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The map label for this gene is murD [H]

Identifier: 158421725

GI number: 158421725

Start: 118506

End: 120116

Strand: Reverse

Name: murD [H]

Synonym: AZC_0101

Alternate gene names: 158421725

Gene position: 120116-118506 (Counterclockwise)

Preceding gene: 158421728

Following gene: 158421723

Centisome position: 2.24

GC content: 70.08

Gene sequence:

>1611_bases
GTGTCGCGGCAAAGGGAAATGCCGCGATTCTGCTGTGCCTGCCAAATGCGGCGCCGCTCGGGTCGGCGGCTCCGTCATCC
TGCGAACTCGACGGCGGCGCGCAAGACGGTATTCTCCCGGCCGCTTCGCACGGTGGCCCGCGCCCCGCTGCACCACGACA
CTCACCGGCGCCGCATCCCTCGCGGGGCCGTCAGCTTTCGGATGTTCGGCGTCATGATTCCCGTCAGCACTTTCAAGGAC
CGCCGGGTGGCGGTGTTCGGCCTCGGCGGCTCGGGCCTTGCGACCGCGCAGGCGCTCCTTGCCGGTGGCGCGGAGGTGAT
CGGCTGGGATGACGGCGAGGCGGGCCGCAAGAAGGCAGAGGCCGCCGGCATCACGGTTTCGGATTTGCACGAGGCGGACT
GGTCGGGCTTTGCCGCTCTGGTGCTGGCGCCCGGCGTGCCGCTCACCCATCCGACCCCGCACTGGACGGTCGGGCTGGCG
CAGGCGGCCGGCGTCGAGATCATCGGCGACATCGAACTCTTCTGCCGCGAGCGCCGAGCACTGAAGCCCACCGCGCCTTT
TGCCGCCATCACCGGCACCAACGGCAAGTCCACCACCACCGCCCTGCTCTCGCACCTCATGAAGGTGGGCGGGCGTGATG
TGCAGATGGGCGGCAACATTGGCACCGCCATCCTCTCGCTGGAGCCGTTCAAGGCCGGGCGGGTGCATGTGGTCGAGTGC
TCCTCCTACCAGATCGACCTGGCCCCCGGCCTTGATCCCTCCGTCGGCATCCACCTCAACCTGACGCCCGACCATATCGA
CCGGCATGGCACCTTCGAGCATTACGGCGCGGTGAAGGAACGCCTCATCGCCGGCGTGCAGCCGGGCGGCACCGCCGTGG
TGGGCGTCGATGACGCGCCGTCCGCCGCCATCGCGGACCGGGCCGAGGCTGCCGGCATCCACGTCGTGCGCATCTCGGTG
AAGCAGCCGCTGGCGGACGGCATCTGGCTCGACGGCGAGACGCTGGTGGTGAGCGAGAAGGGTGCGGAGGTCTTCCGCCT
GCCGCTCACCGGCATCGGCTCGCTGCGCGGCGCGCACAATGCGCAGAACGCCGCCGCCGCCATGGCCGCCGCGCGGGGCC
TCGGCCTTGCCTATGAGGTAATCGCGGTAGGGATGCAGCGCTTCCCCGGCCTCGCCCATCGCATGGAGCAGGTGGGCACG
CGGGGCAAGGTGCTGTTCGTCAACGACAGCAAGGCCACCAATGCGGATGCGGCCGAGCGGGCGCTGGCCAGCTTCTCCGA
CGTCTTCTGGATCGCAGGCGGCAAGCCCAAGGCGGGCGGCATCACGCCGCTGAAGCCCTTCTTCCCGCGCGTGCGCAAGG
CCTATCTCATCGGCGAGGCGGCGGCGGACTTTGCCGCCACCCTGGGCGATGTGCCCCATGAGGTGGTGGGCACACTGGAC
AGGGCCGTCGCTGCTGCCGCGCGGGATGCGGCGGCCTCAAGCCTCGACCATCCCGTGGTGCTGCTCTCGCCCGCCTGCGC
CAGCTTCGACCAGTATCCGAACTTCGAGGTGCGCGGCGATGCCTTCCGCCATCTGGTGGAGGAACTGCCGCAGGAGACCG
CTCCGGCCTGA

Upstream 100 bases:

>100_bases
ACCGCCGCCACCACCTGCGCGGGCTTTGCGGCGATCTCGCCGGCGATGATGCGGGCGATGTCGGCAGAGGGTGCGGCCAT
GAAGAAGTCCTTGAGCTTAT

Downstream 100 bases:

>100_bases
TCCCGAGGGCGGGCGGAGCGGCCTTCACCAGATCCGGCCCCGTGCTAGCGCGGGGTGATCTTCTGGTAGGTGTTCTTCAG
GTCGTCCTGCAGCACCGTCG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSRQREMPRFCCACQMRRRSGRRLRHPANSTAARKTVFSRPLRTVARAPLHHDTHRRRIPRGAVSFRMFGVMIPVSTFKD
RRVAVFGLGGSGLATAQALLAGGAEVIGWDDGEAGRKKAEAAGITVSDLHEADWSGFAALVLAPGVPLTHPTPHWTVGLA
QAAGVEIIGDIELFCRERRALKPTAPFAAITGTNGKSTTTALLSHLMKVGGRDVQMGGNIGTAILSLEPFKAGRVHVVEC
SSYQIDLAPGLDPSVGIHLNLTPDHIDRHGTFEHYGAVKERLIAGVQPGGTAVVGVDDAPSAAIADRAEAAGIHVVRISV
KQPLADGIWLDGETLVVSEKGAEVFRLPLTGIGSLRGAHNAQNAAAAMAAARGLGLAYEVIAVGMQRFPGLAHRMEQVGT
RGKVLFVNDSKATNADAAERALASFSDVFWIAGGKPKAGGITPLKPFFPRVRKAYLIGEAAADFAATLGDVPHEVVGTLD
RAVAAAARDAAASSLDHPVVLLSPACASFDQYPNFEVRGDAFRHLVEELPQETAPA

Sequences:

>Translated_536_residues
MSRQREMPRFCCACQMRRRSGRRLRHPANSTAARKTVFSRPLRTVARAPLHHDTHRRRIPRGAVSFRMFGVMIPVSTFKD
RRVAVFGLGGSGLATAQALLAGGAEVIGWDDGEAGRKKAEAAGITVSDLHEADWSGFAALVLAPGVPLTHPTPHWTVGLA
QAAGVEIIGDIELFCRERRALKPTAPFAAITGTNGKSTTTALLSHLMKVGGRDVQMGGNIGTAILSLEPFKAGRVHVVEC
SSYQIDLAPGLDPSVGIHLNLTPDHIDRHGTFEHYGAVKERLIAGVQPGGTAVVGVDDAPSAAIADRAEAAGIHVVRISV
KQPLADGIWLDGETLVVSEKGAEVFRLPLTGIGSLRGAHNAQNAAAAMAAARGLGLAYEVIAVGMQRFPGLAHRMEQVGT
RGKVLFVNDSKATNADAAERALASFSDVFWIAGGKPKAGGITPLKPFFPRVRKAYLIGEAAADFAATLGDVPHEVVGTLD
RAVAAAARDAAASSLDHPVVLLSPACASFDQYPNFEVRGDAFRHLVEELPQETAPA
>Mature_535_residues
SRQREMPRFCCACQMRRRSGRRLRHPANSTAARKTVFSRPLRTVARAPLHHDTHRRRIPRGAVSFRMFGVMIPVSTFKDR
RVAVFGLGGSGLATAQALLAGGAEVIGWDDGEAGRKKAEAAGITVSDLHEADWSGFAALVLAPGVPLTHPTPHWTVGLAQ
AAGVEIIGDIELFCRERRALKPTAPFAAITGTNGKSTTTALLSHLMKVGGRDVQMGGNIGTAILSLEPFKAGRVHVVECS
SYQIDLAPGLDPSVGIHLNLTPDHIDRHGTFEHYGAVKERLIAGVQPGGTAVVGVDDAPSAAIADRAEAAGIHVVRISVK
QPLADGIWLDGETLVVSEKGAEVFRLPLTGIGSLRGAHNAQNAAAAMAAARGLGLAYEVIAVGMQRFPGLAHRMEQVGTR
GKVLFVNDSKATNADAAERALASFSDVFWIAGGKPKAGGITPLKPFFPRVRKAYLIGEAAADFAATLGDVPHEVVGTLDR
AVAAAARDAAASSLDHPVVLLSPACASFDQYPNFEVRGDAFRHLVEELPQETAPA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=468, Percent_Identity=33.974358974359, Blast_Score=172, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013116
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF07991 IlvN; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 56648; Mature: 56517

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQREMPRFCCACQMRRRSGRRLRHPANSTAARKTVFSRPLRTVARAPLHHDTHRRRIP
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
RGAVSFRMFGVMIPVSTFKDRRVAVFGLGGSGLATAQALLAGGAEVIGWDDGEAGRKKAE
CCHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHH
AAGITVSDLHEADWSGFAALVLAPGVPLTHPTPHWTVGLAQAAGVEIIGDIELFCRERRA
HCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCEEEEECHHHHHHHHHC
LKPTAPFAAITGTNGKSTTTALLSHLMKVGGRDVQMGGNIGTAILSLEPFKAGRVHVVEC
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCEEEEEE
SSYQIDLAPGLDPSVGIHLNLTPDHIDRHGTFEHYGAVKERLIAGVQPGGTAVVGVDDAP
CCEEEEECCCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
SAAIADRAEAAGIHVVRISVKQPLADGIWLDGETLVVSEKGAEVFRLPLTGIGSLRGAHN
CHHHHHHHHHCCEEEEEEEECCCCCCCEEECCCEEEEECCCCEEEEECCCCCCCCCCCCC
AQNAAAAMAAARGLGLAYEVIAVGMQRFPGLAHRMEQVGTRGKVLFVNDSKATNADAAER
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
ALASFSDVFWIAGGKPKAGGITPLKPFFPRVRKAYLIGEAAADFAATLGDVPHEVVGTLD
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RAVAAAARDAAASSLDHPVVLLSPACASFDQYPNFEVRGDAFRHLVEELPQETAPA
HHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRQREMPRFCCACQMRRRSGRRLRHPANSTAARKTVFSRPLRTVARAPLHHDTHRRRIP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
RGAVSFRMFGVMIPVSTFKDRRVAVFGLGGSGLATAQALLAGGAEVIGWDDGEAGRKKAE
CCHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHH
AAGITVSDLHEADWSGFAALVLAPGVPLTHPTPHWTVGLAQAAGVEIIGDIELFCRERRA
HCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCEEEEECHHHHHHHHHC
LKPTAPFAAITGTNGKSTTTALLSHLMKVGGRDVQMGGNIGTAILSLEPFKAGRVHVVEC
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCEEEEEE
SSYQIDLAPGLDPSVGIHLNLTPDHIDRHGTFEHYGAVKERLIAGVQPGGTAVVGVDDAP
CCEEEEECCCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
SAAIADRAEAAGIHVVRISVKQPLADGIWLDGETLVVSEKGAEVFRLPLTGIGSLRGAHN
CHHHHHHHHHCCEEEEEEEECCCCCCCEEECCCEEEEECCCCEEEEECCCCCCCCCCCCC
AQNAAAAMAAARGLGLAYEVIAVGMQRFPGLAHRMEQVGTRGKVLFVNDSKATNADAAER
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
ALASFSDVFWIAGGKPKAGGITPLKPFFPRVRKAYLIGEAAADFAATLGDVPHEVVGTLD
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RAVAAAARDAAASSLDHPVVLLSPACASFDQYPNFEVRGDAFRHLVEELPQETAPA
HHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]