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The map label for this gene is recF [H]

Identifier: 158422636

GI number: 158422636

Start: 1135686

End: 1136822

Strand: Reverse

Name: recF [H]

Synonym: AZC_1012

Alternate gene names: 158422636

Gene position: 1136822-1135686 (Counterclockwise)

Preceding gene: 158422637

Following gene: 158422635

Centisome position: 21.17

GC content: 72.74

Gene sequence:

>1137_bases
GTGACCGCGCCGCGCGCCCGCGTCCGCCGCCTCACCCTCTCCCGCTTCCGCAGCTATTCCGCCGCCCAGATCGAGGTGGC
GGACGGTCCGGTGGTGCTCACGGGGCCGAACGGCGCGGGCAAGACCAACATCCTTGAAGCCGTCTCCCTGCTCTCGCCGG
GACGCGGGCTGCGCCGTGCCGCGCTCGATGCCTTCGCGCAGGCGGGCGGCGACGGCTCCTGGGCAGTGGCCGCCCATGTG
GAGGGCGCGCTCGGCCCGGTTGATCTCGGCACGGGCAGCGATGCCAGCCGGCCGGACGGCAGCACCCGGCGCTGCCGCAT
CGACCGCGAGAGTGTGTCGTCCGCCAATGCCTTCCTCGACCATCTCAAGGTGGTGTGGCTGACACCGGAGATGGACGGCC
TGTTCACCGGCCCGCCCGCCGACCGCCGCCGCTTCCTCGATCGCCTCGTGCTGGCGGTGGATGCCTCCCACGGCACGCGG
GTCAACGCATTGGAACGCGCGCTGCGCAGCCGTAACCGCGTGCTGGAGGATGGCGGCGACAATCGCATGCTCTCCGCCGT
CGAGCATGAACTGGCGGAACTGGGTGTTGCCGTCGCCGCTGCCCGGCTGGAAACGGTGCAGCGCCTCGCCGGCGAGATCG
CGGCCCATGCGGACGAGACCTCCCCCTTCCCCCACGCGGACATCGCCCTCGACGGCGCGCTGGAGCGCATGCTGACCGAC
GCGCCCGCCGTCGAGGTGGAAGACCGCTATCGCGCCCTCCTTCAGGACAACCGCCCCCGCGACCGCGCCGCCGGCCGCAC
TCTGGAGGGGCCGCACCTGTGCGACCTCGTGGTGGCCCACGGCCCCAAGGGGCTGCCCGCGGCCCGCTGCTCCACGGGCG
AGCAGAAGTCGCTCCTCATCGGCCTTGCCCTTTCCCACGCCCGGCTCGTCACCGCCATGCAGGGCCTTGCCCCGGTGGTG
CTACTGGACGACGTGGTGGCCTATCTCGACGCCGAGCGGCGCTCCGGCATGTTCGCGGCACTGAAGGCGCTGGGTGCGCA
GGTGTGGATGACCGGGGCCGACCCGTCCGCCTTCGCGGCGCTGGACGGCGCCGAGCGCTTCACCGTCAGCCCCGGTGCGG
TGAACAGGAGCGTTTGA

Upstream 100 bases:

>100_bases
AGCTCGCTGATCCCGGCTCGCCGACGCTGGTGCGCGACAGCACGAAGACGGATTCGCTCTACGTGCTCATGCCCATGCGG
GTGTGAGCCGACCGCCTGCC

Downstream 100 bases:

>100_bases
CGGCCGTGTGATCGCCAAAATGGCGGCTTTTCGGACGGGTGTTGCCGCTGTGCAACCAGCCCCGGAAACCCGCGTGCTAG
AAGGCGAATCAGACCTCTCC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTAPRARVRRLTLSRFRSYSAAQIEVADGPVVLTGPNGAGKTNILEAVSLLSPGRGLRRAALDAFAQAGGDGSWAVAAHV
EGALGPVDLGTGSDASRPDGSTRRCRIDRESVSSANAFLDHLKVVWLTPEMDGLFTGPPADRRRFLDRLVLAVDASHGTR
VNALERALRSRNRVLEDGGDNRMLSAVEHELAELGVAVAAARLETVQRLAGEIAAHADETSPFPHADIALDGALERMLTD
APAVEVEDRYRALLQDNRPRDRAAGRTLEGPHLCDLVVAHGPKGLPAARCSTGEQKSLLIGLALSHARLVTAMQGLAPVV
LLDDVVAYLDAERRSGMFAALKALGAQVWMTGADPSAFAALDGAERFTVSPGAVNRSV

Sequences:

>Translated_378_residues
MTAPRARVRRLTLSRFRSYSAAQIEVADGPVVLTGPNGAGKTNILEAVSLLSPGRGLRRAALDAFAQAGGDGSWAVAAHV
EGALGPVDLGTGSDASRPDGSTRRCRIDRESVSSANAFLDHLKVVWLTPEMDGLFTGPPADRRRFLDRLVLAVDASHGTR
VNALERALRSRNRVLEDGGDNRMLSAVEHELAELGVAVAAARLETVQRLAGEIAAHADETSPFPHADIALDGALERMLTD
APAVEVEDRYRALLQDNRPRDRAAGRTLEGPHLCDLVVAHGPKGLPAARCSTGEQKSLLIGLALSHARLVTAMQGLAPVV
LLDDVVAYLDAERRSGMFAALKALGAQVWMTGADPSAFAALDGAERFTVSPGAVNRSV
>Mature_377_residues
TAPRARVRRLTLSRFRSYSAAQIEVADGPVVLTGPNGAGKTNILEAVSLLSPGRGLRRAALDAFAQAGGDGSWAVAAHVE
GALGPVDLGTGSDASRPDGSTRRCRIDRESVSSANAFLDHLKVVWLTPEMDGLFTGPPADRRRFLDRLVLAVDASHGTRV
NALERALRSRNRVLEDGGDNRMLSAVEHELAELGVAVAAARLETVQRLAGEIAAHADETSPFPHADIALDGALERMLTDA
PAVEVEDRYRALLQDNRPRDRAAGRTLEGPHLCDLVVAHGPKGLPAARCSTGEQKSLLIGLALSHARLVTAMQGLAPVVL
LDDVVAYLDAERRSGMFAALKALGAQVWMTGADPSAFAALDGAERFTVSPGAVNRSV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=378, Percent_Identity=28.3068783068783, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 39984; Mature: 39853

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRARVRRLTLSRFRSYSAAQIEVADGPVVLTGPNGAGKTNILEAVSLLSPGRGLRRA
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHH
ALDAFAQAGGDGSWAVAAHVEGALGPVDLGTGSDASRPDGSTRRCRIDRESVSSANAFLD
HHHHHHHCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHH
HLKVVWLTPEMDGLFTGPPADRRRFLDRLVLAVDASHGTRVNALERALRSRNRVLEDGGD
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCC
NRMLSAVEHELAELGVAVAAARLETVQRLAGEIAAHADETSPFPHADIALDGALERMLTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
APAVEVEDRYRALLQDNRPRDRAAGRTLEGPHLCDLVVAHGPKGLPAARCSTGEQKSLLI
CCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHEEE
GLALSHARLVTAMQGLAPVVLLDDVVAYLDAERRSGMFAALKALGAQVWMTGADPSAFAA
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHH
LDGAERFTVSPGAVNRSV
CCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TAPRARVRRLTLSRFRSYSAAQIEVADGPVVLTGPNGAGKTNILEAVSLLSPGRGLRRA
CCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHH
ALDAFAQAGGDGSWAVAAHVEGALGPVDLGTGSDASRPDGSTRRCRIDRESVSSANAFLD
HHHHHHHCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHH
HLKVVWLTPEMDGLFTGPPADRRRFLDRLVLAVDASHGTRVNALERALRSRNRVLEDGGD
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCC
NRMLSAVEHELAELGVAVAAARLETVQRLAGEIAAHADETSPFPHADIALDGALERMLTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
APAVEVEDRYRALLQDNRPRDRAAGRTLEGPHLCDLVVAHGPKGLPAARCSTGEQKSLLI
CCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCHHEEE
GLALSHARLVTAMQGLAPVVLLDDVVAYLDAERRSGMFAALKALGAQVWMTGADPSAFAA
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHH
LDGAERFTVSPGAVNRSV
CCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]