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The map label for this gene is tpiA

Identifier: 15836594

GI number: 15836594

Start: 1216285

End: 1217049

Strand: Direct

Name: tpiA

Synonym: CPj1063

Alternate gene names: 15836594

Gene position: 1216285-1217049 (Clockwise)

Preceding gene: 15836590

Following gene: 15836595

Centisome position: 99.16

GC content: 43.14

Gene sequence:

>765_bases
ATGACAAGGCAGAGTTATGTTTTGGGCAATTGGAAAATGCACAAAACAATCCAAGAAGCTAAAGAGTATGTTCAAACATT
AGCTTCTTTACTACAAGGAGAACCTCTTTCCTGCACTATAGGCATAGCTTCTCCATTTACCTCTTTGAGAGCGATTCATG
AGATGATAAACACTACGGGAGCTTTTCTCTGGTTGGGAGCACAAAATGTCCATCCCGAGCTTTCGGGTGCTTTTACTGGA
GAAATTTCCTTACCTATGCTTAAGGAGGTAGGAGTGGAATTTGTTTTAGTAGGTCACTCCGAGCGTCGTCATATTTTTGG
AGAGAGTGATGCCCTTATTGCTTCAAAGGTAAAGTCTGTAGCTCAGGCGGGACTCGTGCCTGTTCTTTGTGTTGGAGAGA
GCTTAGAAGTTCGTGAAGAGGGAAAGGCGCATCAGGTAATCAAAAAACAGTTGCTTTTGGGATTGGAACAGATGGATAAT
GGTTCCGAATTTTTGATCGCCTATGAACCAGTATGGGCTATCGGCACAGGGAAGGTGGCAGAAGCTTCGGATGTGCAAGA
TATTCATATGTTTTGTCGTGAGGTAGTGGCAGAGAGGTTCTCAGAAGCTACAGCTGAAGAGATTTCGATTTTGTACGGAG
GATCTGTGAAGGTCGATAATGCTCAGCGATTTGGGCAATGTAGCGACGTCGATGGTCTTTTAGTTGGCGGAGCTTCTTTA
GAAGGGCAAAGTTTTTTTGAAGTCGCTAAAAATTTTAATGTATAA

Upstream 100 bases:

>100_bases
ACTTTAGAGTAAGTGATTTTCTGATAGAAAACTATTAAAAAATCATTGATTCTGTCGGGAAAGTATGCGGATAAAATTCA
GAGAGAATAAGGAGAGGAAG

Downstream 100 bases:

>100_bases
TTTGTGAGAGTTATGAGATTTTTTTGTCTATTTTTTCTTGGGTTCCTAGGATCTTTTCATTGTGTTGCTGAAGACAAGGG
CGTGGATTTATTTGGAGTCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTRQSYVLGNWKMHKTIQEAKEYVQTLASLLQGEPLSCTIGIASPFTSLRAIHEMINTTGAFLWLGAQNVHPELSGAFTG
EISLPMLKEVGVEFVLVGHSERRHIFGESDALIASKVKSVAQAGLVPVLCVGESLEVREEGKAHQVIKKQLLLGLEQMDN
GSEFLIAYEPVWAIGTGKVAEASDVQDIHMFCREVVAERFSEATAEEISILYGGSVKVDNAQRFGQCSDVDGLLVGGASL
EGQSFFEVAKNFNV

Sequences:

>Translated_254_residues
MTRQSYVLGNWKMHKTIQEAKEYVQTLASLLQGEPLSCTIGIASPFTSLRAIHEMINTTGAFLWLGAQNVHPELSGAFTG
EISLPMLKEVGVEFVLVGHSERRHIFGESDALIASKVKSVAQAGLVPVLCVGESLEVREEGKAHQVIKKQLLLGLEQMDN
GSEFLIAYEPVWAIGTGKVAEASDVQDIHMFCREVVAERFSEATAEEISILYGGSVKVDNAQRFGQCSDVDGLLVGGASL
EGQSFFEVAKNFNV
>Mature_253_residues
TRQSYVLGNWKMHKTIQEAKEYVQTLASLLQGEPLSCTIGIASPFTSLRAIHEMINTTGAFLWLGAQNVHPELSGAFTGE
ISLPMLKEVGVEFVLVGHSERRHIFGESDALIASKVKSVAQAGLVPVLCVGESLEVREEGKAHQVIKKQLLLGLEQMDNG
SEFLIAYEPVWAIGTGKVAEASDVQDIHMFCREVVAERFSEATAEEISILYGGSVKVDNAQRFGQCSDVDGLLVGGASLE
GQSFFEVAKNFNV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=240, Percent_Identity=39.1666666666667, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI226529917, Length=240, Percent_Identity=39.1666666666667, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=41.4342629482072, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=41.9087136929461, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=255, Percent_Identity=40.7843137254902, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572004, Length=243, Percent_Identity=41.1522633744856, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28572008, Length=243, Percent_Identity=41.1522633744856, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI28572006, Length=243, Percent_Identity=41.1522633744856, Blast_Score=184, Evalue=5e-47,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27601; Mature: 27470

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQSYVLGNWKMHKTIQEAKEYVQTLASLLQGEPLSCTIGIASPFTSLRAIHEMINTTG
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC
AFLWLGAQNVHPELSGAFTGEISLPMLKEVGVEFVLVGHSERRHIFGESDALIASKVKSV
EEEEECCCCCCCCCCCCEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AQAGLVPVLCVGESLEVREEGKAHQVIKKQLLLGLEQMDNGSEFLIAYEPVWAIGTGKVA
HHHCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCC
EASDVQDIHMFCREVVAERFSEATAEEISILYGGSVKVDNAQRFGQCSDVDGLLVGGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEECCHHHCCCCCCCCCEEEECCCC
EGQSFFEVAKNFNV
CCHHHHHHHHCCCC
>Mature Secondary Structure 
TRQSYVLGNWKMHKTIQEAKEYVQTLASLLQGEPLSCTIGIASPFTSLRAIHEMINTTG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC
AFLWLGAQNVHPELSGAFTGEISLPMLKEVGVEFVLVGHSERRHIFGESDALIASKVKSV
EEEEECCCCCCCCCCCCEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AQAGLVPVLCVGESLEVREEGKAHQVIKKQLLLGLEQMDNGSEFLIAYEPVWAIGTGKVA
HHHCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCC
EASDVQDIHMFCREVVAERFSEATAEEISILYGGSVKVDNAQRFGQCSDVDGLLVGGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEECCHHHCCCCCCCCCEEEECCCC
EGQSFFEVAKNFNV
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]