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The map label for this gene is braD [H]

Identifier: 158421821

GI number: 158421821

Start: 240486

End: 241436

Strand: Direct

Name: braD [H]

Synonym: AZC_0197

Alternate gene names: 158421821

Gene position: 240486-241436 (Clockwise)

Preceding gene: 158421818

Following gene: 158421822

Centisome position: 4.48

GC content: 67.09

Gene sequence:

>951_bases
ATGCCGCGCGCCGCATCGCCCAAGGGAGGGGCCATGGATGACATCGTCTTCCAGCAGATCGTCAACTGGATCACCCTTGG
CAGCATCTACGCCCTTATCGCGCTCGGCTTCAGCCTGCTGTTCGGCGTTCTGAACATCATCCATTTCTCCCACGGCGACG
TGTCGCTGCTGGCGCCCTTCCTCGCGCTCGCGGCGGTATCGGCCGTGGGATCGGCGCTGGGAGATGCCGCCTCCCCGGCC
GTCTTCCTGCTGGCGCTCGCCATTGCCATCGGCCTCACCGGCGTCATCGGCGTGCTGCTGGATATCGTCGTCATCCAGCG
CTTCCGGAAAGCGCCGGCGATGATGGCGCTGGTGGCGACCGTGGCGCTGGGCATCGTCCTGCGCGAGCTGATCCGTCACC
TGTTTCCCAATGGCAGCAACCCGCACGCCTTTCCGCGCCTCGTCTCGGGCGATATCACGCTCGGCCGGTTCAACGTGCCG
GTCTTCAGCCTGATGGTGGTCGCGGTGTCGGTCGCCTGCGTGTGCGCGCTGTTCCTGATGCTGAACGGCACGCGCCTCGG
TCTGCACATCAAGGCCGTCTCGCAGGACCGTGACGCCGCCCGGCTCATGGCCATCGATCCCGGCCGCATCTTCCGCGCCA
CCTTCTTCGTCGCGTCGGCCATCGGTGCCGTGGGCGGCCTGTTCTTCTCCAGCTATGCGGGCATGGCGCGCTTCGATTTC
AGCGTGGACGTGGGCCTCATCGGCTTCTCCGCCGCTGTGGTGGGCGGTCTCGGCTCCATGCCCGGCGCCATCATCGGCAG
CCTGCTGATCGCCGGGCTCGACACGCTGGTGCAGGCGGTGGTGCCGAACGGGGCGTCCTACCGCCTCGTCTTCGTCTTCC
TGCTTGTCATCGCCGTGCTGGTGTTCCGGCCGTCCGGCCTCATGGGCCGCACCCTCGTGGAGAAAGTGTGA

Upstream 100 bases:

>100_bases
TGCCCTAGCGTTGCTTTAGGGCAGATTTGATCCAGCGCATTGACGGGGACGCGCCTCGCACATCCCCCGCTCCTCGTGCT
TTTCCGGACAAGACGCAGGC

Downstream 100 bases:

>100_bases
TGACGGACAGCCTCGCCATGCGACGCTCCGCCTCCGCCCGCGTGGGCGACGCACTCACCGTCGTCGCGGTGGAACTGGTC
GGCGCGCTCTATCTGCGCCA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPRAASPKGGAMDDIVFQQIVNWITLGSIYALIALGFSLLFGVLNIIHFSHGDVSLLAPFLALAAVSAVGSALGDAASPA
VFLLALAIAIGLTGVIGVLLDIVVIQRFRKAPAMMALVATVALGIVLRELIRHLFPNGSNPHAFPRLVSGDITLGRFNVP
VFSLMVVAVSVACVCALFLMLNGTRLGLHIKAVSQDRDAARLMAIDPGRIFRATFFVASAIGAVGGLFFSSYAGMARFDF
SVDVGLIGFSAAVVGGLGSMPGAIIGSLLIAGLDTLVQAVVPNGASYRLVFVFLLVIAVLVFRPSGLMGRTLVEKV

Sequences:

>Translated_316_residues
MPRAASPKGGAMDDIVFQQIVNWITLGSIYALIALGFSLLFGVLNIIHFSHGDVSLLAPFLALAAVSAVGSALGDAASPA
VFLLALAIAIGLTGVIGVLLDIVVIQRFRKAPAMMALVATVALGIVLRELIRHLFPNGSNPHAFPRLVSGDITLGRFNVP
VFSLMVVAVSVACVCALFLMLNGTRLGLHIKAVSQDRDAARLMAIDPGRIFRATFFVASAIGAVGGLFFSSYAGMARFDF
SVDVGLIGFSAAVVGGLGSMPGAIIGSLLIAGLDTLVQAVVPNGASYRLVFVFLLVIAVLVFRPSGLMGRTLVEKV
>Mature_315_residues
PRAASPKGGAMDDIVFQQIVNWITLGSIYALIALGFSLLFGVLNIIHFSHGDVSLLAPFLALAAVSAVGSALGDAASPAV
FLLALAIAIGLTGVIGVLLDIVVIQRFRKAPAMMALVATVALGIVLRELIRHLFPNGSNPHAFPRLVSGDITLGRFNVPV
FSLMVVAVSVACVCALFLMLNGTRLGLHIKAVSQDRDAARLMAIDPGRIFRATFFVASAIGAVGGLFFSSYAGMARFDFS
VDVGLIGFSAAVVGGLGSMPGAIIGSLLIAGLDTLVQAVVPNGASYRLVFVFLLVIAVLVFRPSGLMGRTLVEKV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=307, Percent_Identity=30.9446254071661, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32861; Mature: 32730

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRAASPKGGAMDDIVFQQIVNWITLGSIYALIALGFSLLFGVLNIIHFSHGDVSLLAPF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LALAAVSAVGSALGDAASPAVFLLALAIAIGLTGVIGVLLDIVVIQRFRKAPAMMALVAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGIVLRELIRHLFPNGSNPHAFPRLVSGDITLGRFNVPVFSLMVVAVSVACVCALFLM
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
LNGTRLGLHIKAVSQDRDAARLMAIDPGRIFRATFFVASAIGAVGGLFFSSYAGMARFDF
HCCCEEEEEEEEECCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECC
SVDVGLIGFSAAVVGGLGSMPGAIIGSLLIAGLDTLVQAVVPNGASYRLVFVFLLVIAVL
EECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VFRPSGLMGRTLVEKV
HHCCCCCHHHHHHHCC
>Mature Secondary Structure 
PRAASPKGGAMDDIVFQQIVNWITLGSIYALIALGFSLLFGVLNIIHFSHGDVSLLAPF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LALAAVSAVGSALGDAASPAVFLLALAIAIGLTGVIGVLLDIVVIQRFRKAPAMMALVAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGIVLRELIRHLFPNGSNPHAFPRLVSGDITLGRFNVPVFSLMVVAVSVACVCALFLM
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
LNGTRLGLHIKAVSQDRDAARLMAIDPGRIFRATFFVASAIGAVGGLFFSSYAGMARFDF
HCCCEEEEEEEEECCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECC
SVDVGLIGFSAAVVGGLGSMPGAIIGSLLIAGLDTLVQAVVPNGASYRLVFVFLLVIAVL
EECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VFRPSGLMGRTLVEKV
HHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]