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The map label for this gene is braE [H]

Identifier: 158421822

GI number: 158421822

Start: 241433

End: 242611

Strand: Direct

Name: braE [H]

Synonym: AZC_0198

Alternate gene names: 158421822

Gene position: 241433-242611 (Clockwise)

Preceding gene: 158421821

Following gene: 158421823

Centisome position: 4.5

GC content: 66.07

Gene sequence:

>1179_bases
GTGATGACGGACAGCCTCGCCATGCGACGCTCCGCCTCCGCCCGCGTGGGCGACGCACTCACCGTCGTCGCGGTGGAACT
GGTCGGCGCGCTCTATCTGCGCCAGTTCCTGCTCACCGATGACTGGCGCCTCGTCATCGCCCTGCTGGCGGCCTTCGCGG
GCGCGCTCGTGCTGATGGAGCGGCAGCCGGCGCTGGAGGCGCGGATCGAAGGGGCGTTCGCGGCCCAGTCGCGCCTCGCC
ATGCTGCTCGGCATCGCCATCGTGCTAGCCTTCCCGTTCGCGCTGGAAGGCAACACCTATGCGCTGCATCTGTGCGTCAT
CTCCATGCTCTATGCCGTGCTGGCGCTCGCGCTCAATTTCCAGCTCGGCAGCGCCAATATCCCGAACTTCGCCACCGGCG
CGTCCTATGGCATCGGAGCCTATGCCTCGGCCCTGCTGGCGCTGAATTTCGGCGTCAGCTTCTGGCTGGCGCTGCCGGCC
GCCGCCATTGTAGCCACCGTGTTCGGCTTCCTGCTCGGCATCCCGTCCATGCGCACCCGCGACAGCTATCTGGCGCTGGT
GACCATCGCGTTCGGGGTCGTGGTCAACCAGCTTCTCAACAATCTGGATTTCACCGGCGGCCCGAACGGCCTCGTGGGCA
TTCCGGTGCCCGAGCTGTTCGGCCACTCCTTCGCCTCGCCGCTCGTGATCCTCGGCGCCGAGCTGCCGCCGCAGGCGAAT
TTCTACTACCTCTCCGCGCTGCTGGTCCTGGTCTCCATCCTGTTCGCCGAGCGGCTGCACGATTCCCGCGTAGGCCTTGC
CTGGAATGCGCTGCGGGCGGATGAGCTCGCCGCCCGCTGCCAGGGCATCAACGTCACCTGGTACAAGGTGCTGGCCTTCG
CGGTGGATGCCTTCCTCGCCGGCTTTGCAGGCACGATCTACGCCTTCTATGTGGGCTTCATCTCGCCGGACAACTTCACC
TTCCTCGTCTCGGTGACGATCATGACCATGGTGATCGCAGGCGGCATGGACAACATCCTCGGCGTGATCGTCGGCGCCTT
CCTGCTCACGCTGCTGCCGGAAAAGCTGCGTGCCTTCTCGGACTACCGCATCCTGTTCTTCGGCGTCACCGTCATCGCCT
TCCTCATGATCCGCCCCCAGGGGCTCTTTCCCCAGCGCCTGCGGCGCTACGGAGACTGA

Upstream 100 bases:

>100_bases
ACGGGGCGTCCTACCGCCTCGTCTTCGTCTTCCTGCTTGTCATCGCCGTGCTGGTGTTCCGGCCGTCCGGCCTCATGGGC
CGCACCCTCGTGGAGAAAGT

Downstream 100 bases:

>100_bases
CCGATGGGCTTCGACGCTTCTTCTCCCGTCGTTCTCGAGGGCGAGAACCTCACCATGCGCTTCGGCGGCATCGTCGCCAT
GGCGCAGGTGGAGTTCAGCC

Product: branched chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MMTDSLAMRRSASARVGDALTVVAVELVGALYLRQFLLTDDWRLVIALLAAFAGALVLMERQPALEARIEGAFAAQSRLA
MLLGIAIVLAFPFALEGNTYALHLCVISMLYAVLALALNFQLGSANIPNFATGASYGIGAYASALLALNFGVSFWLALPA
AAIVATVFGFLLGIPSMRTRDSYLALVTIAFGVVVNQLLNNLDFTGGPNGLVGIPVPELFGHSFASPLVILGAELPPQAN
FYYLSALLVLVSILFAERLHDSRVGLAWNALRADELAARCQGINVTWYKVLAFAVDAFLAGFAGTIYAFYVGFISPDNFT
FLVSVTIMTMVIAGGMDNILGVIVGAFLLTLLPEKLRAFSDYRILFFGVTVIAFLMIRPQGLFPQRLRRYGD

Sequences:

>Translated_392_residues
MMTDSLAMRRSASARVGDALTVVAVELVGALYLRQFLLTDDWRLVIALLAAFAGALVLMERQPALEARIEGAFAAQSRLA
MLLGIAIVLAFPFALEGNTYALHLCVISMLYAVLALALNFQLGSANIPNFATGASYGIGAYASALLALNFGVSFWLALPA
AAIVATVFGFLLGIPSMRTRDSYLALVTIAFGVVVNQLLNNLDFTGGPNGLVGIPVPELFGHSFASPLVILGAELPPQAN
FYYLSALLVLVSILFAERLHDSRVGLAWNALRADELAARCQGINVTWYKVLAFAVDAFLAGFAGTIYAFYVGFISPDNFT
FLVSVTIMTMVIAGGMDNILGVIVGAFLLTLLPEKLRAFSDYRILFFGVTVIAFLMIRPQGLFPQRLRRYGD
>Mature_392_residues
MMTDSLAMRRSASARVGDALTVVAVELVGALYLRQFLLTDDWRLVIALLAAFAGALVLMERQPALEARIEGAFAAQSRLA
MLLGIAIVLAFPFALEGNTYALHLCVISMLYAVLALALNFQLGSANIPNFATGASYGIGAYASALLALNFGVSFWLALPA
AAIVATVFGFLLGIPSMRTRDSYLALVTIAFGVVVNQLLNNLDFTGGPNGLVGIPVPELFGHSFASPLVILGAELPPQAN
FYYLSALLVLVSILFAERLHDSRVGLAWNALRADELAARCQGINVTWYKVLAFAVDAFLAGFAGTIYAFYVGFISPDNFT
FLVSVTIMTMVIAGGMDNILGVIVGAFLLTLLPEKLRAFSDYRILFFGVTVIAFLMIRPQGLFPQRLRRYGD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=38.1107491856677, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42171; Mature: 42171

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDSLAMRRSASARVGDALTVVAVELVGALYLRQFLLTDDWRLVIALLAAFAGALVLME
CCCCHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RQPALEARIEGAFAAQSRLAMLLGIAIVLAFPFALEGNTYALHLCVISMLYAVLALALNF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHC
QLGSANIPNFATGASYGIGAYASALLALNFGVSFWLALPAAAIVATVFGFLLGIPSMRTR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSYLALVTIAFGVVVNQLLNNLDFTGGPNGLVGIPVPELFGHSFASPLVILGAELPPQAN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHCCHHCCCCEEEECCCCCCCC
FYYLSALLVLVSILFAERLHDSRVGLAWNALRADELAARCQGINVTWYKVLAFAVDAFLA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GFAGTIYAFYVGFISPDNFTFLVSVTIMTMVIAGGMDNILGVIVGAFLLTLLPEKLRAFS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
DYRILFFGVTVIAFLMIRPQGLFPQRLRRYGD
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure
MMTDSLAMRRSASARVGDALTVVAVELVGALYLRQFLLTDDWRLVIALLAAFAGALVLME
CCCCHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RQPALEARIEGAFAAQSRLAMLLGIAIVLAFPFALEGNTYALHLCVISMLYAVLALALNF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHC
QLGSANIPNFATGASYGIGAYASALLALNFGVSFWLALPAAAIVATVFGFLLGIPSMRTR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSYLALVTIAFGVVVNQLLNNLDFTGGPNGLVGIPVPELFGHSFASPLVILGAELPPQAN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHCCHHCCCCEEEECCCCCCCC
FYYLSALLVLVSILFAERLHDSRVGLAWNALRADELAARCQGINVTWYKVLAFAVDAFLA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GFAGTIYAFYVGFISPDNFTFLVSVTIMTMVIAGGMDNILGVIVGAFLLTLLPEKLRAFS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
DYRILFFGVTVIAFLMIRPQGLFPQRLRRYGD
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]