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The map label for this gene is nodW [H]

Identifier: 158421818

GI number: 158421818

Start: 236679

End: 237323

Strand: Direct

Name: nodW [H]

Synonym: AZC_0194

Alternate gene names: 158421818

Gene position: 236679-237323 (Clockwise)

Preceding gene: 158421817

Following gene: 158421821

Centisome position: 4.41

GC content: 62.48

Gene sequence:

>645_bases
GTGACCTCGTCAAACGATCCAAAGCCGGTCGTCTATATCGTTGACGACGATCACTCGGTACGAGCGGCGCTACAGGATCT
GTTCGCCTCGGTGACACTCGATGCCATGGCCTTTTCGTCGATCCGTGAATTCCTGGAGCACCCGCGCACGGCGGGTCCGG
CCTGTCTCGTGCTCGATGTCAGGATGCCCGCCCAGAGCGGGATGGATTTCTACCTGCAGATGGACAGCCTGGGCCTTCGC
ATGCCCGTCATCTTCATCACGGGCCATGGCGATATCGCGATGGGCGTGAGGGCCATCAAGGACGGCGCCGTCGACTTTCT
GACGAAGCCGTTCCGCGACCACGACCTGCTCGACGCCGTACACACCGCCATCGCGCGCGATCAGCAGAGGCTCCACCAGG
AGGAGATCCTCTCGGAGCTTCAGCGCCGATGGGAAAGCCTCAACACGGGCGAGCGCGATGTTCTCCGCCTGGTCGTCAGA
GGGCTCCTGAACAAGCAGATCGCGGCCGAACTTTCCGTCAAGGAGATCACGGTCAAGGTCCGGCGGGCCCGCGTGATGCA
GAAGATGCGGGCCGCGTCGTTTGCCGATCTCGTCCGCATGGCCGATCAACTTGAGCTCGGTGCTGAGACGCGTCGGAATT
CCTGA

Upstream 100 bases:

>100_bases
CGATCGTGGAGGCGAACGGCGGGCGCATCTGGATGCTTCCGGCCCCGCCAGAGGCGGGCGCAACGTTCGTCTTCAACCTT
CCGACAGCGGGTTCAGCGGC

Downstream 100 bases:

>100_bases
TCCGAGCCGAACAAGCCCCACCGCCGGGCGTTCGTCGCCCCTCCGTTCTGCTCACCGGCAACGCGAGGCAGCCGTCACGG
GGCGATCGCTCAGCGCATGC

Product: nodulation protein W, DNA-binding response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSSNDPKPVVYIVDDDHSVRAALQDLFASVTLDAMAFSSIREFLEHPRTAGPACLVLDVRMPAQSGMDFYLQMDSLGLR
MPVIFITGHGDIAMGVRAIKDGAVDFLTKPFRDHDLLDAVHTAIARDQQRLHQEEILSELQRRWESLNTGERDVLRLVVR
GLLNKQIAAELSVKEITVKVRRARVMQKMRAASFADLVRMADQLELGAETRRNS

Sequences:

>Translated_214_residues
MTSSNDPKPVVYIVDDDHSVRAALQDLFASVTLDAMAFSSIREFLEHPRTAGPACLVLDVRMPAQSGMDFYLQMDSLGLR
MPVIFITGHGDIAMGVRAIKDGAVDFLTKPFRDHDLLDAVHTAIARDQQRLHQEEILSELQRRWESLNTGERDVLRLVVR
GLLNKQIAAELSVKEITVKVRRARVMQKMRAASFADLVRMADQLELGAETRRNS
>Mature_213_residues
TSSNDPKPVVYIVDDDHSVRAALQDLFASVTLDAMAFSSIREFLEHPRTAGPACLVLDVRMPAQSGMDFYLQMDSLGLRM
PVIFITGHGDIAMGVRAIKDGAVDFLTKPFRDHDLLDAVHTAIARDQQRLHQEEILSELQRRWESLNTGERDVLRLVVRG
LLNKQIAAELSVKEITVKVRRARVMQKMRAASFADLVRMADQLELGAETRRNS

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=33.8842975206612, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790102, Length=191, Percent_Identity=30.3664921465969, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24055; Mature: 23924

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNDPKPVVYIVDDDHSVRAALQDLFASVTLDAMAFSSIREFLEHPRTAGPACLVLDV
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RMPAQSGMDFYLQMDSLGLRMPVIFITGHGDIAMGVRAIKDGAVDFLTKPFRDHDLLDAV
ECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HTAIARDQQRLHQEEILSELQRRWESLNTGERDVLRLVVRGLLNKQIAAELSVKEITVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRARVMQKMRAASFADLVRMADQLELGAETRRNS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
TSSNDPKPVVYIVDDDHSVRAALQDLFASVTLDAMAFSSIREFLEHPRTAGPACLVLDV
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RMPAQSGMDFYLQMDSLGLRMPVIFITGHGDIAMGVRAIKDGAVDFLTKPFRDHDLLDAV
ECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HTAIARDQQRLHQEEILSELQRRWESLNTGERDVLRLVVRGLLNKQIAAELSVKEITVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRARVMQKMRAASFADLVRMADQLELGAETRRNS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]