BacMap

Definition	Azorhizobium caulinodans ORS 571, complete genome.
Accession	NC_009937
Length	5,369,772

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The map label for this gene is entA [C]

Identifier: 158423114

GI number: 158423114

Start: 1691169

End: 1691912

Strand: Direct

Name: entA [C]

Synonym: AZC_1490

Alternate gene names: 158423114

Gene position: 1691169-1691912 (Clockwise)

Preceding gene: 158423113

Following gene: 158423115

Centisome position: 31.49

GC content: 69.76

Gene sequence:

>744_bases
ATGACGCTCGACTTCAACGGCAGGACGGTGGCCGTCTCCGGTGCCGGCATCGGCTTCGGCCGGGCCATTTCGCGGCTGTT
CGCGGACGCCGGAGCACAGGTGTTCGGCTGCGATATCCTCGCGGCGGAGGAGCCGGTGCCCGGCGTCGAGATGGCACGGG
TGGACCTCCTCGACCGGGCCGCGGGCGCGGCCTGGATCTCCGGCATCGAGCAGAAGACCGGCAGGGCGCTCGATGTGCTG
GTCTGCAATGCCGGCGGCGTCGCCGGTCAGGCGGGCAAGCCCGTGGAGGACGTGTCCGACGCCGAATGGGACCGGGTGGT
GGACGTCAATCTCAACGCCGCCTTCACCCTGTGCCGCGCCGTCGCGCCGGCCATGAAGAAGGCGGGGCAGGGGGCCATCG
TCACCATCACGTCCGGTGCCGCCCTGCAGGCCTCGCTGACTGGCGTTCAGGCCTATTGCGCCTCAAAGCATGCCATGCTG
GGACTGACGCGGCAGCTTGCGCACGAGCTGGGACCGTTCGGCATCCGGGTCAATAGCGTGGCGCCGGGCTTCGTGCGCAC
CAATCCCGCCACCGAGCGCCAGTGGGCGGCCTATGGCGCGGAGGGACAGCGTCGGCTGGTGGAGGGCATCGCGTTGAAGC
GGCTCGGCACGGCGGAGGAGATCGCCAAGGTGGTGCTCTTTCTCGCCTCCGATCTCGCGAGCTTCGTGAACGGGCAGGTG
CTGCCGGTGGACGGCGGCAAATAG

Upstream 100 bases:

>100_bases
GTGGTCATCGCCCTGCTGCCGGAGACGGCGCACGACGATCTGGCCTGACGGACCCGGCCTCCGCCCGGCGGCGGGGGCCA
TCCCACTTCGGGAGGCTGAG

Downstream 100 bases:

>100_bases
AAACAGGCGGAACGCCAGATGTCGAAAAGGACAAGCGCATGACCAAGACCGTGGCGAACCCGGCCGGGGAGCCCTGGCAG
TGGGAGGAGGCCCACTGGCG

Product: short-chain dehydrogenase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTLDFNGRTVAVSGAGIGFGRAISRLFADAGAQVFGCDILAAEEPVPGVEMARVDLLDRAAGAAWISGIEQKTGRALDVL
VCNAGGVAGQAGKPVEDVSDAEWDRVVDVNLNAAFTLCRAVAPAMKKAGQGAIVTITSGAALQASLTGVQAYCASKHAML
GLTRQLAHELGPFGIRVNSVAPGFVRTNPATERQWAAYGAEGQRRLVEGIALKRLGTAEEIAKVVLFLASDLASFVNGQV
LPVDGGK

Sequences:

>Translated_247_residues
MTLDFNGRTVAVSGAGIGFGRAISRLFADAGAQVFGCDILAAEEPVPGVEMARVDLLDRAAGAAWISGIEQKTGRALDVL
VCNAGGVAGQAGKPVEDVSDAEWDRVVDVNLNAAFTLCRAVAPAMKKAGQGAIVTITSGAALQASLTGVQAYCASKHAML
GLTRQLAHELGPFGIRVNSVAPGFVRTNPATERQWAAYGAEGQRRLVEGIALKRLGTAEEIAKVVLFLASDLASFVNGQV
LPVDGGK
>Mature_246_residues
TLDFNGRTVAVSGAGIGFGRAISRLFADAGAQVFGCDILAAEEPVPGVEMARVDLLDRAAGAAWISGIEQKTGRALDVLV
CNAGGVAGQAGKPVEDVSDAEWDRVVDVNLNAAFTLCRAVAPAMKKAGQGAIVTITSGAALQASLTGVQAYCASKHAMLG
LTRQLAHELGPFGIRVNSVAPGFVRTNPATERQWAAYGAEGQRRLVEGIALKRLGTAEEIAKVVLFLASDLASFVNGQVL
PVDGGK

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=174, Percent_Identity=37.9310344827586, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI15277342, Length=252, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI19923817, Length=262, Percent_Identity=30.5343511450382, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI66933014, Length=226, Percent_Identity=30.0884955752212, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI5031737, Length=256, Percent_Identity=32.421875, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI126723750, Length=144, Percent_Identity=38.1944444444444, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI10190704, Length=261, Percent_Identity=32.5670498084291, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=31.8965517241379, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4503301, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI40254992, Length=262, Percent_Identity=28.2442748091603, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4504505, Length=197, Percent_Identity=32.48730964467, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI302344755, Length=102, Percent_Identity=42.156862745098, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI33667109, Length=194, Percent_Identity=32.9896907216495, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI126723191, Length=106, Percent_Identity=40.5660377358491, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786812, Length=251, Percent_Identity=36.2549800796813, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=33.8582677165354, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=33.59375, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=34.6456692913386, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082160, Length=259, Percent_Identity=32.4324324324324, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789208, Length=253, Percent_Identity=29.2490118577075, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788459, Length=198, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI2367175, Length=251, Percent_Identity=33.8645418326693, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=34.9206349206349, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790717, Length=261, Percent_Identity=30.2681992337165, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789057, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787820, Length=240, Percent_Identity=25.4166666666667, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=242, Percent_Identity=33.8842975206612, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=30.8943089430894, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17561402, Length=253, Percent_Identity=32.0158102766798, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=32.6693227091633, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=30.5220883534137, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=30.8300395256917, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=32, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=31.4393939393939, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=253, Percent_Identity=28.4584980237154, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=29.3233082706767, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536651, Length=262, Percent_Identity=28.2442748091603, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=30.7984790874525, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=29.368029739777, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=29.0566037735849, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=27.9850746268657, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=29.9242424242424, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561272, Length=253, Percent_Identity=29.6442687747036, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=26.8939393939394, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=28.030303030303, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=200, Percent_Identity=29.5, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=264, Percent_Identity=28.7878787878788, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=28.4090909090909, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=31.6326530612245, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=28.6245353159851, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=30.9644670050761, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=267, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=256, Percent_Identity=27.734375, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=34.251968503937, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24643142, Length=255, Percent_Identity=33.7254901960784, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=34.8837209302326, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=32.8, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=35.9073359073359, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355319, Length=172, Percent_Identity=36.046511627907, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24644337, Length=261, Percent_Identity=34.0996168582375, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571526, Length=180, Percent_Identity=37.7777777777778, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18859837, Length=196, Percent_Identity=30.1020408163265, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21358495, Length=204, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17737361, Length=254, Percent_Identity=29.1338582677165, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25473; Mature: 25342

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDFNGRTVAVSGAGIGFGRAISRLFADAGAQVFGCDILAAEEPVPGVEMARVDLLDRA
CEEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
AGAAWISGIEQKTGRALDVLVCNAGGVAGQAGKPVEDVSDAEWDRVVDVNLNAAFTLCRA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHH
VAPAMKKAGQGAIVTITSGAALQASLTGVQAYCASKHAMLGLTRQLAHELGPFGIRVNSV
HHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
APGFVRTNPATERQWAAYGAEGQRRLVEGIALKRLGTAEEIAKVVLFLASDLASFVNGQV
CCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
LPVDGGK
EECCCCC
>Mature Secondary Structure 
TLDFNGRTVAVSGAGIGFGRAISRLFADAGAQVFGCDILAAEEPVPGVEMARVDLLDRA
EEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
AGAAWISGIEQKTGRALDVLVCNAGGVAGQAGKPVEDVSDAEWDRVVDVNLNAAFTLCRA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHH
VAPAMKKAGQGAIVTITSGAALQASLTGVQAYCASKHAMLGLTRQLAHELGPFGIRVNSV
HHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
APGFVRTNPATERQWAAYGAEGQRRLVEGIALKRLGTAEEIAKVVLFLASDLASFVNGQV
CCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
LPVDGGK
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]