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The map label for this gene is dnaN [H]

Identifier: 158422637

GI number: 158422637

Start: 1136837

End: 1137955

Strand: Reverse

Name: dnaN [H]

Synonym: AZC_1013

Alternate gene names: 158422637

Gene position: 1137955-1136837 (Counterclockwise)

Preceding gene: 158422638

Following gene: 158422636

Centisome position: 21.19

GC content: 67.02

Gene sequence:

>1119_bases
ATGAAGGTCACCGTCGAGCGCGCCGAGCTTCTCAAGTCGCTCAGCCATGTCCATCGCGTCGTCGAGCGGCGTAACACCAT
CCCGATTCTCGCCAACGTGCTCATCCGCACCGGCACGAACGGGCTGTCGCTGAAGGCCACCGACCTTGATCTCGAGGTGG
TGGAGACGGTGCCGGCGGAAGTCGGCCAGGACGGCGCGACCACGGTGCCCGCCCACGTGCTCTACGACATCGTGCGCAAG
CTGCCCGACGGGTCGCAGGTGCAGCTGGAAGCCGGCGGTGAGCGCGGCAACCTCACGGTGCGCGCCGGCCGCGCCCGCTT
CACCCTCCAGACCCTGCCGGAAGCCGATTTCCCGGATCTCGCAGCGGGCGACTTCTCCCATTCCTTCAAGGTGAAGGGCG
CGGAATTCCGCCGCCTGGTGGACAAGACCCAGTTCGCCATCTCCACCGAGGAAACCCGCTACTACCTCAACGGCATCTAT
CTGCACGTGGCGGATGCTGCCGGCACCGCCAAGCTGCGGGCGGTCGCCACCGACGGCCACCGCCTCGCCCAGGCCGAGCT
GGATGCGCCCGAGGGTTCCGCCGGCCTGCCGGGCGTGATCGTCCCGCGCAAGACGGTGGCCGAAATCCAGAAGCTGCTGG
AAGACAAGGACGCGGAAGTCACCGTCTCCCTGTCCACCACCAAGATCCGCGTCGCTGTGGGCGCCATCGTGCTCACCTCC
AAGCTGATCGACGGCACCTTCCCGGATTATGGCCGGGTCATCCCGGCGGGCAACGACAAGATCCTGCTGGTGGACAAGTC
CGATTTCGCGGCCGCCGTGGACCGCGTGTCGACAGTCTCCAGCGAGCGCGGCCGGGCGGTGAAGCTGTCCATCCAGGACG
GCAAGCTCACCCTCTCCGTGACCAATCCGGACAGCGGCAGCGCCACCGAGGAGCTCGAGGTGGACTACGCCGCCGAGCCC
CTCGATATCGGCTTCAACAGCCGCTACCTGCTCGACATCACTTCGCAGCTCGAAGGCGACACGGCGGAGCTGAAGCTCGC
TGATCCCGGCTCGCCGACGCTGGTGCGCGACAGCACGAAGACGGATTCGCTCTACGTGCTCATGCCCATGCGGGTGTGA

Upstream 100 bases:

>100_bases
TGCGGCCCGGCAGTTCGTTTCGACGAACCCGCAGGGCCTCGGTGGCGCCAGCCATCGGGCGCATTCTTTTCCTCCGAGCG
AAGAGTCACGGGTCCGGGCC

Downstream 100 bases:

>100_bases
GCCGACCGCCTGCCGTGACCGCGCCGCGCGCCCGCGTCCGCCGCCTCACCCTCTCCCGCTTCCGCAGCTATTCCGCCGCC
CAGATCGAGGTGGCGGACGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIRTGTNGLSLKATDLDLEVVETVPAEVGQDGATTVPAHVLYDIVRK
LPDGSQVQLEAGGERGNLTVRAGRARFTLQTLPEADFPDLAAGDFSHSFKVKGAEFRRLVDKTQFAISTEETRYYLNGIY
LHVADAAGTAKLRAVATDGHRLAQAELDAPEGSAGLPGVIVPRKTVAEIQKLLEDKDAEVTVSLSTTKIRVAVGAIVLTS
KLIDGTFPDYGRVIPAGNDKILLVDKSDFAAAVDRVSTVSSERGRAVKLSIQDGKLTLSVTNPDSGSATEELEVDYAAEP
LDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDSLYVLMPMRV

Sequences:

>Translated_372_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIRTGTNGLSLKATDLDLEVVETVPAEVGQDGATTVPAHVLYDIVRK
LPDGSQVQLEAGGERGNLTVRAGRARFTLQTLPEADFPDLAAGDFSHSFKVKGAEFRRLVDKTQFAISTEETRYYLNGIY
LHVADAAGTAKLRAVATDGHRLAQAELDAPEGSAGLPGVIVPRKTVAEIQKLLEDKDAEVTVSLSTTKIRVAVGAIVLTS
KLIDGTFPDYGRVIPAGNDKILLVDKSDFAAAVDRVSTVSSERGRAVKLSIQDGKLTLSVTNPDSGSATEELEVDYAAEP
LDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDSLYVLMPMRV
>Mature_372_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIRTGTNGLSLKATDLDLEVVETVPAEVGQDGATTVPAHVLYDIVRK
LPDGSQVQLEAGGERGNLTVRAGRARFTLQTLPEADFPDLAAGDFSHSFKVKGAEFRRLVDKTQFAISTEETRYYLNGIY
LHVADAAGTAKLRAVATDGHRLAQAELDAPEGSAGLPGVIVPRKTVAEIQKLLEDKDAEVTVSLSTTKIRVAVGAIVLTS
KLIDGTFPDYGRVIPAGNDKILLVDKSDFAAAVDRVSTVSSERGRAVKLSIQDGKLTLSVTNPDSGSATEELEVDYAAEP
LDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDSLYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=40, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40014; Mature: 40014

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIRTGTNGLSLKATDLDLEVVETVPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHEECCCCCCEEEEECCCHHHHHHCHHH
VGQDGATTVPAHVLYDIVRKLPDGSQVQLEAGGERGNLTVRAGRARFTLQTLPEADFPDL
HCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCCCC
AAGDFSHSFKVKGAEFRRLVDKTQFAISTEETRYYLNGIYLHVADAAGTAKLRAVATDGH
CCCCCCCEEEECCHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLAQAELDAPEGSAGLPGVIVPRKTVAEIQKLLEDKDAEVTVSLSTTKIRVAVGAIVLTS
EEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECEEEEEEHHHHHHHH
KLIDGTFPDYGRVIPAGNDKILLVDKSDFAAAVDRVSTVSSERGRAVKLSIQDGKLTLSV
HHHCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVDYAAEPLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTK
ECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEECCCC
TDSLYVLMPMRV
CCCEEEEEECCC
>Mature Secondary Structure
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIRTGTNGLSLKATDLDLEVVETVPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHEECCCCCCEEEEECCCHHHHHHCHHH
VGQDGATTVPAHVLYDIVRKLPDGSQVQLEAGGERGNLTVRAGRARFTLQTLPEADFPDL
HCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCCCC
AAGDFSHSFKVKGAEFRRLVDKTQFAISTEETRYYLNGIYLHVADAAGTAKLRAVATDGH
CCCCCCCEEEECCHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLAQAELDAPEGSAGLPGVIVPRKTVAEIQKLLEDKDAEVTVSLSTTKIRVAVGAIVLTS
EEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECEEEEEEHHHHHHHH
KLIDGTFPDYGRVIPAGNDKILLVDKSDFAAAVDRVSTVSSERGRAVKLSIQDGKLTLSV
HHHCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVDYAAEPLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTK
ECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEECCCC
TDSLYVLMPMRV
CCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]