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The map label for this gene is purU [H]

Identifier: 158423000

GI number: 158423000

Start: 1558173

End: 1559117

Strand: Direct

Name: purU [H]

Synonym: AZC_1376

Alternate gene names: 158423000

Gene position: 1558173-1559117 (Clockwise)

Preceding gene: 158422999

Following gene: 158423001

Centisome position: 29.02

GC content: 63.6

Gene sequence:

>945_bases
ATGCTCCAGGAAACCACCTTCAAAGCCGCCGGCAGGCTTGAGGACCACGGTGCCGCCGATGCGGCCAGCTACGTGCTCAC
CGTCTCCTGCACCACCCGCCGGGGCATCGTCGCCGCCATCTCCGGCTTCCTCGCCACTGCCGGCTGCAACATCACGGACT
CTTCCCAGTTCGATGATGCGGACACGGGCCGCTTCTTCATGCGCATCCGCTTCCGCTCGGAGGAGGGCATCGGCCAGGAG
GAACTGGACGCCGCCTTCGCGCCGATCGCCGGCCAGATGGGCATCGATTACCAGCTCCACAATGCGAGCCGGCGCATGAA
GGTGCTGCTGATGGTCTCGCGCTTCGGCCACTGCCTCAACGATCTGCTGTACCGCTGGAAGATCGGCGCGCTGCCCATCG
ACATCGTGGGCGTGGTCTCCAACCATTTCGACTATCAGAAAGTGGTCGTGAACCACGACATTCCCTTCCACTGCATCAAG
GTGACGAAGGAGAACAAGGCGGAGGCGGAAGCCCAGCTCCTGTCCATCGTCGAGCAGACCGGCACGGAACTTGTCGTGCT
CGCCCGCTACATGCAGGTGCTCTCCGATGCGCTGTGCCGGAAGATGTCCGGGCGGATCATCAACATCCACCATTCCTTCC
TGCCGAGCTTCAAGGGCGCCAATCCCTACAAGCAGGCCTATGAGCGCGGCGTGAAGCTCATCGGTGCCACCGCCCATTAC
GTCACCAGCGATCTGGATGAAGGTCCGATCATCGAGCAGGACATCGCCCGCATCACCCACGCCCAGAGCCCGGACGATTA
CGTGAGCATCGGCCGCGACGTGGAGAGCCAGGTGCTGGCCCGCGCCATCCACGCGCACATTCACCACCGCATCTTCATCA
ACGGCAACCGCACCGTGGTCTTTCCGGCCTCGCCCGGCTCATACGCCTCCGAGCGCATGGGCTGA

Upstream 100 bases:

>100_bases
CCAGAAGCGCCTCGCCGCAACCGTCGTGCCCTTCCCCTTCTACGACCCCGAAAAGAAGCGCGTGCGCGCCTGAGCGCGCC
ACAGATCGAAGGAAACAGCC

Downstream 100 bases:

>100_bases
CGGACACGCCACCTCCAAGTCATGCCCGGGCTTGTCCCGGCCATGACAGTCCAAACGCCATTTCACACGCCACAAACCTT
TGACAGAACAGGGGAACAAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLQETTFKAAGRLEDHGAADAASYVLTVSCTTRRGIVAAISGFLATAGCNITDSSQFDDADTGRFFMRIRFRSEEGIGQE
ELDAAFAPIAGQMGIDYQLHNASRRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHCIK
VTKENKAEAEAQLLSIVEQTGTELVVLARYMQVLSDALCRKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHY
VTSDLDEGPIIEQDIARITHAQSPDDYVSIGRDVESQVLARAIHAHIHHRIFINGNRTVVFPASPGSYASERMG

Sequences:

>Translated_314_residues
MLQETTFKAAGRLEDHGAADAASYVLTVSCTTRRGIVAAISGFLATAGCNITDSSQFDDADTGRFFMRIRFRSEEGIGQE
ELDAAFAPIAGQMGIDYQLHNASRRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHCIK
VTKENKAEAEAQLLSIVEQTGTELVVLARYMQVLSDALCRKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHY
VTSDLDEGPIIEQDIARITHAQSPDDYVSIGRDVESQVLARAIHAHIHHRIFINGNRTVVFPASPGSYASERMG
>Mature_314_residues
MLQETTFKAAGRLEDHGAADAASYVLTVSCTTRRGIVAAISGFLATAGCNITDSSQFDDADTGRFFMRIRFRSEEGIGQE
ELDAAFAPIAGQMGIDYQLHNASRRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHCIK
VTKENKAEAEAQLLSIVEQTGTELVVLARYMQVLSDALCRKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHY
VTSDLDEGPIIEQDIARITHAQSPDDYVSIGRDVESQVLARAIHAHIHHRIFINGNRTVVFPASPGSYASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=106, Percent_Identity=37.7358490566038, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869995, Length=106, Percent_Identity=37.7358490566038, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869993, Length=106, Percent_Identity=37.7358490566038, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=40.072202166065, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788846, Length=168, Percent_Identity=27.9761904761905, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=152, Percent_Identity=34.8684210526316, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24582400, Length=170, Percent_Identity=31.7647058823529, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 34720; Mature: 34720

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQETTFKAAGRLEDHGAADAASYVLTVSCTTRRGIVAAISGFLATAGCNITDSSQFDDA
CCCCCCHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
DTGRFFMRIRFRSEEGIGQEELDAAFAPIAGQMGIDYQLHNASRRMKVLLMVSRFGHCLN
CCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
DLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHCIKVTKENKAEAEAQLLSIVEQT
HHHHHHCCCCCCEEHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GTELVVLARYMQVLSDALCRKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHY
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHH
VTSDLDEGPIIEQDIARITHAQSPDDYVSIGRDVESQVLARAIHAHIHHRIFINGNRTVV
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCEEEE
FPASPGSYASERMG
EECCCCCCHHHCCC
>Mature Secondary Structure
MLQETTFKAAGRLEDHGAADAASYVLTVSCTTRRGIVAAISGFLATAGCNITDSSQFDDA
CCCCCCHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
DTGRFFMRIRFRSEEGIGQEELDAAFAPIAGQMGIDYQLHNASRRMKVLLMVSRFGHCLN
CCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
DLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHCIKVTKENKAEAEAQLLSIVEQT
HHHHHHCCCCCCEEHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GTELVVLARYMQVLSDALCRKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHY
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHH
VTSDLDEGPIIEQDIARITHAQSPDDYVSIGRDVESQVLARAIHAHIHHRIFINGNRTVV
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCEEEE
FPASPGSYASERMG
EECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]