AKTLARSGQGAAAPEGEGRRAVAAAVIGNILEWYDFAIYGYVATIIAHKFFPPGDEVSGLLATFATFGIGFAARPLGGIL
IGRMGDTRGRKAALLLTIFTMAIGTVGIGLIPSYEVIGVLAPVLLVLCRLLQGFAAGGEWGGATAFIVEWAPRNRRGYFG
SWQQASVAGGLLLGSGTAALFSTLLTPDQMDAWGWRIPFILGIILLPVGLYMRANIDETPAYREARAEPVAQARVRTPGW
VLAAKAFGFTILWTVSYYVILYYMPTFTQKYAGLSRTESLWSNTLGLVVLAVVIPLMGLLSDRIGRKPMLLACCVAFVVL
TYPLFAAMSGGAPLLTVMAIQIVFGIMIAMFSGPGPAAIAEIFPTHSRSTWMSTGYSLAVTVFGGFAPFIATWLISTTGS
PLAPTFYLIAAGVVSAVVIALLPETAHDDLA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=34.9425287356322, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788292, Length=424, Percent_Identity=34.4339622641509, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=35.4166666666667, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788942, Length=408, Percent_Identity=32.5980392156863, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI87082231, Length=220, Percent_Identity=29.0909090909091, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082404, Length=381, Percent_Identity=24.1469816272966, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45804; Mature: 45673

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLARSGQGAAAPEGEGRRAVAAAVIGNILEWYDFAIYGYVATIIAHKFFPPGDEVSG
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLATFATFGIGFAARPLGGILIGRMGDTRGRKAALLLTIFTMAIGTVGIGLIPSYEVIGV
HHHHHHHHHCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LAPVLLVLCRLLQGFAAGGEWGGATAFIVEWAPRNRRGYFGSWQQASVAGGLLLGSGTAA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCEEECCCHHH
LFSTLLTPDQMDAWGWRIPFILGIILLPVGLYMRANIDETPAYREARAEPVAQARVRTPG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHCCCCC
WVLAAKAFGFTILWTVSYYVILYYMPTFTQKYAGLSRTESLWSNTLGLVVLAVVIPLMGL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSDRIGRKPMLLACCVAFVVLTYPLFAAMSGGAPLLTVMAIQIVFGIMIAMFSGPGPAAI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
AEIFPTHSRSTWMSTGYSLAVTVFGGFAPFIATWLISTTGSPLAPTFYLIAAGVVSAVVI
HHHCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALLPETAHDDLA
HHCCCCCCCCCC
>Mature Secondary Structure 
AKTLARSGQGAAAPEGEGRRAVAAAVIGNILEWYDFAIYGYVATIIAHKFFPPGDEVSG
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLATFATFGIGFAARPLGGILIGRMGDTRGRKAALLLTIFTMAIGTVGIGLIPSYEVIGV
HHHHHHHHHCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LAPVLLVLCRLLQGFAAGGEWGGATAFIVEWAPRNRRGYFGSWQQASVAGGLLLGSGTAA
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCEEECCCHHH
LFSTLLTPDQMDAWGWRIPFILGIILLPVGLYMRANIDETPAYREARAEPVAQARVRTPG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHCCCCC
WVLAAKAFGFTILWTVSYYVILYYMPTFTQKYAGLSRTESLWSNTLGLVVLAVVIPLMGL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSDRIGRKPMLLACCVAFVVLTYPLFAAMSGGAPLLTVMAIQIVFGIMIAMFSGPGPAAI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
AEIFPTHSRSTWMSTGYSLAVTVFGGFAPFIATWLISTTGSPLAPTFYLIAAGVVSAVVI
HHHCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALLPETAHDDLA
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]