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The map label for this gene is ksgA

Identifier: 15836590

GI number: 15836590

Start: 1211182

End: 1212015

Strand: Direct

Name: ksgA

Synonym: CPj1059

Alternate gene names: 15836590

Gene position: 1211182-1212015 (Clockwise)

Preceding gene: 15836589

Following gene: 15836594

Centisome position: 98.75

GC content: 41.73

Gene sequence:

>834_bases
GTGACAAGAAGTTCTCCTGCACAACTCTCCCGGTTTCTTTCTGAAATTCAAAATAAGCCGAAGAAAAGCCTCTCTCAAAA
TTTCTTAGTGGATCAGAATATTGTCAAAAAAATTGTGGCAACCTCTGAGGTGATTCCTCAAGATTGGGTGTTGGAGATCG
GCCCAGGATTTGGAGCCCTTACAGAAGAACTCATAGCTGCAGGAGCTCAGGTCATTGCTATTGAAAAAGACCCGATGTTT
GCGCCATCTTTAGAAGAGCTCCCTATCCGCTTAGAAATTATCGATGCCTGTAAATACCCGTTAGATCAGCTTCAGGAATA
TAAGACTTTGGGAAAAGGTCGAGTAGTAGCAAATCTTCCCTACCATATTACCACTCCATTGCTTACCAAGTTGTTTCTGG
AAGCCCCAGATTTCTGGAAAACAGTGACGGTTATGGTCCAAGATGAAGTGGCCCGGCGTATTGTAGCCCAGCCAGGGGGT
AGGGATTATGGGTCTCTAACGATCTTTTTGCAGTTTTTTGCTGATATACACTATGCTTTTAAAGTTTCTGCTTCATGCTT
CTATCCAAAACCGCAAGTGCAATCTGCAGTCATTCATATGAAAGTCAAAGAAACACTTCCTCTATCAGACGAGGAGATTC
CTGTATTTTTTACTTTAACAAGAACTGCATTTCAACAGCGTCGAAAAGTACTCGCTAATACTCTAAAAGGCCTCTATCCT
AAAGAGCAAGTGGAACAAGCTTTGAAGGAATTAGGATTACTTCTAAATGTGCGTCCTGAAGTATTGAGTCTTAATGATTA
TCTTGCTCTCTTCCATAAGATGCAAGCGGGTTAA

Upstream 100 bases:

>100_bases
AAGACAGATTACGCAAGCGCTACGGCTATGATGCTTCTATGATTGCGAAACTTCTTTCTGAAGAAGCTCCACCTCTATTT
TCCTTATTATAGGGGATTGA

Downstream 100 bases:

>100_bases
AGGTACTTTCCCTTCTAAGGAAATTACAATCTCTAGGGATAATTAAACTCTGACGTCTCCAACAGTCTGCTTTTTTGATC
TGAAGTTGAAATGAGTGAGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTRSSPAQLSRFLSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGALTEELIAAGAQVIAIEKDPMF
APSLEELPIRLEIIDACKYPLDQLQEYKTLGKGRVVANLPYHITTPLLTKLFLEAPDFWKTVTVMVQDEVARRIVAQPGG
RDYGSLTIFLQFFADIHYAFKVSASCFYPKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLKGLYP
KEQVEQALKELGLLLNVRPEVLSLNDYLALFHKMQAG

Sequences:

>Translated_277_residues
MTRSSPAQLSRFLSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGALTEELIAAGAQVIAIEKDPMF
APSLEELPIRLEIIDACKYPLDQLQEYKTLGKGRVVANLPYHITTPLLTKLFLEAPDFWKTVTVMVQDEVARRIVAQPGG
RDYGSLTIFLQFFADIHYAFKVSASCFYPKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLKGLYP
KEQVEQALKELGLLLNVRPEVLSLNDYLALFHKMQAG
>Mature_276_residues
TRSSPAQLSRFLSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGALTEELIAAGAQVIAIEKDPMFA
PSLEELPIRLEIIDACKYPLDQLQEYKTLGKGRVVANLPYHITTPLLTKLFLEAPDFWKTVTVMVQDEVARRIVAQPGGR
DYGSLTIFLQFFADIHYAFKVSASCFYPKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLKGLYPK
EQVEQALKELGLLLNVRPEVLSLNDYLALFHKMQAG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=28.673835125448, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7657198, Length=257, Percent_Identity=29.9610894941634, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=34.4961240310078, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=30.1694915254237, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=221, Percent_Identity=32.1266968325792, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=235, Percent_Identity=29.3617021276596, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358017, Length=266, Percent_Identity=30.0751879699248, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357273, Length=259, Percent_Identity=29.3436293436293, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CHLPN (Q9Z6K0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   H72001
- PIR:   H86622
- RefSeq:   NP_225253.1
- RefSeq:   NP_301114.1
- RefSeq:   NP_445330.1
- RefSeq:   NP_877372.1
- ProteinModelPortal:   Q9Z6K0
- SMR:   Q9Z6K0
- GeneID:   1467780
- GeneID:   895577
- GeneID:   919826
- GeneID:   963117
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0791
- KEGG:   cpn:CPn1059
- KEGG:   cpt:CpB1101
- TIGR:   CP_0791
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q9Z6K0
- ProtClustDB:   PRK00274
- BioCyc:   CPNE115711:CP_0791-MONOMER
- BioCyc:   CPNE115713:CPN1059-MONOMER
- BioCyc:   CPNE138677:CPJ1059-MONOMER
- BioCyc:   CPNE182082:CPB1101-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31218; Mature: 31087

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 95-95 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSSPAQLSRFLSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGAL
CCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHH
TEELIAAGAQVIAIEKDPMFAPSLEELPIRLEIIDACKYPLDQLQEYKTLGKGRVVANLP
HHHHHHCCCEEEEEECCCCCCCCHHHCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
YHITTPLLTKLFLEAPDFWKTVTVMVQDEVARRIVAQPGGRDYGSLTIFLQFFADIHYAF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KVSASCFYPKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLKGLYP
EECCEECCCCCHHHHHHHEEEHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
KEQVEQALKELGLLLNVRPEVLSLNDYLALFHKMQAG
HHHHHHHHHHHCHHEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRSSPAQLSRFLSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGAL
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHH
TEELIAAGAQVIAIEKDPMFAPSLEELPIRLEIIDACKYPLDQLQEYKTLGKGRVVANLP
HHHHHHCCCEEEEEECCCCCCCCHHHCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
YHITTPLLTKLFLEAPDFWKTVTVMVQDEVARRIVAQPGGRDYGSLTIFLQFFADIHYAF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KVSASCFYPKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLKGLYP
EECCEECCCCCHHHHHHHEEEHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
KEQVEQALKELGLLLNVRPEVLSLNDYLALFHKMQAG
HHHHHHHHHHHCHHEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362